Quinolone and Macrolide-ResistantCampylobacter jejuniin Pediatric Gastroenteritis Patients from Central Iran

Abbasi, Elnaz; Belkum, Alex van; Ghaznavi‐Rad, Ehsanollah

doi:10.1089/mdr.2018.0455

Cited by 14 publications

(22 citation statements)

References 39 publications

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“…Table 5. Comparison of the frequency, clinical symptoms, and antibiotic resistance of DEC and E. coli pathotypes in this study and in other studies Conclusion DEC is second only to Campylobacter jejuni in frequency when compared with other enteropathogens in pediatric patients with diarrhea from central Iran, and the abundance is greater for EAEC than for any other pathotype (15). Thus, pediatricians and, even more importantly, experts in laboratory diagnostics should pay special attention to the identification of these E. coli pathotypes.…”

Section: Phenotypic and Genotypic Investigationsupporting

confidence: 49%

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Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran

Abbasi

Mondanizadeh

Belkum

et al. 2020

Preprint

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Background Diarrheagenic Escherichia coli (DEC) is a significant cause of gastroenteritis and a major public health problem. This study investigates the prevalence and the antibiotic resistance patterns of DEC that were isolated from infectious diarrhea samples of pediatric patients from central Iran.Methods Pediatric diarrhea samples were collected from 230 pediatric patients visiting the hospital. E. coli pathotypes were diagnosed by using conventional culture methods and PCR. Antibiotic resistance profiles, the frequency of multi-drug resistance (MDR), and the phenotypic and genotypic characteristics of extended spectrum-β-lactamase (ESBL), AmpC and integron-associated genes were analyzed.Results Of the 230 samples of infectious diarrhea, 91 (39.5%) produced E. coli isolates. Of these, 32 cases (35.1%) were identified as DEC by culture and PCR. The frequency of the E. coli pathotypes obtained was as follows: EAEC 11/32 (34.3%), EPEC 9/32 (28.1%), ETEC 6/32 (18.7%), EIEC 3/32 (9.3%), and EHEC 3/32 (9.3%). The antibiotic resistance rates were greater for nalidixic acid (30/32; 93.7%), ampicillin (29/32; 90.6%), and tetracycline (25/32; 78.1%) than for any of the other tested antibiotics. High levels of MDR (25/32; 78.1%) and the presence of ESBL (18/32; 56.2%) and AmpC (9/32; 28.1%) were observed in the DEC isolates. The isolates showed a higher frequency of the ESBL genes [ bla TEM (18/18; 100%), bla CTX-M15 (17/18; 94.4%)], and AmpC [ bla CIT (4/9; 44.4%) and bla DHA (4/9; 44.4%)] than of the other ESBL and AmpC genes.Conclusion DEC appeared to be the second-most abundant agent of diarrhea in pediatric patients after Campylobacter jejuni , with frequent MDR and ESBL presence.

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Section: Phenotypic and Genotypic Investigationsupporting

confidence: 49%

“…India, DEC was the most common agent found (14)(15)(16). Pediatricians should therefore request standard microbiological methods for determination of the pathogens involved and for designing subsequent therapy.…”

Section: Resultsmentioning

confidence: 99%

Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran

Abbasi

Mondanizadeh

Belkum

et al. 2020

Preprint

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“…Viral gastroenteritis mainly occurs in winter worldwide, with transmission chiefly through the fecal-oral route (10). This report presents for the first time an estimation of the frequency of adenovirus, norovirus, and rotavirus disease in central Iran, and it shows that the prevalence for the three most important species is low in comparison to bacterial disease (11,12). We have demonstrated before that the prevalence of campylobacteriosis and shigellosis in the same period of present study were 19.5% and 8.2%, respectively (11,12).…”

Section: Discussionsupporting

confidence: 63%

“…Adenovirus, norovirus and rotavirus were rarely found as viral agents responsible for gastroenteritis among children in central Iran. Although the frequency of viral gastroenteritis has been low in this area compared to bacterial gastroenteritis (11,12), long-term monitoring of all enteropathogenic agents should be continued in order to screen for the unexpected occurrence of outbreaks and for getting better visibility on the precise seasonality of viral and bacterial diarrhea. Doing so will also help to better understand the precise epidemiology of this serious disease and to help improve appropriate medical diagnosis and management of diarrhea in pediatric patients.…”

Section: Resultsmentioning

confidence: 99%

Low Endemic Frequency of Adenovirus, Rotavirus and Norovirus in Pediatric Diarrhea Samples from Central Iran

Abbasi

Mondanizadeh

Belkum³

et al. 2020

Preprint

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Background: Acute viral gastroenteritis is a disorder that affects children on a worldwide scale but mostly in developing countries. Adenoviruses, rotaviruses, and noroviruses are the major viral cause of childhood gastroenteritis. This study is the first to investigate the prevalence of these viruses in diarrhea samples from pediatric patients living in central Iran.Methods: A total of 173 samples of pediatric diarrhea, from May 2015 to May 2016, was included in this descriptive cross-sectional study. The samples were analyzed using in-house developed PCR and reverse transcription (RT) -PCR methods to investigate the prevalence of adeno-, rota- and noroviruses. Results: Out of 173 samples of pediatric diarrhea, eight were shown to contain enteric viruses (4.6%): four of these were adenovirus (2.3%), three were rotavirus (1.7%), and one represented a genogroup II norovirus (0.6%). Most of the positive samples were obtained from children under the age of seven. The most common additional clinical symptoms in pediatric patients with viral agents were fever, vomiting, and abdominal pain. Conclusions: Adenovirus and rotavirus were rarely found as agents responsible for gastroenteritis in central Iran. Although we show that the frequency of viral gastroenteritis is low in this area compared to bacterial gastroenteritis, still longer-term monitoring of all enteropathogenic agents should provide a deeper understanding of the real endemicity and the possible occurrence of unexpected outbreaks of viral enteritis.

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“…Considering a study which it was done in the past, we showed that DEC (n=32, 13.9%) was second only to Campylobacter jejuni (n=45, 19.5%; p = 0.003) as the most abundant agent of diarrhea in pediatric patients, whereas in Shiraz (Iran) and India, DEC was the most common agent found. [14][15][16] Pediatricians should therefore…”

Section: Resultsmentioning

confidence: 99%

Multi-Drug-Resistant Diarrheagenic Escherichia coli Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran

Abbasi¹,

Mondanizadeh²,

Belkum³

et al. 2020

IDR

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Background: Diarrheagenic Escherichia coli (DEC) is a significant cause of gastroenteritis and a major public health problem. This study investigates the prevalence and the antibiotic resistance patterns of DEC that were isolated from infectious diarrhea samples of pediatric patients from central Iran. Patients and Methods: Pediatric diarrhea samples were collected from 230 pediatric patients visiting the hospital. E. coli pathotypes were diagnosed by using conventional culture methods and PCR. Antibiotic resistance profiles, the frequency of multi-drug resistance (MDR), and the phenotypic and genotypic characteristics of extended spectrum-βlactamase (ESBL), AmpC and integron-associated genes were analyzed. Results: Of the 230 samples of infectious diarrhea, 91 (39.5%) produced E. coli isolates. Of these, 32 cases (35.1%) were identified as DEC by culture and PCR. The frequency of the E. coli pathotypes obtained was as follows: EAEC 11/32 (34.3%), EPEC 9/32 (28.1%), ETEC 6/32 (18.7%), EIEC 3/32 (9.3%), and EHEC 3/32 (9.3%). The antibiotic resistance rates were greater for nalidixic acid (30/32; 93.7%), ampicillin (29/32; 90.6%), and tetracycline (25/32; 78.1%) than for any of the other tested antibiotics. High levels of MDR (25/ 32; 78.1%) and the presence of ESBL (18/32; 56.2%) and AmpC (9/32; 28.1%) were observed in the DEC isolates. The isolates showed a higher frequency of the ESBL genes [bla TEM (18/18; 100%), bla CTX-M15 (17/18; 94.4%)], and AmpC [bla CIT (4/9; 44.4%) and bla DHA (4/9; 44.4%)] than of the other ESBL and AmpC genes. Conclusion: Compared to the previous study, DEC appeared to be the second-most abundant agent of diarrhea in pediatric patients after Campylobacter jejuni, with frequent MDR and ESBL presence.

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Quinolone and Macrolide-ResistantCampylobacter jejuniin Pediatric Gastroenteritis Patients from Central Iran

Cited by 14 publications

References 39 publications

Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran

Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran

Low Endemic Frequency of Adenovirus, Rotavirus and Norovirus in Pediatric Diarrhea Samples from Central Iran

<p>Multi-Drug-Resistant Diarrheagenic <em>Escherichia coli</em> Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran</p>

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