Quinolone-induced differential modification of IL-1α and IL-1β production by LPS-stimulated human monocytes

Bailly, Sabine; Mahé, Yann F.; Ferruà, Bernard; Fay, Michèle; Tursz, Thomas; Wakasugi, Hiro; Gougerot‐Pocidalo, Marie‐Anne

doi:10.1016/0008-8749(90)90025-m

Cited by 42 publications

(35 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the group of patients with noninfectious shock, a reduced production of IL-1a was also noticed, whereas this was not the case for IL-1 f, IL-6, and TNFa. This observation confirms the fact that the regulation of IL-1 a and IL-l 3 productions are under distinct pathways (65). This downregulation of cytokine production was especially significant for patients with gram-negative infections.…”

Section: Discussionsupporting

confidence: 84%

Dysregulation of in vitro cytokine production by monocytes during sepsis.

Muñoz¹,

Carlet²,

Fitting³

et al. 1991

J. Clin. Invest.

689

396

View full text Add to dashboard Cite

The production by monocytes of interleukin-la (IL-la), interleukin-1lj# ), , and tumor necrosis factor alpha (TNFa) in intensive care unit (ICU) patients with sepsis syndrome (n = 23) or noninfectious shock (n = 6) is reported. Plasma cytokines, cell-associated cytokines within freshly isolated monocytes and LPS-induced in vitro cytokine production were assessed at admission and at regular intervals during ICU stay. TNFa and IL-6 were the most frequently detected circulating cytokines. Despite the fact that IL-la is the main cytokine found within monocytes upon in vitro activation of cells from healthy individuals, it was very rarely detected within freshly isolated monocytes from septic patients, and levels of cell-associated IL-1,@ were lower than those of TNFa. Cell-associated IL-1,6 and TNFa were not correlated with corresponding levels in plasma. Upon LPS stimulation, we observed a profound decrease of in vitro IL-la production by monocytes in all patients, and of IL-1if, IL-6, and TNFa in septic patients. This reduced LPS-induced production of cytokines was most pronounced in patients with gram-negative infections. Finally, monocytes from survival patients, but not from nonsurvival ones recovered their capacity to produce normal amounts of cytokines upon LPS stimulation. In conclusion, our data indicate an in vivo activation of circulating monocytes during sepsis as well as in noninfectious shock and suggest that complex regulatory mechanisms can downregulate the production of cytokines by monocytes during severe infections.

show abstract

Section: Discussionsupporting

confidence: 84%

Dysregulation of in vitro cytokine production by monocytes during sepsis.

Muñoz¹,

Carlet²,

Fitting³

et al. 1991

J. Clin. Invest.

689

396

View full text Add to dashboard Cite

show abstract

“…This finding was unexpected based on previous evidence that both IL-6 and TNF-a are typically regulated by the same transcription factor, NF-kB. 35 While not tested here, AngII has been shown to promote prostaglandin production, in particular prostaglandin E2 (PGE2), which in turn has been shown to inhibit the production of TNF-a while enhancing IL-6 production, thus supporting our findings 36,37 The pro-inflammatory nature of IL-6 is supported by evidence that the expression of IL-1, a well-known pro-inflammatory cytokine, was also upregulated by AngII exposure. 38 In summary, we provide evidence that there is variable expression of AT 1 R in subsets of purified monocytes, with greater expression seen in CD14 + /CD16 + monocytes.…”

Section: Mediasupporting

confidence: 79%

Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II

Gelinas

Falkenham

Oxner

et al. 2011

J Renin Angiotensin Aldosterone Syst

View full text Add to dashboard Cite

Hypothesis: Monocytes produce pro-inflammatory cytokines in response to Angiotensin II (AngII). Introduction: AngII has been suggested by many to be pro-inflammatory and likely to contribute to the migration of leukocytes in patients with cardiovascular conditions. Materials and methods: Monocytes were purified from peripheral blood mononuclear cells (PBMCs) by negative selection using antibodies conjugated to magnetic beads. Detection of CD14 + and AT 1 R expression was achieved by double-labeling flow cytometry. Highly purified monocytes were then stimulated with AngII (6 and 24 h) to assess IL-6 and TNF-a transcript levels by qRT-PCR and protein secretion by ELISA. Results: Monocytes comprised 9.7 ± 2.0% of the PBMCs. Monocyte isolation by negative selection yielded a purity of up to 99.8%. We demonstrated AT 1 R expression on 9.5 ± 0.3% of highly purifed CD14 + /CD16 -monocytes. Stimulation of highly purified monocytes with AngII resulted in increased transcript levels of IL-6 at 6 h but not at 24 h, and increased secretion of IL-6 in a dose-dependent manner compared with controls (p <0.01). Conversely, there was no increase in TNF-a mRNA transcripts or protein secretion. Conclusions: We provide evidence that a CD14 + /CD16 -subset of highly purified human monocytes express AT 1 R and respond to AngII exposure in vitro by producing IL-6 but not TNF-a.

show abstract

“…Tetracyclines, cephalosporins, macrolides, and quinolones have been shown to affect human monocyte responses (3,4,20,(29)(30)(31). Quinolones (ciprofloxacin, ofloxacin, and pefloxacin) have differential effects on LPS-induced cytokine produc- 3.…”

Section: Resultsmentioning

confidence: 99%

“…In effect, a wide range of antimicrobial agents administered in vivo or in vitro have been reported to modify host immune responses (14,17,31), including cytokine production (3,4,20,29,30). …”

mentioning

confidence: 99%

Differential modulation of cytokine production by macrolides: interleukin-6 production is increased by spiramycin and erythromycin

Bailly

Pocidalo

Fay

et al. 1991

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Antibiotics do not act alone but act in conjunction with the host defense system. In pairticular, it has been shown that some antibiotics can modify cytokine production. We compared the in vitro effiects of three macrolides (roxithroinycin, spiramycin, and erythromycin) actively concentrated by leukocytes on interleukinla, (IL-la), IL-1p, , and tumor necrosis factor alpha. production by human monocytes stimulated with lipopolysaccharide. Our results show that the three macrolides tested have different effects on production of these cytokines. Spiramycin and, to a lesser extent, erythromycin increased total IL-6 production without affecting IL-la, IL-1j0, or tumor necrosis factor alpha production, whereas roxithromycin had no effect. To our knowledge, this is the first time that an antibiotic has been shown to increase IL-6 production.Over the last 20 years, a growing interest in the study of interactions between antimicrobial agents and host defense systems has arisen. In effect, a wide range of antimicrobial agents administered in vivo or in vitro have been reported to modify host immune responses (14,17,31), including cytokine production (3,4,20,29,30).Interleukin-la (IL-la), and IL-1p, IL-6, and tumor necrosis factor alpha (TNF-a) are the major cytokines produced by mononuclear phagocytes (6, 10, 23, 34). They mediate a wide range of biological activities that play a critical role in the induction of inflammatory and immune responses. Although these cytokines are biochemically and immunologically distinct proteins transcribed from different genes, they share a number of activities, such as the induction of fever and acute-phase protein synthesis by the liver (5,12,15,26). They have also been found to exhibit synergy, and each can modulate the production of the others (1,25,33).Macrolides are a class of antibiotics taken up and concentrated by leukocytes; as a result, they can reach intracellular concentrations far higher than those attained in the extracellular medium (8,21,28 Cytokine production. Following adherence, the cells were washed, and 1 ml of fresh serum-free medium was added to the monolayers with 10 p,g of lipopolysaccharide (LPS) (Escherichia coli O.55:B5; Difco) per ml and with or without various concentrations of the antibiotics (1, 10, and 50 ,ug/ml). Cultures were incubated for 18 h at 37°C in a humidified atmosphere of 95% air and 5% CO2. To determine extracellular cytokine concentrations, cell-free supernatants were collected, centrifuged at 800 x g for 10 min, and stored at -70°C until assay. To determine cell-associated cytokine concentrations, the cells remaining in the wells were covered with 1 ml of fresh medium, lysed with 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propanesulfonate (CHAPS) (9 mM; Sigma) for 30 min, and stored at -70°C until assay. Addition of CHAPS to any of the recombinant cytokines used in this experiment did not modify the immunoassays.

show abstract

Quinolone-induced differential modification of IL-1α and IL-1β production by LPS-stimulated human monocytes

Cited by 42 publications

References 29 publications

Dysregulation of in vitro cytokine production by monocytes during sepsis.

Dysregulation of in vitro cytokine production by monocytes during sepsis.

Highly purified human peripheral blood monocytes produce IL-6 but not TNFα in response to angiotensin II

Differential modulation of cytokine production by macrolides: interleukin-6 production is increased by spiramycin and erythromycin

Contact Info

Product

Resources

About