Quercetin inhibits histamine-induced calcium influx in human keratinocyte via histamine H4 receptors

Yang, Chung-Chi; Hung, Yen-Ling; Li, Hsin-Ju; Lin, Ya‐Fan; Wang, Su‐Jane; Chang, Der–Chen; Pu, Chi-Ming; Hung, Chi‐Feng

doi:10.1016/j.intimp.2021.107620

Cited by 20 publications

(21 citation statements)

References 40 publications

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“…Extensive research has been conducted in recent years to elucidate the effects of H1 and H4 receptors on histamine-caused calcium in ux in both in vitro and in vivo settings [9,10] . It has been demonstrated by various researchers that in human epidermal keratinocytes, histamine receptor blockers play an inhibitory role in histamine-induced calcium ion in ux [12,19,27] . To ascertain if the inhibitory effect is associated with histamine receptors, we conducted experiments to evaluate if SL could further inhibit histamine-induced calcium ion in ux when histamine receptor blockers are present.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, the primary keratinocytes is identi ed as the source of TRPV1 [16] . Studies conducted on cultured human HaCaT keratinocytes have demonstrated that the in ux of calcium, mediated by TRPV1, has an inhibitory effect on proliferation and an apoptotic effect [12,17] . TRPV1 is a key component in initiating physiological reactions following the activation of histamine receptors [14] .…”

Section: Introductionmentioning

confidence: 99%

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Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

Ma²,

Chen³

et al. 2023

Preprint

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Biosurfactants are attracting much interest due to their potential application as therapeutic agents in the medical field. Previous studies have demonstrated that biosurfactant such as sophorolipid (SL) exhibits immunomodulatory effects. In this paper, we found the potential of sophorolipid for inhibiting histamine-induced itch and preliminarily explored its molecular basis. First, behavioral tests indicated that SL could remit the histamine-induced scratching behaviors of mice. Second, SL could suppress the the calcium influx induced by histamine, HTMT and VUF8430 in HaCaT cells. RT-PCR analysis showed that the histamine-induced upregulation of mRNA levels of phospholipase Cγ1 (PLCγ1), 1,4,5-trisphosphate receptor (IP3R), and protein kinase Cα (PKCα) could be inhibted by SL, suggesting that SL may impede the PLC-IP3R signaling pathway activated by histamine. In further tests, the capsaicin-induced calcium influx could also be inhibited by SL, and molecular docking analysis indicated the possible binding of SL with transient receptor potential vanilloid-1 (TRPV1). In summary, these results revealed that SL may inhibit histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity. This paper indicates that SL may be a useful treatment medicine for histamine-dependent itch.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

Ma²,

Chen³

et al. 2023

Preprint

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“…When cellular stimulation occurs, Ca 2+ flows into the cell through calcium channels in the cell membrane, resulting in a significant increase in the content of intracellular free Ca 2+ , which is not conducive to the homeostasis of organisms and is easy to conduct stimulation, further exacerbating inflammation. Therefore, both ROS and Ca 2+ play a vital role in maintaining the proliferation and differentiation of cells and in regulating apoptosis [ 20 , 21 ]. These results suggest that SL can reverse the generation of ROS and the influx of Ca 2+ in LPS-induced RAW264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Ma²,

Chen³

et al. 2022

Molecules

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Objectives: Biosurfactants with anti-inflammatory activity may alleviate skin irritation caused by synthetic surfactants in cleaning products. Sophorolipid (SL) is a promising alternative to synthetic surfactants. However, there are few reports on the anti-inflammatory activity of SL and the underlying mechanism. The purpose of this work is to verify that lipopolysaccharide (LPS)-induced inflammation could be inhibited through targeting the pathway of nuclear factor-κB (NF-κB) in RAW264.7 cells. Methods: The influence of SL on cytokine release was investigated by LPS-induced RAW264.7 cells using ELISA. The quantification of the protein expression of corresponding molecular markers was realized by Western blot analysis. Flow cytometry was employed to determine the levels of Ca2+ and reactive oxygen species (ROS). The relative expression of inducible nitric oxide synthase (INOS) and cyclooxygenase-2 (COX-2) was determined by RT-PCR. An immunofluorescence assay and confocal microscope were used to observe the NF-κB/p65 translocation from the cytoplasm into the nucleus. The likely targets of SL were predicted by molecular docking analysis. Results: SL showed anti-inflammatory activity and reduced the release of inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and nitric oxide (NO). The experimental results show that SL suppressed the Ca2+ and ROS levels influx in the LPS-induced RAW264.7 cells and alleviated the LPS-induced expression of iNOS and COX-2, the LPS-induced translocation of NF-κB (p65) from the cytoplasm into the nucleus, and the expression of phosphorylated proteins such as p65 and IκBα. Furthermore, molecular docking analysis showed that SL may inhibit inflammatory signaling by competing with LPS to bind TLR4/MD-2 through hydrophobic interactions and by inhibiting IKKβ activation through the hydrogen bonding and hydrophobic interactions. Conclusion: This study demonstrated that SL exerted anti-inflammatory activity via the pathway of NF-κB in RAW264.7 cells.

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“…Therefore, finding better alternative treatments is indispensable. With ongoing research, it is a promising and feasible method to find new natural chemicals with fewer side effects from medicinal plants to treat AD [ 26 , 27 , 28 ]. In the past, we have researched and analyzed plant components with anti-inflammatory and antioxidant properties.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Anti-Atopic Dermatitis Effects of α-Boswellic Acid on Tnf-α/Ifn-γ-Stimulated HaCat Cells and DNCB-Induced BALB/c Mice

Tsai

Chang

Chou

et al. 2022

IJMS

Self Cite

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Boswellic acids, triterpenoids derived from the genus Boswellia (Burseraceae), are known for their anti-inflammatory and anti-tumor efficacy. Atopic dermatitis is a chronic, non-infectious inflammatory skin disease. However, the effects of α-boswellic acid on atopic dermatitis have not been studied. Therefore, in this study we examined the expression level of pro-inflammatory cytokines, histopathological analysis, and physiological data from BALB/c mice with atopic-like dermatitis induced by 2,4-dinitrochlorobenzene and TNF-α/IFN-γ-stimulated HaCaT cells to better understand the agent’s anti-atopic dermatitis efficacy. First, we found that α-boswellic reduced the epidermal thickening, mast cell numbers, and dermal infiltration of 2,4-dinitrochlorobenzene-induced atopic-like dermatitis in BALB/c mice. Furthermore, we also found that α-boswellic acid can restore transepidermal water loss and skin reddening in mice. In human keratinocytes inflamed by TNF-α/IFN-γ, α-boswellic acid inhibited MAP kinase activation and showed a reduction in NF-κB nuclear translocation. Finally, α-boswellic acid can reduce the expression level of cytokines (IL-1β, IL-6, and IL-8) following the stimulation of TNF-α/IFN-γ in HaCaT cells. Taken together, our study suggests that α-boswellic acids are a potential component for the development of anti-atopic dermatitis drugs.

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Quercetin inhibits histamine-induced calcium influx in human keratinocyte via histamine H4 receptors

Cited by 20 publications

References 40 publications

Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Evaluation of the Anti-Atopic Dermatitis Effects of α-Boswellic Acid on Tnf-α/Ifn-γ-Stimulated HaCat Cells and DNCB-Induced BALB/c Mice

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