Quinone-Based Polymers for Label-Free and Reagentless Electrochemical Immunosensors: Application to Proteins, Antibodies and Pesticides Detection

Piro, Benoı̂t; Reisberg, S.; Anquetin, Guillaume; Duc, Huynh Thien; Pham, Minh

doi:10.3390/bios3010058

Cited by 27 publications

(11 citation statements)

References 66 publications

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“…For example, ferrocene and ferrocenemethanol have also been used as redox probes for pathogen detection. Ruthenium(III)/ruthenium (II) (Schrattenecker et al 2019) and immobilized quinone pairs (Piro et al 2013) are also potentially useful alternatives. Biosensors that use impedance-based methods and whose impedance response can be modeled using equivalent circuit models can be used to calculate the capacitance of the electric double layer.…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

619

358

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A B S T R A C TRecent advances in electrochemical biosensors for pathogen detection are reviewed. Electrochemical biosensors for pathogen detection are broadly reviewed in terms of transduction elements, biorecognition elements, electrochemical techniques, and biosensor performance. Transduction elements are discussed in terms of electrode material and form factor. Biorecognition elements for pathogen detection, including antibodies, aptamers, and imprinted polymers, are discussed in terms of availability, production, and immobilization approach. Emerging areas of electrochemical biosensor design are reviewed, including electrode modification and transducer integration. Measurement formats for pathogen detection are classified in terms of sample preparation and secondary binding steps. Applications of electrochemical biosensors for the detection of pathogens in food and water safety, medical diagnostics, environmental monitoring, and bio-threat applications are highlighted. Future directions and challenges of electrochemical biosensors for pathogen detection are discussed, including wearable and conformal biosensors, detection of plant pathogens, multiplexed detection, reusable biosensors for process monitoring applications, and low-cost, disposable biosensors.

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Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

619

358

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“…Overall, our results show that the sequential use of PpAzoR and CotA results in the conversion of all azo dyes into structurally different building blocks, aromatic amines, naphthoquinones, phenoxazinones, and phenazines. These are biological active motifs of antibiotics ( McDonald et al, 1999 ), antitumor agents ( Corona et al, 2009 ; Vairavelu et al, 2014 ), and pesticides ( Piro et al, 2013 ). They are also useful precursors for the manufacturing of pharmaceuticals ( Cholo et al, 2012 ), fine chemicals ( Forte et al, 2010 ; Morel and Christie, 2011 ), and biosensors ( Piro et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

Wasteful Azo Dyes as a Source of Biologically Active Building Blocks

Fernándes

Pinto

Bonardo

et al. 2021

Front. Bioeng. Biotechnol.

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In this work, an environment-friendly enzymatic strategy was developed for the valorisation of dye-containing wastewaters. We set up biocatalytic processes for the conversion of azo dyes representative of the main classes used in the textile industry into valuable aromatic compounds: aromatic amines, phenoxazinones, phenazines, and naphthoquinones. First, purified preparations of PpAzoR azoreductase efficiently reduced mordant, acid, reactive, and direct azo dyes into aromatic amines, and CotA-laccase oxidised these compounds into phenazines, phenoxazinones, and naphthoquinones. Second, whole cells containing the overproduced enzymes were utilised in the two-step enzymatic conversion of the model mordant black 9 dye into sodium 2-amino-3-oxo-3H-phenoxazine-8-sulphonate, allowing to overcome the drawbacks associated with the use of expensive purified enzymes, co-factors, or exquisite reaction conditions. Third, cells immobilised in sodium alginate allowed recycling the biocatalysts and achieving very good to excellent final phenoxazine product yields (up to 80%) in water and with less impurities in the final reaction mixtures. Finally, one-pot systems using recycled immobilised cells co-producing both enzymes resulted in the highest phenoxazinone yields (90%) through the sequential use of static and stirring conditions, controlling the oxygenation of reaction mixtures and the successive activity of azoreductase (anaerobic) and laccase (aerobic).

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“…Electrochemical (EC) immunosensors have been used for detecting antibody against virus, 47,48 cancer biomarkers, 49,50 pesticides, 51 microorganisms, 52 and protein biomarkers. 53 The sensing principle of a typical electrochemical immunosensor is shown in Fig.…”

Section: Electrical Biomarker Detection Methodsmentioning

confidence: 99%

Lab-on-a-chip electrical multiplexing techniques for cellular and molecular biomarker detection

Liu

Zhe

2018

Biomicrofluidics

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Signal multiplexing is vital to develop lab-on-a-chip devices that can detect and quantify multiple cellular and molecular biomarkers with high throughput, short analysis time, and low cost. Electrical detection of biomarkers has been widely used in lab-on-a-chip devices because it requires less external equipment and simple signal processing and provides higher scalability. Various electrical multiplexing for lab-on-a-chip devices have been developed for comprehensive, high throughput, and rapid analysis of biomarkers. In this paper, we first briefly introduce the widely used electrochemical and electrical impedance sensing methods. Next, we focus on reviewing various electrical multiplexing techniques that had achieved certain successes on rapid cellular and molecular biomarker detection, including direct methods (spatial and time multiplexing), and emerging technologies (frequency, codes, particle-based multiplexing). Lastly, the future opportunities and challenges on electrical multiplexing techniques are also discussed.

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Quinone-Based Polymers for Label-Free and Reagentless Electrochemical Immunosensors: Application to Proteins, Antibodies and Pesticides Detection

Cited by 27 publications

References 66 publications

Electrochemical biosensors for pathogen detection

Electrochemical biosensors for pathogen detection

Wasteful Azo Dyes as a Source of Biologically Active Building Blocks

Lab-on-a-chip electrical multiplexing techniques for cellular and molecular biomarker detection

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