Quercetin Inhibits Chronic Stress-Induced Myocardial Infarction in Rats

Abstract

Ismaeel Bin-Jaliah BIN-JALIAH, I. Quercetin inhibits chronic stress-induced myocardial infarction in rats. Int. J. Morphol., 35(4):1363Morphol., 35(4): -1369Morphol., 35(4): , 2017. SUMMARY:We recently reported that chronic unpredictable stress (CUS) induced left ventricular dysfunction (LVD) in rats, which was inhibited by the cardioprotective agent quercetin. Based on these findings and because majority of patients with myocardial infarction (MI) can develop LVD and heart failure, we sought to produce an animal model of MI and LVD in rats and use this model to test the hypothesis that quercetin is able to prevent the potential MI induction by CUS. Rats were exposed to CUS using a variety of stressors in the presence and absence of quercetin (50 mg/kg body weight/day) for 21 days. Blood pressure and electrocardiogram (ECG) were recorded in all rat groups together with the examination of left ventricle (LV) tissue homogenates and sections to confirm the production of the animal model. We further extend on our recent findings on the role of apoptosis in the pathology of LVD and eventually MI. Blood pressure measurements and ECG recording confirmed the development of systemic hypertension and MI in the model group of rats exposed to CUS. In addition, histological staining confirmed that LV damages occurred in the same group. Furthermore, the proapoptotic gene Bax and the inflammatory biomarkers, TNF-α and IL-6 were augmented in LV homogenates by CUS. Simultaneous quercetin treatment lowered blood pressure and substantially prevented MI since it blocked the elevation of ST segment on the ECG and maintained a normal ECG reading. Quercetin suppressed the expression of Bax RNA messages, and significantly (p<0.05) blocked CUS-induced TNF-α and IL-6 upregulation. Thus, CUS induced MI in rats associated with augmentation of tissue injury biomarkers were prevented by quercetin, which further endorses our recent findings of a potential therapeutic role for quercetin in CUS induced cardiac dysfunction.

Reduction of Doxorubicin-Induced Cardiotoxicity by Co-Administration of Smart Liposomal Doxorubicin and Free Quercetin: In Vitro and In Vivo Studies

“…Doxorubicin was selected as a common chemotherapy drug [ 16 , 17 , 18 , 19 ], and a cumulative dose of 18 mg/kg was used in 6 injections every other day via intraperitoneal injection (IP). Due to the antioxidant role of quercetin [ 20 , 21 , 22 , 23 , 24 ], this herbal substance (purity > 95%) (Sigma, USA) was selected and administered daily by gavage at a dose of 50 mg/kg with a normal saline carrier for 11 days. Rats were randomly divided into seven groups of six members as follows.…”

Quercetin prevents myocardial infarction adverse remodeling in rats by attenuating TGF-β1/Smad3 signaling: Different mechanisms of action

“…Rats were classified into 5 groups (n = 12/group) in the following order: 1) Control sham-operated group: had a similar sham surgical procure without tying the left anterior descending (LAD) coronary artery and then received an equivalent oral dose of 0.5% carboxymethyl cellulose (CMC) (a vehicle), daily, for the next 30 days; 2) Control + QUR treated group: had similar sham surgery as in group but then received QUR (prepared in 0.5% CMC) (50 mg/kg/orally) for 30 days; 3) MI-induced group: were rats with pre-established MI and received 0.5% CMC as a vehicle for the next 30 days starting 1 h after the induction of MI, and 4) MI + QUR-treated group: were rats with pre-established MI and administered QUR solution, 1 h post-induction of MI (50 mg/kg/orally) and continued for the next 30 days starting. The preparation, dose, and route of administration of this QUR were adopted from previous studies, which demonstrated cardioprotective and anti-fibrotic effects of QUR at this dose ( Zaafan et al, 2013 , Bin-Jaliah, 2017 , Wang et al, 2020 ).…”

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

“…Thereby concluded that quercetin has cardio-protective potential and established its mechanism of action against isoproterenol-induced myocardial infarction (MI) in experimental rats. The inhibition of chronic-unpredictable-stress-(CUS)-induced left ventricular dysfunction (LVD) in rats by quercetin in an animal model was studied by Bin-Jaliah [40]. It was hypothesized that quercetin tends to prevent myocardial infarction (MI) induction by CUS.…”

“…Furthermore, the significant inhibition of expression of Bax RNA messages and blocked CUS-induced TNF-α and IL-6 upregulation led to the efficacy of quercetin treatment. Thus, a possible therapeutic role for quercetin in CUS-induced cardiac dysfunction was established by Bin-Jaliah [40]. Quercetin was studied for its effect on myocardial ischemia in patients with stable coronary heart disease (CHD) by Chekalina et al [41].…”