Quercetin inhibits UV irradiation-induced inflammatory cytokine production in primary human keratinocytes by suppressing NF-κB pathway

Vicentini, Fabiana T. M. C.; Torebjörk, H. E.; Shao, Yinlin; Fonseca, Maria José Vieira; Verri, Waldiceu A.; Fisher, Gary J.; Xu, Yuntao

doi:10.1016/j.jdermsci.2011.01.002

Cited by 154 publications

(130 citation statements)

References 49 publications

(52 reference statements)

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“…Acute exposure to UV irradiation causes sunburn, DNA damage, and connective tissue degradation. Accumulated damage resulting from chronic sun exposure can cause skin cancer and premature skin aging (photoaging) (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

et al. 2013

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Abstract. Pimenta pseudocaryophyllus is a Brazilian native plant that presents high concentrations of flavonoids and other polyphenolic compounds. Herein, we evaluated: (1) the chemical properties of P. pseudocaryophyllus ethanolic extract (PPE), (2) the in vitro antioxidant activity (AA) of PPE and of two different topical formulations (F1 and F2) containing PPE, (3) physico-chemical and functional stability, (4) in vitro release of PPE, and (5) in vivo capacity of formulations to prevent UV-B irradiation-induced skin damage. Results show that the polyphenol and flavonoid contents in PPE were 199.33 and 28.32 mg/g, respectively, and HPLC results show the presence of eugenol, tannic acid, and rutin. Evaluation of the in vitro AA of PPE demonstrated a dose-dependent effect and an IC 50 of 4.75 μg/mL in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3.0 μg/mL in 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The ferric-reducing antioxidant power (FRAP assay) was 0.046 μmol/L trolox equivalent/ μg/mL of extract. Among the AA, only the capacity to scavenge DPPH radical of PPE was maintained in F1 and F2. In addition, both formulations satisfactorily released the extract. The evaluation of the functional stability of F1 and F2 did not demonstrate loss of activity by storage at room temperature and at 4°C/6 months. In irradiated mice, treatment with F1 and F2 added with PPE significantly increased the capacity to scavenge ABTS radical and the FRAP of skin compared to vehicle-treated mice. In conclusion, the present results suggest that formulations containing PPE may be a topical source of antioxidant compounds to decrease oxidative damages of the skin.

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Section: Introductionmentioning

confidence: 99%

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

et al. 2013

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“…It is consensus that there is a relation between oxidative stress and the production of inflammatory mediators including cytokines. In fact, oxidative stress activates the transcription factor NFκB to induce cytokine production (Vicentini et al, 2011). The present data demonstrates that T. sinapou extract inhibited oxidative stress and its antioxidant mechanisms.…”

Section: Discussionmentioning

confidence: 50%

“…At least in part, the pharmacological property of T. sinapou extract to inhibit inflammation and oxidative stress might be related to its flavonoidal content as shown for other plants of Tephrosia genus (Arriaga et al, 2009b;Hegazy et al, 2009;Khalivulla et al, 2008;Reddy et al, 2009) since flavonoids inhibit oxidative stress, inflammation and/or cytokine production, and present NO-dependent effects (Casagrande et al, 2006;Filho et al, 2008;Georgetti et al, 2008;Souto et al, 2011;Valério et al, 2009;Vicentini et al, 2011).…”

Section: Discussionmentioning

confidence: 98%

“…tumor necrosis factor α [TNFα]) activate macrophage NADPH oxidase to produce superoxide anion, which in turn generates hydroxyl radical and peroxynitrite (Bowie & O'Neill, 2000;Valério et al, 2009;Vicentini et al, 2011). Thus, there is a two way route linking infl ammatory mediators and free radicals, and therefore, the interference with this route by antioxidants such as fl avonoids explains their anti-infl ammatory activity, and suggests their application or even of extracts containing these molecules as anti-infl ammatory agents (Andrade et al, 2010;Valério et al, 2009;Vieira et al, 2011;Vicentini et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…flavonoids inhibit inflammation-induced production of free radicals (Casagrande et al, 2006), cytokines (Valério et al, 2009), prostaglandin E (Morikawa et al, 2003), inducible nitric oxide synthase expression and NFκB activation (Vicentini et al, 2011). The modulation of the production of those molecules leads to reduced inflammatory pain (Anjaneyulu & Chopra, 2003;Anjaneyulu & Chopra, 2004;Filho et al, 2008;Valério et al, 2009), edema (Valério et al, 2009), and leukocyte recruitment .…”

Section: Introductionmentioning

confidence: 99%

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Tephrosia sinapou extract reduces inflammatory leukocyte recruitment in mice: effect on oxidative stress, nitric oxide and cytokine production

Martínez

Zarpelon

Zimermann

et al. 2012

Rev. bras. farmacogn.

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Abstract:Tephrosia toxicaria (Sw.) Pers., which is currently known as T. sinapou (Buc'hoz) A. Chev., Fabaceae, is a source of compounds such as fl avonoids, however, few studies addressed the anti-infl ammatory and antioxidant effects of T. sinapou. Therefore, we evaluated the antioxidant mechanisms of the T. sinapou ethyl acetate extract in vitro, and whether the extract affects leukocyte recruitment in four models of infl ammation and the involvement of nitric oxide and cytokines in its mechanism. In vitro, it was observed that the extract presented hydrogen donating ability to 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH • ), 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS + ), and also effi ciently inhibited iron-dependent and independent lipid peroxidation and iron chelation assays. In vivo, it inhibited the recruitment of total leukocytes and neutrophil induced by carrageenin, zymosan, glycogen and lipopolysaccharide in the peritoneal cavity of mice. Two mechanisms were detected: 1) T. sinapou effect on leukocyte recruitment depends on nitric oxide since was dose-dependently inhibited by treatment with L-NAME (nitric oxide synthase inhibitor), and 2) the extract also inhibited the production of crucial cytokines for the leukocyte recruitment; tumor necrosis factor α and interleukin-1β. Concluding, T. sinapou ethyl acetate extract reduces oxidative stress in vitro, and infl ammatory leukocyte recruitment by a mechanism related to inhibition of cytokine production, and in a nitric oxide dependent manner in vivo.

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Antioxidants and Anti‐inflammatories

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2015

Cosmetic Dermatology

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Quercetin inhibits UV irradiation-induced inflammatory cytokine production in primary human keratinocytes by suppressing NF-κB pathway

Cited by 154 publications

References 49 publications

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

Tephrosia sinapou extract reduces inflammatory leukocyte recruitment in mice: effect on oxidative stress, nitric oxide and cytokine production

Antioxidants and Anti‐inflammatories

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