Quinate:NAP(P)+-oxidoreductase from Larix sibirica: purification, characterization and function

Molecular Characterization of Quinate and Shikimate Metabolism in Populus trichocarpa

“…These sequence and structural considerations were in agreement with our hypothesis, and thus in vitro biochemical analyses were warranted DQD/SDHs and QDHs in Poplar-The observed K m (shikimate) values for poplar DQD/SDHs are within the range of other functionally characterized DQD/SDHs (ranging from 130 to 860 M) (14,17,37,54). In contrast, the poplar QDH enzymes characterized here have lower K m (quinate) values (255 and 300 M) compared with previously characterized QDHs partially purified from loblolly pine and Siberian larch, which range from 1.84 to 3.6 mM (36,37). This higher affinity of poplar QDHs toward quinate may indicate highly specialized functions in quinate (derivative) metabolism in poplar.…”

Biosynthetic origin of carbon-based secondary compounds: cause of variable responses of woody plants to fertilization?

“…However, the second major group of phenolics, the hydrolyzable tannins, has gallic acid as its precursor. Two origins for its synthesis have been proposed: either from phenylalanine or from dehydroshikimic acid, an intermediate compound of the shikimate pathway (Ishikura et al 1984;Waterman & Mole 1989;Gross 1992;Ossipov et al 1995) (for the biosynthesis of phenolics in birch, see Fig. 1).…”