Quercetin reduces adhesion and inhibits biofilm development by Listeria monocytogenes by reducing the amount of extracellular proteins

Vázquez-Armenta, F.J.; Bernal-Mercado, Ariadna Thalía; Tapia-Rodríguez, Melvin R.; González‐Aguilar, Gustavo A.; López-Zavala, Alonso A.; Martínez-Téllez, Miguel Ángel; Hernández‐Oñate, Miguel Ángel; Ayala-Zavala, J. Fernando

doi:10.1016/j.foodcont.2018.02.041

Cited by 53 publications

(28 citation statements)

References 51 publications

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“…Kim et al [ 37 ] found that a quercetin-pivaloxymethyl conjugate (Q-POM) at 5 μ g/mL inhibited 70% of biofilm establishment by a vancomycin-resistant E. faecium isolate. Vazquez-Armenta et al [ 38 ] found that quercetin would hinder the abiotic surface colonisation of Listeria monocytogenes at concentrations below the MIC. In addition, Lee et al [ 39 ] obtained that quercetin has an inhibitory effect on genes related to bacterial adhesion.…”

Section: The Main Pharmacological Activity Of Quercetinmentioning

confidence: 99%

Quercetin: Its Main Pharmacological Activity and Potential Application in Clinical Medicine

Yang

Wang

Long

et al. 2020

Oxidative Medicine and Cellular Longevity

293

148

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Quercetin is a flavonoid compound widely present in plants and exhibits a variety of biological activities. Research on quercetin has shown its potential for medical application. In this research, we elucidate its antioxidant mechanism and the broad-spectrum antibacterial and antiparasite properties; summarise its potential application in antioncology and cardiovascular protection and anti-immunosuppression treatment; and demonstrate its ability to alleviate the toxicity of mycotoxins. This research is expected to offer some insights and inspirations for the further study of quercetin, its properties, and the scientific basis for its better application in clinical practice.

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Section: The Main Pharmacological Activity Of Quercetinmentioning

confidence: 99%

Quercetin: Its Main Pharmacological Activity and Potential Application in Clinical Medicine

Yang

Wang

Long

et al. 2020

Oxidative Medicine and Cellular Longevity

293

148

View full text Add to dashboard Cite

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“…In particular, L. monocytogenes is a concern, especially in the food industry, due to the capacity to form a resistant biofilm [ 1 ]. For its formation, L. monocytogenes, first, from the planktonic state, gets reversibly attached to the surface, then, the subpopulation of the bacteria irreversibly remains on the surface tightly adhered to by the extracellular excreted polymers (e.g., proteins and polysaccharides); then, microcolonies form and further configure a multilayer biofilm [ 1 , 23 , 78 , 79 ]. Therefore, blocking the initial reversive steps is crucial to avoid biofilm formation [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

“…PCs own the ability to inhibit those mechanisms and disrupt the preformed biofilm. In this sense, quercetin, a flavonol with known ABA [ 9 ], produced the inhibition of L. monocytogenes biofilm formation [ 78 ]. These authors indicated that at lower concentrations (60 µg/mL), the main mechanisms of inhibition were focused on reducing the reversible attachment, the amount of the extracellular proteins, and the organization of the bacterial colonies.…”

Section: Discussionmentioning

confidence: 99%

Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity

et al. 2020

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Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives. The objectives of this study were to assess the phytochemical composition by high-performance liquid chromatography–quadrupole time-of-flight mass/mass spectrometry (HPLC-qTOF-MS/MS), total phenolic content (TPC), proanthocyanidin (PAC) content, and antioxidant activity of the APE, the organic fraction (OF), the aqueous fraction (AF), and the acid-microwave hydrolyzed APE (HAPE), on the antibacterial activity (ABA). The results indicated that APE and OF contained (p ˂ 0.05) a higher phenolic composition and antioxidant activity than AF and HAPE. The ABA specified that Pseudomonas aeruginosa and Bacillus cereus were inhibited by all the extracts (minimal inhibitory concentration—MIC ≥ 500 µg/mL), Staphylococcus aureus was only significantly inhibited by APE (≥750 µg/mL), the same MIC was observed for the OF on Salmonella spp. and Listeria monocytogenes. The HAPE increased the inhibitory efficiency up to 25% on Escherichia coli and Salmonella spp. (MIC ≥ 750 µg/mL), and 83.34% on L. monocytogenes (MIC ≥ 125 µg/mL) compared to APE (MIC ≥ 750 µg/mL). Also, HAPE inhibited the biofilm formation at the lowest concentration (125 µg/mL); meanwhile, the biofilm disruption showed to be concentration-time-dependent (p ˃ 0.05) compared to amoxicillin. In conclusion, the fractionation and hydrolyzation of APE improved the ABA; thus, those strategies are useful to design new antimicrobial compounds.

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“…On the contrary, Tunisian propolis inhibited the oral biofilm at a concentration of higher than MIC compared to other studies [25]. According to reports, Quercetin inhibited the anti-biofilm activity against bacterial pathogens such as Pseudomonas aeruginosa [16, 17], Staphylococcus aureus [18] , Streptococci mutans [26] and Listeria monocytogenes [19] indicating that Q had a potential of controlling the biofilm formation and may inhibit biofilm through specific mechanisms such as the initial adhesion or production of extracellular polymeric substances [19]. Therefore, the biofilm inhibitory effect of Q in the present study might be through similar mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Quercetin has a wide range of pharmacological properties such as the anti-inflammation and anti-oxidation; hence, it can be beneficial to the human health. Previous studies on bioactive effects of quercetin revealed that it could inhibit the growth of different microorganisms, especially gram-positive bacteria ( Bacillus subtilis , Micrococcus luteus , Staphylococcus aureus and Staphylococcus epidermidis) and gram negative bacilli ( Escherichia coli and Pseudomonas aeruginosa ) [16–19]. Quercetin applied anti-proliferative and antitumor activities via various mechanisms such as cell cycle arrest during G0/G1 or G2/M phases of the cell cycle in leukemia, breast carcinoma and esophageal adenocarcinoma cells [20–22].…”

Section: Introductionmentioning

confidence: 99%

Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines

Asgharpour

Moghadamnia

Zabihi

et al. 2019

BMC Complement Altern Med

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Background Propolis is a natural bee product with a wide range of biological activities that are related to its chemical composition. The present study investigated the quantification of quercetin (Q) in Ardabil ethanol extract of propolis (AEEP), and then compared its anti-bacterial, anti- biofilm and cytotoxic effects on cancer and normal cell lines. Method In the present study, the chemical composition of AEEP was determined through the high-performance liquid chromatography (HPLC). The AEEP and its main component, quercetin (Q), were evaluated in vitro against 57 oral streptococci by a broth micro-dilution method. The biofilm formation was assessed through the crystal violet staining and MTT assays. The impact of AEEP and Q anti-proliferative effect were evaluated on the fibroblast as normal and cancer cell lines (KB and A431). Results The Q concentration in the composition of AEEP was 6.9% of all its components. The findings indicated that the AEEP and Q were efficient against the cariogenic bacteria and were able to inhibit the S.mutans biofilm adherence at a sub-MIC concentration. Moreover, electron micrographs indicated the inhibition of biofilms compared to control biofilms. In addition, the AEEP and Q indicated a dose-dependent cytotoxic effect on A431 and KB cell lines. On the contrary, they had no cytotoxic effect on fibroblast cells. Conclusion The results indicated that the synergistic impact of main components of AEEP was related to the inhibition of the cancer cell proliferation, cariogenic bacteria and oral biofilm formation. It may play a promising role in the complementary medicine and, it is suggested to be used as food additives.

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Quercetin reduces adhesion and inhibits biofilm development by Listeria monocytogenes by reducing the amount of extracellular proteins

Cited by 53 publications

References 51 publications

Quercetin: Its Main Pharmacological Activity and Potential Application in Clinical Medicine

Quercetin: Its Main Pharmacological Activity and Potential Application in Clinical Medicine

Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity

Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines

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