Quinones in chlorosomes of green sulfur bacteria and their role in the redox-dependent fluorescence studied in chlorosome-like bacteriochlorophyll c aggregates

Isolation and Characterization of Homodimeric Type-I Reaction Center Complex from Candidatus Chloracidobacterium thermophilum, an Aerobic Chlorophototroph

“…5A, black line) was typical of BChl a; this pigment had the same elution time as authentic BChl a P derived from C. tepidum (29). Thus, the BChl a in Ca.…”

Characterization of a Plant-like Protochlorophyllide a Divinyl Reductase in Green Sulfur Bacteria

“…For the HPLC and HPLC-MS analyses, cells from 1 ml of a dense culture were pelleted by centrifugation, and the pigments were extracted by sonication in acetone: methanol (7:2, v/v). The pigment extract was subjected to reverse-phase HPLC as described (20) to resolve the different BChl c species as well as BChl a and Chl a. ChemStation Rev. A.10.03 software by Agilent Technologies was used to analyze the chromatograms and the absorption spectra from the different samples and the eluants.…”

“…The pigments were extracted again with acetone: methanol (7:2, v/v) immediately before injection in the HPLC. The method used to separate these compounds was a modified version of the method described (20). Solvent A (62.5% water, 21% methanol, 16.5% acetonitrile, 10 mM ammonium acetate) was used as the stationary phase and solvent B (50% methanol, 20% acetonitrile, 30% ethyl acetate) was used as the mobile phase using the following gradient.…”

Menaquinone as Well as Ubiquinone as a Crucial Component in the Escherichia coli Respiratory Chain

“…MK occurs in chlorosomes of green photosynthetic bacteria (Frigaard et al, 1997;Frigaard et al, 1998;Kim et al, 2007). A small amount of MK-7 with a large amount of chlorobiumquinone is found in chlorosomes of the green sulfur bacterium Chlorobium tepidum (Frigaard et al, 1997).…”