Quinacrine and Other Acridines

Wolfe, Alan D.

doi:10.1007/978-3-642-46304-4_14

Cited by 6 publications

(2 citation statements)

References 222 publications

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“…The irreversibility of the bactericidal effect on cells briefly treated with albicidin above the MIC suggested efficient binding of the antibiotic to its target. Because total inhibition of DNA synthesis and partial inhibition of RNA synthesis resulted, either the DNA template itself, or a protein such as DNA gyrase with functions in both DNA replication and transcription are potential targets (Smith & Davies, 1967;White & White, 1967;Wolfe, 1975). It seems possible that albicidin specifically blocks an essential replication protein, and that the apparent partial inhibition of RNA synthesis simply reflected the increasing DNA available for transcription in rapidly growing, untreated control cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Because inhibitors of DNA gyrase and antibiotics binding to DNA can yield similar patterns of inhibition of macromolecule synthesis (Smith & Davies, 1967;White & White, 1967;Wolfe, 1975), we attempted to distinguish between these possible modes of action of albicidin. A total of 24 spontaneous mutants of E. coli UHPl resistant to albicidin, coumermycin A , , novobiocin, or nalidixic acid showed no cross-resistance between albicidin and inhibitors of DNA gyrase.…”

Section: Chemical Characteristics Of Albicidinmentioning

confidence: 99%

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Preliminary Characterization of an Antibiotic Produced by Xanthomonas albilineans Which Inhibits DNA Synthesis in Escherichia coli

Birch

Patil

1985

Microbiology

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Chlorosis-inducing isolates of Xanthomonas albilineans, the sugarcane leaf scald pathogen, produced a mixture of antibacterial compounds in culture. The antibiotic mixture, which eluted as a single strongly retarded peak from Sephadex LH-20 in methanol, was bactericidal to Escherichia coli. Inhibition of E. coli was not reversed by added nutrients, and affected cells were not lysed but many accumulated polyphosphate granules. The major antibacterial component, isolated in crystalline form after HPLC, is given the trivial name albicidin. Near the minimum inhibitory concentration, albicidin caused a complete block to DNA synthesis, followed by partial inhibition of RNA and protein synthesis, as assessed by incorporation of radioactive precursors. Spontaneous antibiotic-resistant mutants of E. coli showed no cross-resistance between albicidin and inhibitors of either subunit of DNA gyrase. Mixing albicidin with purified DNA from E. coli did not alter the thermal denaturation behaviour of the DNA, or the absorption spectrum of the antibiotic. PolA+ and PolA - strains of E. coli were equally sensitive to albicidin, indicating that the antibiotic does not bind to or modify DNA. Selective inhibition of DNA synthesis without evidence of DNA binding suggests a specific interaction of albicidin with an essential replication protein.

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Section: Discussionmentioning

confidence: 99%

Section: Chemical Characteristics Of Albicidinmentioning

confidence: 99%