“…The detection of limit (LOD) is calculated to be 1.6 nM based on the criterion of signal-tonoise ratio = 3. Compared with some existing colorimetric methods for ATP detection, the proposed biosensor provided lower detection limit [9,26,31,32]. The results demonstrate that highly sensitive detection of ATP can be realized.…”
Section: Sensitivity Of the Sensormentioning
confidence: 90%
“…Liu et al [8] proposed a homogenous colorimetric sensor for ATP detection based on DNAzyme-aptamer; this method was simple and high effective, but the LOD is not satisfactory. Microporous materials metal-organic frameworks (MOFs) which possess large surface area and high pore volume are applied to fabricate a highly sensitive colorimetric system for ATP assay [9]. Despite its excellent performance, the complicated synthesis is laborious and time-consuming.…”
A facile and economic colorimetric strategy was designed for ATP detection by rationally using urease, a pH-responsive molecule, and a metal-mediated switchable DNA probe. By utilizing metal ions as a modulator of urease activity, the concentration of ATP is translated into pH change, which can be readily visualized by naked eye. An unmodified single-stranded DNA probe was designed, which consists of a target binding sequence and two flanked cytosine (C)-rich sequences. This C-rich singlestranded DNA can form a hairpin structure triggered by Ag + ions via C-Ag +-C base mismatch. Upon introduction of ATP, Ag +coordinated hairpin DNA structure will be broken and release the included Ag + , thus inhibiting the activity of urease. Conversely, urease can hydrolyze urea and raise pH value of the solution, resulting in the color change of the sensing solution. The proposed assay allows determination of ATP as low as 1.6 nM and shows a satisfactory result in human serum. Because of simple operation and low cost of this method, we believe it has a potential in point-of-care (POC) testing in resource-limited areas.
“…The detection of limit (LOD) is calculated to be 1.6 nM based on the criterion of signal-tonoise ratio = 3. Compared with some existing colorimetric methods for ATP detection, the proposed biosensor provided lower detection limit [9,26,31,32]. The results demonstrate that highly sensitive detection of ATP can be realized.…”
Section: Sensitivity Of the Sensormentioning
confidence: 90%
“…Liu et al [8] proposed a homogenous colorimetric sensor for ATP detection based on DNAzyme-aptamer; this method was simple and high effective, but the LOD is not satisfactory. Microporous materials metal-organic frameworks (MOFs) which possess large surface area and high pore volume are applied to fabricate a highly sensitive colorimetric system for ATP assay [9]. Despite its excellent performance, the complicated synthesis is laborious and time-consuming.…”
A facile and economic colorimetric strategy was designed for ATP detection by rationally using urease, a pH-responsive molecule, and a metal-mediated switchable DNA probe. By utilizing metal ions as a modulator of urease activity, the concentration of ATP is translated into pH change, which can be readily visualized by naked eye. An unmodified single-stranded DNA probe was designed, which consists of a target binding sequence and two flanked cytosine (C)-rich sequences. This C-rich singlestranded DNA can form a hairpin structure triggered by Ag + ions via C-Ag +-C base mismatch. Upon introduction of ATP, Ag +coordinated hairpin DNA structure will be broken and release the included Ag + , thus inhibiting the activity of urease. Conversely, urease can hydrolyze urea and raise pH value of the solution, resulting in the color change of the sensing solution. The proposed assay allows determination of ATP as low as 1.6 nM and shows a satisfactory result in human serum. Because of simple operation and low cost of this method, we believe it has a potential in point-of-care (POC) testing in resource-limited areas.
“…[46] Xu et al loaded quercetin for label-free colorimetric ATP sensing at neutral pH. [47] Li et al reported glucose oxidase and horseradish peroxidase encapsulated ZIF-90 for an enzyme-linked reaction to amplify the electrochemical signal of an electrochemical immunosensor. [48] Zhang et al encapsulated lassase for online detection of ATP in rat brain.…”
Section: Atp Responsive Drug Release Behavior In Vitromentioning
Tumor reprogram pathway of mitochondrial metabolism is an emerging approach for malignant tumor treatment, such as triple-negative breast cancer. In this study, a tumor/mitochondria cascaded targeting, adenosine-triphosphate (ATP) responsive nanocarrier of zeolitic imidazolate framework-90 (ZIF-90) for breast cancer combination therapy is reported. Atovaquone (AVO) and hemin are loaded into ZIF-90, then a peptide iRGD with tumor-targeting ability is modified on the ZIF-90 nanoplatform. Hemin can specifically degrade BTB and CNC homology1 (BACH1), resulting in the changes of mitochondrial metabolism, and AVO acts as the inhibitor of the electron transport chain (ETC). The degradation of BACH1 using hemin can effectively improve the anti-tumor efficiency of mitochondrial metabolism inhibitor AVO, by increasing dependency on mitochondrial respiration. This nanoplatform displays both tumor-targeting and mitochondria-targeting capacity with high level of ATP responsive drug release behavior. The specific characteristic of mitochondria-targeting ability of this nanoplatform can increase the accumulation of AVO in the mitochondria, and in turn, can effectively improve the inhibition of the ETC. Both in vitro and in vivo results reveal that this composite nanocarrier has excellent tumor inhibition ability with limited side effects. Accordingly, this study provides an attractive strategy in the mitochondrial metabolism for cancer targeted therapy.
“…5−10 MoS 2 , a typical 2D transition metal dichalcogenide, possesses a graphene-like structure and properties and exhibits intrinsic peroxidase-like activity, catalase-like activity, oxidase-like activity, and superoxide dismutase-like activity. 11−16 Some studies have found the enzymatic function of the MoS 2 nanozyme could be inhibited by the introduction of biological materials such as L-cysteine, 17 glutathione, 18 quercetin, 19 and lipase, 20 and the inhibitors were quantitatively detected based on the concentration-dependent inhibition manner.…”
In this paper, we presented an ATP detection platform based on the selective activation of an artificial nanozyme inhibited by ZIF-90 nanoparticles. For the first time, we found and reported the inhibitory effect of ZIF-90 nanoparticles on the MoS 2 nanozyme, and this property was integrated with its ATP-responsive ability to develop an ATP detection biosensor. The presence of ATP triggers the decomposition of ZIF-90, thus eliminating the inhibition ability and recovering the catalytic ability of the MoS 2 nanozyme. By measuring the catalytic activity in the H 2 O 2 −TMB reaction system with optical and electrochemical methods, the ATP detection performance was evaluated systematically. The proposed detection method exhibited excellent sensitivity and selectivity, and the optical and electrochemical modes satisfied the requirements of different application circumstances. In addition, taking advantage of the mild and rapid bacterial disintegration properties of antibiotic peptide-modified magnetic nanoparticles (M-AMPs), using the proposed platform based on selective activation of ZIF-90 nanoparticles inhibited the MoS 2 nanozyme, discriminative ATP detection was performed. This work confirms that the inhibitory effect of ZIF-90 nanoparticles on the MoS 2 artificial nanozyme provides an idea for developing biosensing systems based on enzyme inhibition and, more importantly, holds great potential for expanding the application of ZIF-90 nanoparticles in analytical fields.
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