Quercetin induces cell-cycle G1 arrest through elevating Cdk
inhibitors p21 and p27 in human hepatoma cell line (HepG2)

Mu, Cuicui; Jia, Pei-Min; Yan, Zhong; Liu, X; Li, X; Liu, H

doi:10.1358/mf.2007.29.3.1092095

Cited by 86 publications

(49 citation statements)

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“…This is further supported by decreased levels of CDK2, CDK6, cyclin D, cyclin E, cyclin A, and pRb accompanied by induction of p21 and p27. Accordingly, Mu and colleagues (35) demonstrated that in HepG2 human hepatoma cells, quercetin blocks cell-cycle progression at G 1 phase and exerts this effect through the p21, p27, and p53 increase. p21 is an important growth arrest mediator and a CDK activity regulator, inhibiting cell entry into G 1 phase in response to DNA damage and blocking the reentry of G 2 cells into S phase by blocking cyclin E-CDK2-mediated Rb phosphorylation (36).…”

Section: Discussionmentioning

confidence: 99%

Multitarget Effects of Quercetin in Leukemia

Maso

Calgarotto

Franchi

et al. 2014

Cancer Prevention Research

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This study proposes to investigate quercetin antitumor efficacy in vitro and in vivo, using the P39 cell line as a model. The experimental design comprised leukemic cells or xenografts of P39 cells, treated in vitro or in vivo, respectively, with quercetin; apoptosis, cell-cycle and autophagy activation were then evaluated. Quercetin caused pronounced apoptosis in P39 leukemia cells, followed by Bcl-2, Bcl-xL, Mcl-1 downregulation, Bax upregulation, and mitochondrial translocation, triggering cytochrome c release and caspases activation. Quercetin also induced the expression of FasL protein. Furthermore, our results demonstrated an antioxidant activity of quercetin. Quercetin treatment resulted in an increased cell arrest in G 1 phase of the cell cycle, with pronounced decrease in CDK2, CDK6, cyclin D, cyclin E, and cyclin A proteins, decreased Rb phosphorylation and increased p21 and p27 expression. Quercetin induced autophagosome formation in the P39 cell line. Autophagy inhibition induced by quercetin with chloroquine triggered apoptosis but did not alter quercetin modulation in the G 1 phase. P39 cell treatment with a combination of quercetin and selective inhibitors of ERK1/2 and/or JNK (PD184352 or SP600125, respectively), significantly decreased cells in G 1 phase, this treatment, however, did not change the apoptotic cell number. Furthermore, in vivo administration of quercetin significantly reduced tumor volume in P39 xenografts and confirmed in vitro results regarding apoptosis, autophagy, and cell-cycle arrest. The antitumor activity of quercetin both in vitro and in vivo revealed in this study, point to quercetin as an attractive antitumor agent for hematologic malignancies. Cancer Prev Res; 7(12); 1240-50. Ó2014 AACR.

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Section: Discussionmentioning

confidence: 99%

Multitarget Effects of Quercetin in Leukemia

Maso

Calgarotto

Franchi

et al. 2014

Cancer Prevention Research

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“…Recent studies have demonstrated that flavonoids modify various cellular functions, such as proliferation [7], apoptosis [8], and transcription and/or translation [9], in manners dependent on or independent of its antioxidant properties. In addition, it has been reported that some flavonoids and flavonoid derivatives affect ion transport [10-14, 19, 20].…”

Section: Discussionmentioning

confidence: 99%

“…Recent studied have revealed that flavonoids modulate various cellular functions, such as cell cycle progression [7], apoptosis [8], and gene expression [9]. In addition, our previous studies have indicated that some flavonoids stimulate ion transport [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Quercetin Stimulates NGF-induced Neurite Outgrowth in PC12 Cells via Activation of Na+/K+/2Cl- Cotransporter

Nakajima

Niisato

Marunaka

2011

Cell Physiol Biochem

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We have recently reported that Na⁺/K⁺/2Cl^- cotransporter isoform 1 (NKCC1) plays an essential role in nerve growth factor (NGF)-induced neurite outgrowth in PC12D cells. On the other hand, it has been reported that dietary flavonoids, such as quercetin, apigenin, and luteolin, stimulate various ion transporters. In the present report, we investigated the effect of quercetin, a flavonoid, on NGF-induced neurite outgrowth in PC12 cells (the parental strain of PC12D cells). Quercetin stimulated the NGF-induced neurite outgrowth in a dose-dependent manner. Knockdown of NKCC1 by RNAi methods abolished the stimulatory effect of flavonoid. Quercetin stimulated NKCC1 activity (measured as bumetanide-sensitive ⁸⁶Rb influx) without any increase in the expression level of NKCC1 protein. The stimulatory effect of quercetin on neurite outgrowth was dependent upon extracellular Cl^-. These observations indicate that quercetin stimulates the NGF-induced neurite outgrowth via an increase in Cl^- incorporation into the intracellular space by activating NKCC1 in PC12 cell.

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“…Rutin may be useful for the prevention and treatment of colorectal carcinogenesis [13]. Quercetin could be a constituent of chemotherapeutic drugs for the treatment of prostate and skin cancer, because it shows a growthinhibitory effect to tumorigenic cells [14,15]. Although some in vivo studies reported genotoxic and carcinogenic effect of quercetin in rats [16,17], quercetin is not classifiable as carcinogenic to humans [18].…”

Section: Introductionmentioning

confidence: 99%

Rutin and Total Quercetin Content in Amaranth (Amaranthus spp.)

Kalinová

Dadáková

2008

Plant Foods Hum Nutr

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The aim of the study was to confirm the presence of rutin, one of the most common quercetin glycosides, and other quercetin derivatives in plants of genus Amaranthus, to investigate the influence of the species and variety on rutin distribution in the plant and content changes during growing season. The rutin content was determined by micellar electrokinetic capillary chromatography in individual plant parts at the beginning of the growth, at the flowering stage and at the maturity stage of five Amaranthus species. The total quercetin content was determined by micellar electrokinetic capillary chromatography too. The rutin content in amaranth ranged from 0.08 (in seeds) to 24.5 g/kg dry matter (in leaves). Comparison of the determined total quercetin content and the calculated content of quercetin released from rutin did not prove important presence of quercetin or other quercetin derivatives than rutin. Only amaranth leaves sampled at the maturity stage probably contained quercetin or quercetin derivatives. Significant differences in the rutin content were established among species and as well varieties. Amaranthus hybrid and A. cruentus were the best sources of rutin.

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Quercetin induces cell-cycle G1 arrest through elevating Cdk inhibitors p21 and p27 in human hepatoma cell line (HepG2)

Cited by 86 publications

References 0 publications

Multitarget Effects of Quercetin in Leukemia

Multitarget Effects of Quercetin in Leukemia

Quercetin Stimulates NGF-induced Neurite Outgrowth in PC12 Cells via Activation of Na<sup>+</sup>/K<sup>+</sup>/2Cl<sup>-</sup> Cotransporter

Rutin and Total Quercetin Content in Amaranth (Amaranthus spp.)

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