Quercetin Reduces Inflammatory Pain: Inhibition of Oxidative Stress and Cytokine Production

Valério, Daniel Augusto Rodrigues; Georgetti, Sandra R.; Magro, Danilo A.; Casagrande, Rúbia; Cunha, Thiago M.; Vicentini, Fabiana T. M. C.; Vieira, Sílvio M.; Fonseca, Maria José Vieira; Ferreira, Sérgio Henrique; Cunha, Fernando Q.; Verri, Waldiceu A.

doi:10.1021/np900259y

Cited by 151 publications

(124 citation statements)

References 31 publications

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“…Our results demonstrate that quercetin mitigates the expression levels of genes associated with inflammation, including IL-1β and TNF. Those results are consistent with the report that quercetin lowers the level of proinflammatory gene expression as a consequence of oxidative stress and different stimuli (Chuammitri et al, 2015;Valerio et al, 2009). Accordingly, the antiinflammatory action of quercetin on TNF-α is dosedependent manner (Nair et al, 2006).…”

Section: Discussionsupporting

confidence: 92%

“…In fact, quercetin affects the mRNA gene expressions and associated proteins in the innate immune cells, such as the reduction of the IL-1β mRNA level during inflammation was documented (Valerio et al, 2009). We have previously demonstrated the in vitro effects of quercetin on bovine neutrophil phagocytosis of Escherichia coli (Chuammitri et al, 2015), but in great detail study of how gene cascades in bovine neutrophil phagocytic processes were regulated due to proposed quercetin effects is incompletely elucidated at molecular levels.…”

Section: Introductionmentioning

confidence: 99%

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Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Srikok

Nambut

Wongsawan

et al. 2017

American Journal of Animal and Veterinary Sciences

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This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions of CORO1A, CYBA (gp91 phox ), LAMP1, RAB7A, RAC1 and PAK1 mRNA were analyzed by real-time PCR. In the time-course experiment, treated neutrophils were allowed to co-cultured with live bacteria for 30, 60 and 90 min before measuring gene expressions. The expression levels of IL-1 β and TNF genes in order to demonstrate the antiinflammatory property of quercetin were assessed by conventional RT-PCR. The results of flow cytometry and microscopic examination showed that the percentage of neutrophils performing phagocytosis were significantly higher in QH group in comparison with other groups. We reported here that mRNA expressions of CORO1A, CYBA, LAMP1, RAB7A, RAC1 and PAK1 genes involved in phagocytosis, were significantly up-regulated in QH group. As expected, CytB group had profound down-regulation of genes with one exception in PAK1. From our observations, the expression levels of phagocytic process genes in the QH group were optimum at 60 min and started to decline at 90 min of incubation. The data also indicated that quercetin inhibited inflammation by reducing the expressions of both IL-1β and TNF. In conclusion, the information in our experiments conclude that quercetin has the ability to boost the expression of genes involved in phagocytosis while reduces the expression of proinflammatory genes.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Srikok

Nambut

Wongsawan

et al. 2017

American Journal of Animal and Veterinary Sciences

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“…In agreement, many species of Tephrosia genus present flavonoids (Arriaga et al, 2009a;Arroio et al, 2004;Jang et al, 2003). There is evidence that isolated flavonoids reduce mechanical and thermal hyperalgesia (e.g., increased response to nociceptive stimuli; pain) by activating the endogenous opioid system (Anjaneyulu & Chopra, 2003;Delporte et al, 2007;Loscalzo et al, 2008Loscalzo et al, , 2011 and inhibition of hyperalgesic cytokines production (Chou et al, 2003;Kandhare et al, 2012;Valério et al, 2009b;Valsecchi et al, 2008).…”

Section: Introductionmentioning

confidence: 72%

“…Flavonoids are phenolic compounds with analgesic activity (Anjaneyulu & Chopra, 2003;Chou et al, 2003;Delporte et al, 2007;Kandhare et al, 2012;Loscalzo et al, 2008Loscalzo et al, , 2011Valério et al, 2009b;Valsecchi et al, 2008). Preliminary results detected the presence of flavonoids including novel compounds in the Tephrosia sinapou ethyl acetate extract and others have also shown the presence of flavonoids in Tephrosia genus plants (Arriaga et al, 2009a;Arroio et al, 2004;Jang et al, 2003).…”

Section: Discussionmentioning

confidence: 88%

Tephrosia sinapouethyl acetate extract inhibits inflammatory pain in mice: Opioid receptor dependent inhibition of TNFαand IL-1βproduction

Martínez¹,

Zarpelon²,

Cardoso³

et al. 2013

Pharmaceutical Biology

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Context: Tephrosia toxicaria is currently known as Tephrosia sinapou (Buc'hoz) A. Chev. (Fabaceae) and is a source of compounds such as flavonoids that inhibit inflammatory pain. Objective: To investigate the analgesic effect and mechanisms of the ethyl acetate extract of T. sinapou in inflammatory pain in mice. Materials and methods: Behavioral responses were evaluated using mechanical (1-24 h) and thermal hyperalgesia (0.5-5 h), writhing response (20 min) and rota-rod (1-5 h) tests. Neutrophil recruitment (myeloperoxidase activity), cytokines (tumor necrosis factor [TNF]a and interleukin [IL]-1b), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were determined by colorimetric assays. Pharmacological treatments were opioid receptor antagonist (naloxone, 0.1-1 mg/kg) and control opioid (morphine, 5 mg/kg). Inflammatory stimuli were carrageenin (100 mg/paw), complete Freund's adjuvant (CFA, 10 ml/paw), prostaglandin E 2 (PGE 2 , 100 ng/paw) and acetic acid (0.8%). Results: The intraperitoneal pre-treatment with extract inhibited in a dose-dependent (30-300 mg/kg) dependent manner the mechanical hyperalgesia induced by carrageenin (up to 93% inhibition). The post-treatment (100 mg/kg) inhibited CFA-induced hyperalgesia (up to 63% inhibition). Naloxone (1 mg/kg) prevented the inhibitory effect of the extract over carrageenin-induced mechanical (100%) and thermal (100%) hyperalgesia, neutrophil recruitment (52%) and TNFa (63%) and IL-1b (98%) production, thermal threshold in naïve mice (99%), PGE 2 -induced mechanical hyperalgesia (88%) and acetic acid-induced writhing response (49%). There was no significant alteration in the rota-rod test, and AST and ALT serum levels by extract treatment. Discussion and conclusion: Tephrosia sinapou ethyl acetate extract reduces inflammatory pain by activating an opioid receptor-dependent mechanism.

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“…Flavonoids, saponins and tannins have been shown to exert analgesic effect on acetic acid induced writhing test (Calixto et al, 2000). Previous reports also suggest that catechins, quercetin may exhibit anti-nociceptive and antiinflammatory activity by inhibiting the pronociceptive cytokine production (e.g., TNF-α and IL-1β) and the oxidative imbalance mediated inflammatory pain (Maroon et al, 2010;Valerio et al, 2009). Hence, the flavonoids and tannins present in the extract might be responsible partly for the observed analgesic and antiinflammatory effects.…”

Section: Discussionmentioning

confidence: 98%

Anti-Inflammatory, Analgesic and Antioxidant Activities of <i>Allophylus Cobbe</i> Leaves

Jain

Hossain²,

Fatema³

et al. 2014

American Journal of Pharmacology and Toxicology

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Allophylus cobbe L. Raeuschel (Family-Sapindaceae) is a medicinal plant used traditionally for the treatment of various health risks like pain, inflammation, ulcers and wounds in Bangladesh. This study determined the polyphenolic compounds and evaluated the analgesic, anti-inflammatory and antioxidant effects of the ethanol extract of Allophylus cobbe leaves. High Performance Liquid Chromatography (HPLC) analysis was used to determine the polyphenolic compounds present in the extract. The analgesic activity was evaluated by hot plate and acetic acid induced writhing in mice at two different doses of 250 and 500 mg kg −1 body weight. The extract was also investigated for the anti-inflammatory effect on rats at above mentioned doses using carrageenan induced rat paw edema method. The antioxidant potential of the extract was determined in terms of radical scavenging ability of the stable 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH) free radical. Preliminary phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins and glycosides in the extract. High Performance Liquid Chromatography (HPLC) analysis also confirmed the presence of polyphenolic compounds such as (+)-catechin hydrate, (-)-epicatechin, caffeic acid, p-coumaric acid and quercetin. The extract increased the licking time of hind paw when placed on a hot plate by 35.31% at 250 mg kg −1 dose which is comparable to the increase shown by diclofenac sodium (42.73%) at the 3rd h of study in hot plate test. Moreover, the extract also showed good analgesic effect in acetic acid induced writhing test. The percent inhibition of writhing response by the extract was 85.96 and 78.07% at 250 and 500 mg kg −1 doses respectively while that of the standard drug was 66.67%. Furthermore, the extract also reduced carrageenan induced paw edema formation; the most prominent inhibition was found to be 58.88% (250 mg kg −1 ) at the 3rd h of study. The DPPH radical scavenging activity of the extract increased markedly with increasing concentrations. At a concentration of 200 µg mL −1 , the scavenging activity of the ethanol extract (91.53% inhibition) was comparable to that of the standard ascorbic acid (99.3% inhibition). Our results suggest that Allophylus cobbe extract possesses significant antinociceptive, anti-inflammatory and free radical scavenging activities which may justify the folkloric use of the plant in several communities for conditions such as colic, fever, inflammatory rheumatic pains and other oxidative stress associated disorders.

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Quercetin Reduces Inflammatory Pain: Inhibition of Oxidative Stress and Cytokine Production

Cited by 151 publications

References 31 publications

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Tephrosia sinapouethyl acetate extract inhibits inflammatory pain in mice: Opioid receptor dependent inhibition of TNFαand IL-1βproduction

Anti-Inflammatory, Analgesic and Antioxidant Activities of <i>Allophylus Cobbe</i> Leaves

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