Quercetin Prevents Lipopolysaccharide-Induced Experimental Preterm Labor in Mice and Increases Offspring Survival Rate

Lin, Xinxiu; Peng, Qiaozhen; Zhang, Jiejie; Li, Xun; Huang, Ju; Duan, Si; Zhang, Weishe

doi:10.1007/s43032-019-00034-3

Cited by 8 publications

(6 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Palombo et al (2019) confirmed that luteolin could inhibit STAT3 activity in mice to counteract the proliferative effect of the IL‐22/IL‐6 signaling pathway and could be used as a drug candidate for the treatment of inflammation and proliferative diseases. Lin et al (2020) confirmed that quercetin could inhibit the expression of inflammatory factors induced by LPS through mouse experiments, such as cyclooxygenase‐2 (PTGS2) and IL6. Glinsky (2020) found that quercetin, luteolin, and eriodictyol were structurally similar and could be developed as inhibitors for SARS‐CoV‐2 infection through drug docking screening and gene expression profile analysis.…”

Section: Discussionmentioning

confidence: 97%

Exploring the treatment of COVID‐19 with Yinqiao powder based on network pharmacology

Lin

Wang

Liu

et al. 2021

Phytotherapy Research

View full text Add to dashboard Cite

Coronavirus disease 2019 (COVID‐19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). In China, Yinqiao powder is widely used to prevent and treat COVID‐19 patients with Weifen syndrome. In this study, the screening and verification of active ingredients, target selection and DisGeNET scoring, drug–ingredient–gene network construction, protein–protein interaction network construction, molecular docking and surface plasmon resonance (SPR) analysis, gene ontology (GO) functional analysis, gene tissue analysis, and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to explore the active ingredients, targets, and potential mechanisms of Yinqiao powder in the treatment of COVID‐19. We also predicted the therapeutic effect of Yinqiao powder using TCM anti‐COVID‐19 (TCMATCOV). Yinqiao powder has a certain therapeutic effect on COVID‐19, with an intervention score of 20.16. Hesperetin, eriodictyol, luteolin, quercetin, and naringenin were the potentially effective active ingredients against COVID‐19. The hub‐proteins were interleukin‐6 (IL‐6), mitogen‐activated protein kinase 3 (MAPK3), tumor necrosis factor (TNF), and tumor protein P53 (TP53). The potential mechanisms of Yinqiao powder in the treatment of COVID‐19 are the TNF signaling pathway, T‐cell receptor signaling pathway, Toll‐like receptor signaling pathway, and MAPK signaling pathway. This study provides a new perspective for discovering potential drugs and mechanisms of COVID‐19.

show abstract

Section: Discussionmentioning

confidence: 97%

Exploring the treatment of COVID‐19 with Yinqiao powder based on network pharmacology

Lin

Wang

Liu

et al. 2021

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…To address the infection related PTL, several mouse models have been developed using bacteria directly ( E. coli ) or LPS, the toxic component on the surface of gram‐negative bacteria. LPS induces PTL and preterm birth (PTB) 41–48 …”

Section: Discussionmentioning

confidence: 99%

“…In particular, M1 macrophages produce proinflammatory cytokines LPS induces PTL and preterm birth (PTB). [41][42][43][44][45][46][47][48] Treatment of mice with LPS is widely used to mimic human PTL. Thus, in this study, we used bacterial LPS to induce PTL in an established model that mimics infection; whether this also replicates other inflammatory events that lead to PTL remains to be investigated.…”

Section: Smc-cm Induce Macrophage Function Through Cxcl12/cxcr4 Signa...mentioning

confidence: 99%

Myometrial‐derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice

Zhang

Mamillapalli

Habata

et al. 2022

J Cellular Molecular Medi

View full text Add to dashboard Cite

Preterm birth is a major contributor to neonatal mortality and morbidity. Infection results in elevation of inflammation‐related cytokines followed by infiltration of immune cells into gestational tissue. CXCL12 levels are elevated in preterm birth indicating it may have a role in preterm labour (PTL); however, the pathophysiological correlations between CXCL12/CXCR4 signalling and premature labour are poorly understood. In this study, PTL was induced using lipopolysaccharide (LPS) in a murine model. LPS induced CXCL12 RNA and protein levels significantly and specifically in myometrium compared with controls (3‐fold and 3.5‐fold respectively). Highest levels were found just before the start of labour. LPS also enhanced the infiltration of neutrophils, macrophages and T cells, and induced macrophage M1 polarization. In vitro studies showed that condition medium from LPS‐treated primary smooth muscle cells (SMC) induced macrophage migration, M1 polarization and upregulated inflammation‐related cytokines such as interleukin (IL)‐1, IL‐6 and tumor necrosis factor alpha (TNF‐α). AMD3100 treatment in pregnant mice led to a significant decrease in the rate of PTL (70%), prolonged pregnancy duration and suppressed macrophage infiltration into gestation tissue by 2.5‐fold. Further, in‐vitro treatment of SMC by AMD3100 suppressed the macrophage migration, decreased polarization and downregulated IL‐1, IL‐6 and TNF‐α expression. LPS treatment in pregnant mice induced PTL by increasing myometrial CXCL12, which recruits immune cells that in turn produce inflammation‐related cytokines. These effects stimulated by LPS were completely reversed by AMD3100 through blocking of CXCL12/CXCR4 signalling. Thus, the CXCL12/CXCR4 axis presents an excellent target for preventing infection and inflammation‐related PTL.

show abstract

“…It was shown that quercetin significantly inhibited the expression levels of RELA, JUN mRNA and protein in lipopolysaccharide-induced mouse sarcolemma and exerted anti-inflammatory and antioxidant effects. [ 41 ] Quercetin regulates c-myc to inhibit apoptosis in cardiomyocytes during cardiac hypertrophy. [ 42 ] Quercetin attenuates sodium nitrite-induced hypoxia in rats by inhibiting the HIF-1α pathway has a protective effect on cardiac tissue.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Epimedium intervention in heart failure based on network pharmacology and molecular docking technology

Boyang

Yiping

et al. 2022

Medicine

View full text Add to dashboard Cite

To analyze the pharmacological mechanism of Epimedium in regulating heart failure (HF) based on the network pharmacology method, and to provide a reference for the clinical application of Epimedium in treating HF. Obtaining the main active ingredients and their targets of Epimedium through TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform) database. Access to major HF targets through Genecards, OMIM, PharmGKB, Therapeutic Target Database, Drug Bank database. Protein interaction analysis using String platform and construction of PPI network. Subsequently, Cytoscape software was used to construct the "Epimedium active ingredient-heart failure target" network. Finally, the molecular docking is verified through the Systems Dock Web Site. The core active ingredients of Epimedium to regulate HF are quercetin, luteolin, kaempferol, etc. The core targets are JUN, MYC, TP53, HIF1A, ESR1, RELA, MAPK1, etc. Molecular docking validation showed better binding activity of the major targets of HF to the core components of Epimedium. The biological pathways that Epimedium regulates HF mainly act on lipid and atherosclerotic pathways, PI3K-Akt signaling pathway, and chemoattractant-receptor activation. And its molecular functions are mainly DNA-binding transcription factor binding, RNA polymerase II-specific DNA-binding transcription factor binding, and neurotransmitter receptor activity. This study reveals the multi-component, multi-target and multi-pathway mechanism of action of Epimedium in regulating mental failure, and provides a basis for the clinical development and utilization of Epimedium to intervene in HF.

show abstract

Quercetin Prevents Lipopolysaccharide-Induced Experimental Preterm Labor in Mice and Increases Offspring Survival Rate

Cited by 8 publications

References 60 publications

Exploring the treatment of COVID‐19 with Yinqiao powder based on network pharmacology

Exploring the treatment of COVID‐19 with Yinqiao powder based on network pharmacology

Myometrial‐derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice

Mechanism of Epimedium intervention in heart failure based on network pharmacology and molecular docking technology

Contact Info

Product

Resources

About