Quiet breathing in hindlimb casted mice

Receno, Candace N.; Roffo, Katelynn E.; Mickey, Marisa C.; DeRuisseau, Keith C.; DeRuisseau, Lara R.

doi:10.1016/j.resp.2018.06.003

Cited by 2 publications

(4 citation statements)

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“…; Receno et al. ,b) for air breathing, this time span of calm breathing was not observed in these groups of mice, even after multiple chamber habituation days and over 3 h in the chamber during testing. Based on this behavior, we aimed to find a more appropriate way to quantify air breathing that could be used to compare mice in various conditions (e.g., young, old, light, dark).…”

Section: Introductionmentioning

confidence: 72%

“…; Receno et al. ,b). Alternative experimental designs have used baselines where measures are analyzed after a predetermined length of time (DeRuisseau et al.…”

Section: Discussionmentioning

confidence: 98%

“…Whole body unrestrained barometric plethysmography was utilized as previously described (Receno et al. ,b); mice were tested during hours 4–7 of the light cycle and 4–7 of the dark cycle. Briefly, mice were moved to the testing room shortly after calibration of the unrestrained barometric plethysmography set up.…”

Section: Methodsmentioning

confidence: 99%

“…; Receno et al. ,b). In this case, the calm breathing segment used to evaluate the response to gas exposures (hypoxia, hypercapnia, hypoxic hypercapnia) is intended to result in a point by point comparison without the confounds of additional chamber activity.…”

Section: Introductionmentioning

confidence: 98%

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Young and middle‐aged mouse breathing behavior during the light and dark cycles

et al. 2019

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Unrestrained barometric plethysmography is a common method used for characterizing breathing patterns in small animals. One source of variation between unrestrained barometric plethysmography studies is the segment of baseline. Baseline may be analyzed as a predetermined time‐point, or using tailored segments when each animal is visually calm. We compared a quiet, minimally active (no sniffing/grooming) breathing segment to a predetermined time‐point at 1 h for baseline measurements in young and middle‐aged mice during the dark and light cycles. Additionally, we evaluated the magnitude of change for gas challenges based on these two baseline segments. C57 BL /6 JE iJ x C3Sn.BliA‐ Pde6b + /DnJ male mice underwent unrestrained barometric plethysmography with the following baselines used to determine breathing frequency, tidal volume ( VT ) and minute ventilation ( VE ): (1) 30‐sec of quiet breathing and (2) a 10‐min period from 50 to 60 min. Animals were also exposed to 10 min of hypoxic (10% O 2 , balanced N 2 ), hypercapnic (5% CO 2 , balanced air) and hypoxic hypercapnic (10% O 2 , 5% CO 2 , balanced N 2 ) gas. Both frequency and VE were higher during the predetermined 10‐min baseline versus the 30‐sec baseline, while VT was lower ( P < 0.05). However, VE / V O 2 was similar between the baseline time segments ( P > 0.05) in an analysis of one cohort. During baseline, dark cycle testing had increased VT values versus those in the light ( P < 0.05). For gas challenges, both frequency and VE showed higher percent change from the 30‐sec baseline compared to the predetermined 10‐min baseline ( P < 0.05), while VT showed a greater change from the 10‐min baseline ( P < 0.05). Dark cycle hypoxic exposure resulted in larger percent change in breathing frequency versus the light cycle ( P < 0.05). Overall, light and dark cycle pattern of breathing differences emerged along with differences between the 30‐sec behavior observational method versus a predetermined time segment for baseline.

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Section: Introductionmentioning

confidence: 72%

“…; Receno et al. ,b). Alternative experimental designs have used baselines where measures are analyzed after a predetermined length of time (DeRuisseau et al.…”

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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