Quercetin Metabolites In Plasma of Rats Fed Diets Containing Rutin or Quercetin

Manach, Claudine; Morand, Christine; Texier, Odile; Favier, Marie-Laure; Agullo, Georgine; Demigné, Christian; Regerat, F; Rémésy, Christian

doi:10.1093/jn/125.7.1911

Cited by 283 publications

(154 citation statements)

References 21 publications

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…There is, to our knowledge, no study assessing the physiologically relevant concentrations that galangin may achieve, but other flavonoids have been measured in the plasma and some tissues of humans in the range of concentrations used in this study (Morton et al, 1997;Hollman and Katan, 1998). Moreover, these concentrations correspond to plasma concentrations found in rats fed with a flavonoidenriched diet (Manach et al, 1995). RT-PCR was performed, the product was subjected to electrophoresis, and the gel was analysed and quantified with a phosphoimager.…”

Section: Discussionmentioning

confidence: 96%

The flavonoid galangin is an inhibitor of CYP1A1 activity and an agonist/antagonist of the aryl hydrocarbon receptor

1999

View full text Add to dashboard Cite

SummaryThe effect of the dietary flavonoid galangin on the metabolism of 7,12-dimethylbenz [a]anthracene (DMBA), the activity of cytochrome P 450 1A1 (CYP1A1), and the expression of CYP1A1 in MCF-7 human breast carcinoma cells was investigated. Galangin inhibited the catabolic breakdown of DMBA, as measured by thin-layer chromatography, in a dose-dependent manner. Galangin also inhibited the formation of DMBA-DNA adducts, and prevented DMBA-induced inhibition of cell growth. Galangin caused a potent, dose-dependent inhibition of CYP1A1 activity, as measured by ethoxyresorufin-O-deethylase activity, in intact cells and in microsomes isolated from DMBAtreated cells. Analysis of the inhibition kinetics by double-reciprocal plot demonstrated that galangin inhibited CYP1A1 activity in a noncompetitive manner. Galangin caused an increase in the level of CYP1A1 mRNA, indicating that it may be an agonist of the aryl hydrocarbon receptor, but it inhibited the induction of CYP1A1 mRNA by DMBA or by 2,3,5,7-tetrachlorodibenzo-p-dioxin (TCDD). Galangin also inhibited the DMBA-or TCDD-induced transcription of a reporter vector containing the CYP1A1 promoter. Thus, galangin is a potent inhibitor of DMBA metabolism and an agonist/antagonist of the AhR, and may prove to be an effective chemopreventive agent.

show abstract

Section: Discussionmentioning

confidence: 96%

The flavonoid galangin is an inhibitor of CYP1A1 activity and an agonist/antagonist of the aryl hydrocarbon receptor

1999

View full text Add to dashboard Cite

show abstract

“…To analyze the conjugated metabolites of the flavonoids, other than those of catechin, the HPLC system consisted of an autosampler (Kontron 360), a Diode Array Detector (set at 370 nm for flavonols, 350 nm for flavone, 290 nm for flavanone, and at 280 nm for genistein) and Software system for data recording and processing. The system was fitted with a 5-m C 18 Hypersil BDS analytic column (150 ϫ 4.6 mm; Life Sciences International, Cergy, France). The mobile phases were 15% acetonitrile (solvent A) and 37% acetonitrile (solvent B), each containing 0.5% of H 3PO4.…”

Section: Hplc Analysismentioning

confidence: 99%

“…Circulating forms of absorbed flavonoids have been identified as methylated and/or glucuronidated and/or sulfated metabolites (9,12,19,23,39), resulting from the conjugative activities of both liver and intestine (7). Flavonoid metabolites are then eliminated in urine and by the biliary route (18,22,35,40). When excreted into the bile, flavonoid metabolites flow in the small intestine and reach the hindgut where they can be reabsorbed after their hydrolysis by the microflora (30).…”

mentioning

confidence: 99%

The splanchnic metabolism of flavonoids highly differed according to the nature of the compound

Crespy

Morand

Besson

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

Self Cite

114

101

View full text Add to dashboard Cite

Crespy, Vanessa, Christine Morand, Catherine Besson, Nicole Cotelle, Hervé Vé zin, Christian Demigné , and Christian Ré mé sy. The splanchnic metabolism of flavonoids highly differed according to the nature of the compound. Am J Physiol Gastrointest Liver Physiol 284: G980-G988, 2003; 10.1152/ajpgi.00223.2002.-The absorption and splanchnic metabolism of different flavonoids (namely quercetin, kaempferol, luteolin, eriodictyol, genistein, and catechin) were investigated in rats after an in situ perfusion of jejunum plus ileum (14 nmol/min). Net transfer across the brush border ranged widely according to the perfused compound (from 78% for kaempferol to 35% for catechin). This variation seems linked to the lipophilicity of a given flavonoid rather than to its three-dimensional structure. Except for catechin, conjugated forms of perfused flavonoids were also detected in the intestinal lumen, but the extent of this secretion depended on the nature of the perfused compounds (52% for quercetin to 11% for genistein). For some of the perfused aglycones, biliary secretion was an important excretion route: 30% of the perfused dose for genistein but only 1% for catechin. Thus the splanchnic metabolism of flavonoid is controlled by several factors: 1) the efficiency of their transfer through the brush border, 2) the intensity of the intestinal secretion of conjugates toward the mucosal and serosal sides, respectively, and 3) the biliary secretion of conjugates. These data suggested that the splanchnic metabolism of perfused flavonoids depends on the nature of the compound considered, which in turn influences their availability for peripheral tissues. absorption; biliary excretion; rats FLAVONOIDS are phenolic secondary plant metabolites responsible for much of the color and flavor of plant foods. They are ubiquitous in fruits, vegetables, and beverages, with more than 4,000 chemically different flavonoids identified to date. They are assigned to different groups according on their structure: anthocyanins, flavonols, flavones, catechins, isoflavones, and flavonones (1,8,38,43). Owing to their ubiquity in plants, humans are constantly exposed to a wide variety of flavonoids. A wealth of beneficial properties has been reported: antibacterial, antioxidant, anti-inflammatory, and anticarcinogenic effects (2, 21, 29) and estrogenic effects for isoflavones (33). Understanding the absorption and metabolism of dietary flavonoids is fundamental to determining their potential biological activity.The small intestine is a crucial site for the absorption of dietary flavonoids. In this tissue, aglycones are metabolized by intestinal conjugation enzymes, and the resulting metabolites are then secreted toward the mucosal and/or serosal sides (5, 6, 10, 13, 37). Circulating forms of absorbed flavonoids have been identified as methylated and/or glucuronidated and/or sulfated metabolites (9,12,19,23,39), resulting from the conjugative activities of both liver and intestine (7). Flavonoid metabolites are then eliminated in urine and by the bili...

show abstract

“…Flavonoids can travel in the body while bound to plasma proteins [75,76]. The metabolism of lavonoid glycosides to aglycones and speciic glucuronides occurs in the intestinal tissue, which depends on the structure of the lavonoid [77].…”

Section: Bioavailabilitymentioning

confidence: 99%

Grape Seed Nutraceuticals for Disease Prevention: Current Status and Future Prospects

Ananga¹,

Obuya²,

Ochieng³

et al. 2017

Phenolic Compounds - Biological Activity

View full text Add to dashboard Cite

Grapes (Vitis spp.) are consumed as fresh table fruits, raisins, and processed into wine, juice, jelly and other value-added products. Grapes contain bioactive secondary metabolites (polyphenols), such as proanthocyanins (oliogemeric lavonoids), lavonoids (catechin, epicatechin, and quercetin), and anthocyanins. They have non-lavonoids such as hydroxycinnamic acids (p-coumaric, cinnamic, cafeic, gentisic, ferulic, and vanillic acids), and hydroxybenzoic acids: trihydroxy stilbenes (resveratrol and polydatin). These phytochemicals are of economic importance to pharmaceutical, food and cosmetic industries. Nutraceuticals from grape seeds have potential cardioprotective, anti-cancer, antioxidant, anti-inlammatory, antiviral, neuroprotective, hepatoprotective and antimicrobial properties. Grape seed nutraceuticals have been re-invented in the past few years as a new paradigm in human medicine. In particular, nutraceuticals from grape seeds have been used in stopping wound bleeding, anti-inlammatory agents, pain relief, and anti-diarrhea. In addition, they can be used for the treatment of various human health conditions such as cancer, cholera, smallpox, and nausea as well as eye infections, skin, kidney, liver diseases, etc. Nowadays, consumers are demanding for healthy supplements and personal care products with natural ingredients. Therefore, the present review highlights recent developments and future opportunities of grape seed nutraceuticals for the prevention of human diseases.

show abstract

Quercetin Metabolites In Plasma of Rats Fed Diets Containing Rutin or Quercetin

Cited by 283 publications

References 21 publications

The flavonoid galangin is an inhibitor of CYP1A1 activity and an agonist/antagonist of the aryl hydrocarbon receptor

The flavonoid galangin is an inhibitor of CYP1A1 activity and an agonist/antagonist of the aryl hydrocarbon receptor

The splanchnic metabolism of flavonoids highly differed according to the nature of the compound

Grape Seed Nutraceuticals for Disease Prevention: Current Status and Future Prospects

Contact Info

Product

Resources

About