Quick identification and epidemiological characterization of Francisella tularensis by MALDI-TOF mass spectrometry

López-Ramos, I; Hernández, Marta; Rodrı́guez-Lázaro, David; Gutiérrez, María P.; Zarzosa, Pilar; Orduña, Antonio; March, Gabriel A.

doi:10.1016/j.mimet.2020.106055

Cited by 6 publications

(7 citation statements)

References 29 publications

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“…The scheme provided in Table 4 can be used for the identification of Francisellaceae. According to our data and the literature [13][14][15][16][17][18][19], the 5182-5185 mass of the 50S ribosomal protein L34 was specific for the Francisella genus. Within the Francisella genus, the species F. tularensis could be differentiated from the other Francisella species by the histone-like protein form Beta (Hu-beta) mass (selected biomarker 9449).…”

Section: Francisella Identification By Biomarkersupporting

confidence: 70%

“…The 5182-5185 mass was excluded by BioNumerics as not being (sub)species specific. It is however included as a Francisella genus-specific marker because it was identified as such earlier [13][14][15][16][17][18].…”

Section: Biomarker Selection and Verificationmentioning

confidence: 99%

“…To overcome this, an in-house library was created to distinguish F. tularensis subspecies [13]. Francisella-specific biomarkers were studied for F. tularensis, with and without subspecies differentiation, and for F. philomiragia, F. halioticida and F.salimarina [13][14][15][16][17][18][19]. An in-house database was also described limited to F. tularensis ssp.…”

Section: Introductionmentioning

confidence: 99%

“…holarctica and novicida and F. philomiragia [18] and one limited to different MLVA end PFGE-types of F. tularensis ssp. holarctica from Spain and the Czech Republic [16].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers

et al. 2023

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Francisella tularensis is a zoonotic bacterium that is endemic in large parts of the world. It is absent in the standard library of the most applied matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems: the Vitek MS and the Bruker Biotyper system. The additional Bruker MALDI Biotyper Security library contains F. tularensis without subspecies differentiation. The virulence of F. tularensis differs between the subspecies. The F. tularensis subspecies (ssp.) tularensis is highly pathogenic, whereas the subspecies holarctica displays lower virulence and subspecies novicida and F. tularensis ssp. mediasiatica are hardly virulent. To differentiate the Francisellaceae and the F. tularensis-subspecies, an in-house Francisella library was built with the Bruker Biotyper system and validated together with the existing Bruker databases. In addition, specific biomarkers were defined based on the main spectra of the Francisella strains supplemented with in silico genome data. Our in-house Francisella library accurately differentiates the F. tularensis subspecies and the other Francisellaceae. The biomarkers correctly differentiate the various species within the genus Francisella and the F. tularensis subspecies. These MALDI-TOF MS strategies can successfully be applied in a clinical laboratory setting as a fast and specific method to identify F. tularensis to subspecies level.

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Section: Francisella Identification By Biomarkersupporting

confidence: 70%

Section: Biomarker Selection and Verificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…holarctica and novicida and F. philomiragia [18] and one limited to different MLVA end PFGE-types of F. tularensis ssp. holarctica from Spain and the Czech Republic [16].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers

et al. 2023

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“…Matrix‐assisted laser desorption/ionization‐time‐of‐flight mass spectrometry (MALDI‐TOF MS) is an analytical method for simple, rapid and accurate identification of micro‐organism at the genus level, in many cases at the species level, but in some cases also at the strain level (López‐Ramos et al, 2020; Rahi et al, 2016; Singhal et al, 2015; Tran et al, 2015). MALDI‐TOF MS has been used mainly as a tool for identification of bacterial, fungal and yeast isolates (Pavlovic et al, 2014; Wieme et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Euglena gracilis strains Z and bacillaris by MALDI-TOF MS

Lukáčová

Beck

Trnková

et al. 2022

Journal of Applied Microbiology

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Aims: Euglena gracilis is used as model organism for various microbiological, molecular biological and biotechnological studies. Its most studied wild-type strains are Z and bacillaris, but their discrimination by standard molecular methods is difficult.Therefore, we decided to test the suitability of MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) for identification of E. gracilis and for discrimination of these two strains possessing functional chloroplasts. MALDI-TOF MS profiling was also tested for two white (non-photosynthetic) stable E. gracilis mutant strains W gm ZOflL and W 10 BSmL. Methods and results:We have successfully obtained main spectrum profiles (MSPs) of E. gracilis strains Z, SAG 1224-5/25 and bacillaris, SAG 1224-5/15 using protein extraction procedure. Subsequent MALDI-TOF MS profiling of a number of tested samples and the comparison of the obtained protein profiles with our in-house database including MSPs of both strains have revealed that these two strains can be easily distinguished by MALDI-TOF MS based on score values over two in most cases.This method has also confirmed the ancestry of white mutant strains W gm ZOflL and W 10 BSmL, originally derived from strains Z and bacillaris, respectively.Conclusions: MALDI-TOF MS is suitable, accurate and rapid method for discrimination of E. gracilis strains. Significance and Impact of the Study: These results can have broad practical implications for laboratories cultivating various strains of euglenids, and they can be applied for their discrimination by MALDI-TOF MS.

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Tularemia – a re-emerging disease with growing concern

Sharma,

Patil,

Singh

et al. 2023

Veterinary Quarterly

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Tularemia caused by Gram-negative, coccobacillus bacterium, Francisella tularensis, is a highly infectious zoonotic disease. Human cases have been reported mainly from the United States, Nordic countries like Sweden and Finland, and some European and Asian countries. Naturally, the disease occurs in several vertebrates, particularly lagomorphs. Type A (subspecies tularensis ) is more virulent and causes disease mainly in North America; type B (subspecies holarctica ) is widespread, while subspecies mediasiatica is present in central Asia. F. tularensis is a possible bioweapon due to its lethality, low infectious dosage, and aerosol transmission. Small mammals like rabbits, hares, and muskrats are primary sources of human infections, but true reservoir of F. tularensis is unknown. Vector-borne tularemia primarily involves ticks and mosquitoes. The bacterial subspecies involved and mode of transmission determine the clinical picture. Early signs are flu-like illnesses that may evolve into different clinical forms of tularemia that may or may not include lymphadenopathy. Ulcero-glandular and glandular forms are acquired by arthropod bite or handling of infected animals, oculo-glandular form as a result of conjunctival infection, and oro-pharyngeal form by intake of contaminated food or water. Pulmonary form appears after inhalation of bacteria. Typhoidal form may occur after infection via different routes. Human-to-human transmission has not been known. Diagnosis can be achieved by serology, bacterial culture, and molecular methods. Treatment for tularemia typically entails use of quinolones, tetracyclines, or aminoglycosides. Preventive measures are necessary to avoid infection although difficult to implement. Research is underway for the development of effective live attenuated and subunit vaccines.

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Quick identification and epidemiological characterization of Francisella tularensis by MALDI-TOF mass spectrometry

Cited by 6 publications

References 29 publications

Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers

Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers

Discrimination of Euglena gracilis strains Z and bacillaris by MALDI-TOF MS

Tularemia – a re-emerging disease with growing concern

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