Quercetin-gold nanorods incorporated into nanofibers: development, optimization and cytotoxicity

Mahmoud, Nouf N.; Qabooq, Haneen; Alsotari, Shrouq; Tarawneh, Ola; Aboalhaija, Nour; Shraim, Sawsan; Alkilany, Alaaldin M.; Khalil, Enam A.; Abu-Dahab, Rana

doi:10.1039/d1ra02004h

Cited by 9 publications

(7 citation statements)

References 71 publications

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“…We demonstrated in our previous work the cytotoxicity of GNR decorated with a phospholipid moiety (GNR (A)) towards a panel of breast cancer cell lines and their modulation effect on several regulatory factors involved in cellular apoptosis and energy metabolism [24][25][26]. Furthermore, GNR-decorated with a cholesterol moiety (GNR (B)) were utilized as an efficient nanocarrier for several drugs with relatively low cytotoxicity towards normal healthy cells [27,28,37]. In this study, we used the chicken embryo and its CAM to provide insight into the impact of these two GNR preparations on the early stage of embryogenesis and their possible anti-angiogenic activity to support their previously observed anti-cancer outcome, as normal development shares several major events with carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Surface-Modified Gold Nanorods on the Early Stage of Embryonic Development and Angiogenesis: Insight into the Molecular Pathways

Mahmoud

Zakaria

Kheraldine

et al. 2021

IJMS

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Gold nanorods have been implicated in several biomedical applications. Herein, the effect of two surface-modified gold nanorods on the early stages of embryogenesis and angiogenesis was investigated using avian embryos at three days and their chorioallantoic membrane (CAM) at five days of incubation. We found that gold nanorods (GNR) modified with PEGylated phospholipid moiety show a high mortality rate in embryos after four days of exposure compared to GNR modified with PEGylated cholesterol moiety. Meanwhile, our data revealed that surface modified-GNR significantly inhibit the formation of new blood vessels in the treated CAM model after 48 h of exposure. Moreover, we report that surface-modified GNR significantly deregulate the expression of several genes implicated in cell proliferation, invasion, apoptosis, cellular energy metabolism, and angiogenesis. On the other hand, our data point out that GNR treatments can modulate the expression patterns of JNK1/2/3, NF-KB/p38, and MAPK, which could be the main molecular pathways of the nanorods in our experimental models.

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Section: Discussionmentioning

confidence: 99%

“…GNR was synthesized using the seed-mediated method as described previously with slight modifications [37,65]. Cetyltrimethylammonium bromide (CTAB, Sigma Aldrich, St. Louis, MO, USA) and sodium oleate (Sigma Aldrich, USA) were used as surfactants in the synthesis of the GNR.…”

Section: Synthesis Functionalization and Characterization Of Gnrmentioning

confidence: 99%

The Effect of Surface-Modified Gold Nanorods on the Early Stage of Embryonic Development and Angiogenesis: Insight into the Molecular Pathways

Mahmoud

Zakaria

Kheraldine

et al. 2021

IJMS

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“…Gold nanorods (AuNRs) were synthesized using two surfactants as described previously with some modifications [ 41 ]. Briefly, 0.60 mL of ice-cold sodium borohydride (0.010 M, NaBH 4 99%, Sigma Aldrich, Burlington, MA, USA) was added to a mixture of cetyltrimethylammonium bromide (0.20 M, CTAB 99%; Sigma Aldrich, Burlington, MA, USA) and chloroauric acid 99.9% (0.005 M, HAuCl 4 99%, Sigma Aldrich, Burlington, MA, USA) for seed synthesis, which was confirmed by the formation of a light brown-colored solution.…”

Section: Methodsmentioning

confidence: 99%

Biological Performance of Primary Dental Pulp Stem Cells Treated with Gold Nanoparticles

Abuarqoub,

Mahmoud,

Alshaer

et al. 2023

Biomedicines

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Gold nanoparticles (AuNPs) are one of the most stable nanoparticles that have been prevalently used as examples for biological and biomedical applications. Herein, we evaluate the effect of AuNPs on the biological processes of dental pulp stem cells derived from exfoliated deciduous teeth (SHED). Two different shapes of PEGylated AuNPs, rods (AuNR-PEG) and spheres (AuNS-PEG), were prepared and characterized. SHED cells were treated with different concentrations of AuNR-PEG and AuNS-PEG to determine their effect on the stemness profile of stem cells (SCs), proliferation, cytotoxicity, cellular uptake, and reactive oxygen species (ROS), for cells cultured in media containing-fetal bovine serum (FBS) and serum-free media (SFM). Our results showed that both nanoparticle shapes maintained the expression profile of MSC surface markers. Moreover, AuNS-PEG showed a stimulatory effect on the proliferation rate and lower toxicity on SHED, compared to AuNR-PEG. Higher concentrations of 0.5–0.125 nM of AuNR-PEG have been demonstrated to cause more toxicity in cells. Additionally, cells treated with AuNPs and cultured in FBS showed a higher proliferative rate and lower toxicity when compared to the SFM. For cellular uptake, both AuNS-PEG and AuNR-PEG were uptaken by treated cells efficiently. However, cells cultured in SFM media showed a higher percentage of cellular uptake. For ROS, AuNR-PEG showed a significant reduction in ROS at lower concentrations (<0.03 nM), while AuNS-PEG did not show any significant difference compared to the control untreated cells. Thus, our results give evidence about the optimum concentration and shape of AuNPs that can be used for the differentiation of stem cells into specific cell lineages in tissue engineering and regenerative medicine.

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“…As such, in combination with induction actions such as drugs, their extra anti-inflammatory and antibacterial properties should result in a beneficial wound healing impact (139). The average cell viability of human dermal fibroblasts is 76% by electrostatic spinning to develop polymeric nanofiber scaffolds with a 10.3389/fmed.2022.978120 multi-microporous structure, which is much higher than the level of the control group without nanofibers, and could be used effectively for wound healing with reduced cytotoxicity (140). Chitosan has high biocompatibility and promotes cell attachment and proliferation.…”

Section: Tissue Regeneration Scaffoldsmentioning

confidence: 99%

The potential of functionalized dressing releasing flavonoids facilitates scar-free healing

et al. 2022

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Scars are pathological marks left after an injury heals that inflict physical and psychological harm, especially the great threat to development and aesthetics posed by oral and maxillofacial scars. The differential expression of genes such as transforming growth factor-β, local adherent plaque kinase, and yes-related transcriptional regulators at infancy or the oral mucosa is thought to be the reason of scarless regenerative capacity after tissue defects. Currently, tissue engineering products for defect repair frequently overlook the management of postoperative scars, and inhibitors of important genes alone have negative consequences for the organism. Natural flavonoids have hemostatic, anti-inflammatory, antioxidant, and antibacterial properties, which promote wound healing and have anti-scar properties by interfering with the transmission of key signaling pathways involved in scar formation. The combination of flavonoid-rich drug dressings provides a platform for clinical translation of compounds that aid in drug disintegration, prolonged release, and targeted delivery. Therefore, we present a review of the mechanisms and effects of flavonoids in promoting scar-free regeneration and the application of flavonoid-laden dressings.

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Quercetin-gold nanorods incorporated into nanofibers: development, optimization and cytotoxicity

Abstract: Incorporating GNR-Quer into a mixture of 21% PLGA LMWT and 23% poloxamer 407 produced smooth, intact and uniform electrospun nanofibers with enhanced mechanical properties and hydration potential.

Cited by 9 publications

References 71 publications

The Effect of Surface-Modified Gold Nanorods on the Early Stage of Embryonic Development and Angiogenesis: Insight into the Molecular Pathways

The Effect of Surface-Modified Gold Nanorods on the Early Stage of Embryonic Development and Angiogenesis: Insight into the Molecular Pathways

Biological Performance of Primary Dental Pulp Stem Cells Treated with Gold Nanoparticles

The potential of functionalized dressing releasing flavonoids facilitates scar-free healing

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