Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation

Li, Gen; Shen, Xue; Wei, Ying; Si, Xiaosa; Deng, Xuming; Wang, Jianfeng

doi:10.1016/j.intimp.2019.01.017

Cited by 30 publications

(20 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have already demonstrated that ExoS can cause an in ammatory response and improve clearance in the lungs in vitro and vivo [26]. It is also well known that quercetin is a useful substance for various diseases [27][28][29]. However, it is not known whether quercetin prevents disease by inhibiting the expression of ExoS due to infection of the host via T3SS of P. aeruginosa.…”

Section: Discussionmentioning

confidence: 99%

Quercetin attenuates the production of pro-inflammatory cytokines in H292 human lung epithelial cells infected with Pseudomonas aeruginosa, by modulating ExoS production

Ahn

Park

Jang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The type three secretion system (T3SS) is a major virulence system of Pseudomonas aeruginosa (P. aeruginosa). The effector protein Exotoxin S (ExoS) produced by P. aeruginosa is secreted into the host cells via the T3SS. For the purpose of screening the inhibitors with regard to ExoS secretion, we developed the sandwich-type enzyme-linked immunosorbent assay (ELISA) system. From the initial screening, quercetin was selected because it has the prominent effect of ExoS inhibition and also is known to have anti-inflammatory and antioxidant effects on mammalian cells.Results: In this study, we investigated the effects of quercetin on the expression and secretion of ExoS using the ELISA and Western blot analysis methods. The results showed that the secretion of ExoS was significantly decreased by 10, 20uM quercetin. Also, pscF and popD, which are composed of the T3SS needle, are reduced by quercetin at the mRNA level, and we confirmed the inhibitory effect of quercetin on cytokines in P. aeruginosa-infected H292 cells by real-time polymerase chain reaction (PCR). Conclusion: Collectively, quercetin inhibits the secretion of ExoS by reducing both ExoS production and the expression of the needle protein of T3SS. Furthermore, these results suggest that quercetin has the potential to be used as an anti-toxic treatment for the disease caused by P. aeruginosa infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Quercetin attenuates the production of pro-inflammatory cytokines in H292 human lung epithelial cells infected with Pseudomonas aeruginosa, by modulating ExoS production

Ahn

Park

Jang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Such an anti-inflammatory effect could also be seen in vivo upon pulmonary infection of emphysema mice, although the quercetin impact on NTHi bacterial counts was only moderate in this experimental setting. In vivo treatment of mouse infection with quercetin, by using regimens ranging from 25 to 150 mg/kg, has reported fairly variable results, from Streptococcus suis virulence reduction and suppression of lethal shock upon Salmonella typhimurium infection [55,67], to a moderate decrease in Helicobacter pylori counts [68], no effect on bacterial counts in a mouse model of periodontitis by Aggregatibacter actinomycetemcomitants [69], or even increased Chlamydia pneumoniae counts in murine lungs [70]. Here, quercetin administration showed a non-significant trend towards reducing NTHi bacterial counts in mouse lung homogeneates, and a clearer protective effect on the zebrafish sepsis model system.…”

Section: Discussionmentioning

confidence: 99%

“…Quercetin has shown to reduce proinflammatory cytokine production in Vibrio cholerae infected intestinal epithelial cells [54], and in Streptococcus suis infected macrophages [55]. To determine if quercetin modulates the expression of genes encoding proinflammatory cytokines in human airway epithelial cells followed by NTHi infection, A549 cells were pretreated with quercetin.…”

Section: Quercetin Lowers Proinflammatory Gene Expression By Epithelimentioning

confidence: 99%

Preclinical Evaluation of the Antimicrobial-Immunomodulatory Dual Action of Xenohormetic Molecules against Haemophilus influenzae Respiratory Infection

et al. 2019

View full text Add to dashboard Cite

Chronic obstructive pulmonary disease (COPD) is characterized by abnormal inflammation and impaired airway immunity, providing an opportunistic platform for nontypeable Haemophilus influenzae (NTHi) infection. In this context, therapies targeting not only overactive inflammation without significant adverse effects, but also infection are of interest. Increasing evidence suggests that polyphenols, plant secondary metabolites with anti-inflammatory and antimicrobial properties, may be protective. Here, a Cistus salviifolius plant extract containing quercetin, myricetin, and punicalagin was shown to reduce NTHi viability. Analysis of these polyphenols revealed that quercetin has a bactericidal effect on NTHi, does not display synergies, and that bacteria do not seem to develop resistance. Moreover, quercetin lowered NTHi airway epithelial invasion through a mechanism likely involving inhibition of Akt phosphorylation, and reduced the expression of bacterially-induced proinflammatory markers il-8, cxcl-1, il-6, pde4b, and tnfα. We further tested quercetin’s effect on NTHi murine pulmonary infection, showing a moderate reduction in bacterial counts and significantly reduced expression of proinflammatory genes, compared to untreated mice. Quercetin administration during NTHi infection on a zebrafish septicemia infection model system showed a bacterial clearing effect without signs of host toxicity. In conclusion, this study highlights the therapeutic potential of the xenohormetic molecule quercetin against NTHi infection.

show abstract

“…QUE is an abundant nature flavonoid which could be found in many fruits, vegetables, leaves and grains. It has been reported that QUE has the biological functions of anti-inflammation, anti-ischemia, antioxidation and anti-tumor [11][12][13][14]. Also, QUE is a strong antioxidant and radical scavenger [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Quercetin reduces tendon adhesion in rat through suppression of oxidative stress

Liang

Zhang

et al. 2020

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Background Tendon adhesion is one of the most common clinical problems, which poses a considerable challenge to orthopedics doctors. Quercetin (QUE) as a popular drug at present, it has various biological functions, including anti-inflammatory, anti-ischemic, anti-peroxidation, and antioxidant. The purpose of this study was to investigate the effect of quercetin on tendon adhesion and whether quercetin can inhibit oxidative stress. Method Thirty-six rats were randomly divided into three groups, including control group, low QUE (50 mg/kg/day) group, and high QUE (100 mg/kg/day) group. After 1 week, the levels of SOD, MDA and GPx were measured. The degree of tendon adhesion was assessed by macroscopic evaluation and histological evaluation. After 4 weeks. Besides, the pharmacological toxicity of quercetin to main organs were evaluated by histological analysis. Results The extent of superoxide dismutase (SOD) and glutathione peroxidase (GPx) of tendon tissue in high QUE group was significantly higher than those of low QUE group and control group. And the extent of malondialdehyde (MDA) of tendon tissue in high QUE group was significantly lower than that of low QUE group and control group. By macroscopic evaluation and histological analysis, the extent of tendon adhesion in high QUE group was lower than low QUE group and control group. However, there were no significant changes of the major organs through histological analysis. Conclusions Quercetin may be a good and safe strategy in preventing tendon adhesion. But further clinical research is needed before its recommendation in the prevention and treatment of tendon adhesion.

show abstract

Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation

Cited by 30 publications

References 39 publications

Quercetin attenuates the production of pro-inflammatory cytokines in H292 human lung epithelial cells infected with Pseudomonas aeruginosa, by modulating ExoS production

Quercetin attenuates the production of pro-inflammatory cytokines in H292 human lung epithelial cells infected with Pseudomonas aeruginosa, by modulating ExoS production

Preclinical Evaluation of the Antimicrobial-Immunomodulatory Dual Action of Xenohormetic Molecules against Haemophilus influenzae Respiratory Infection

Quercetin reduces tendon adhesion in rat through suppression of oxidative stress

Contact Info

Product

Resources

About