Quercetin quantification in a Jeju Dendropanax morbifera Lev. extract by varying different parts, harvest times, and extraction solvents

Abstract

Dendropanax morbifera Leveille (Araliaceae) is an endemic species growing in the south-western part of South Korea that has been used in folk medicine and health functional food. In this study, we investigated an extract of quercetin in Jeju D. morbifera by varying different parts (fruit, sprouts, leaves, sprigs, and branches), harvest times, and extraction solvents. In addition, we aimed to establish a simple and reliable HPLC/UV analytical method to determination of quercetin for the quality control and base line data of the Jeju D. morbifera extract as a health functional food ingredient. The analytical specificity was determined with retention time and photo diode array (PDA) spectrum by analyzing quercetin using HPLC and comparing the results to those of extracts. This analytical method for quercetin was validated for its limit of detection (LOD), limit of quantitation (LOQ), precision, and accuracy. A high linearity in the standard calibration curve was obtained, with a coefficient of determination (R 2 ) of 0.9996. Also, the LOD and LOQ values were found to be 0.28 μg/mL and 0.85 μg/mL, respectively, and the recoveries of quantified compounds ranged from 97.91% to 104.10%. Furthermore, the relative standard deviation (RSD) values of data from the intra-and inter-day precision analyses were less than 1.36% and 3.65%, respectively. As a result, the highest quercetin content among the extracts of Jeju D. morbifera leaves was found to be 20.14 mg/g, which was extracted at harvest in May (cultivation period 10 years) with 60% EtOH. All in all, we believe that the results obtained would be helpful in the development of nutraceutics and natural medicines and for the quality control of D. morbifera.

Endothelium-dependent and endothelium-independent vasorelaxant effects of unripe Rubus coreanus Miq. and Dendropanax morbiferus H. Lév. extracts on rat aortic rings

“…Recently, a report demonstrated that DP contains phenolic compounds, including rutin, chlorogenic acid, (+)-catechin, ferulic acid, myricetin, quercetin, and resveratrol, that have anti-inflammatory effects [15]. In our previous study, we suggested quercetin as a marker compound of D. morbiferus after analyzing plant parts (fruits, sprouts, leaves, sprigs, and branches), harvest times, and extraction solvents for quality control [16]. In another study, we reported that L -arginine and γ-aminobutyric acid (GABA) levels in DP were increased by enzymatic hydrolysis and extraction (producing Hy-DP) [17].…”

Laxative Effects of a Standardized Extract of Dendropanax morbiferus H. Léveille Leaves on Experimental Constipation in Rats

“…DPL was fractionated to n-hexane, chloroform (CHCl 3 ), ethyl acetate (EtOAc), nbutanol (n-BuOH), and water fractions (Supplemental Figure S1). The amounts of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, quercetin, and kaempferol in DPL were compared with the high-performance liquid chromatography (HPLC) method according to our previously reported standard method [11,15]. The samples were dissolved in 50% methanol, sonicated for 20 min, and then analyzed using an Agilent 1260 HPLC system (Agilent Technologies, Palo Alto, CA, USA) equipped with an Eclipse XDB-C 18 column (4.6 × 250 mm, 5 µm).…”

“…leaf extract (DPL) have various pharmacological properties [8][9][10]. Previously, we proposed the use of quercetin and kaempferol as chemical markers for the quality control of D. morbiferus after analyzing parts, seasons, and extraction methods [11]. Moreover, we reported the antiobesity effect [12] and antihypertensive effect [13,14] of DPL.…”

Combined antihypertensive effect of unripe Rubus coreanus Miq. and Dendropanax morbiferus H. Lév. Extracts in 1 kidney-1 clip hypertensive rats and spontaneously hypertensive rats

“…Active chemicals in D. morbiferus leaf extract (DP) have also various biological effects [8][9][10][11]. Furthermore, we reported the change in the content of quercetin, a marker chemical, depending on the parts, harvest season, and extraction methods of D. morbiferus for quality control [12]. Quercetin and its metabolites, such as 3-(3-hydroxyphenyl)propionic acid (3HPPA), 3,4-dihydroxyphenylacetic acid (DHPA) and 4-methylcatechol (4MC), administered orally are well known to be able to decrease arterial blood pressure [13][14][15].…”