Quercetin exerts preventive, ameliorative and prophylactic effects on cadmium chloride - induced oxidative stress in the uterus and ovaries of female Wistar rats

Nna, Victor Udo; Usman, Umar; Ofutet, Emmanuel Oleba; Owu, Daniel U.

doi:10.1016/j.fct.2017.02.010

Cited by 89 publications

(62 citation statements)

References 47 publications

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“…Thus, its diminished activity may indicate cellular accumulation of this reactive oxygen species, which are capable of crossing cell membranes [58]. Although subacute (up to 30 days) administration of Cd leads to alterations in enzymatic antioxidant defence in rat uteri and ovaries [29,31,[60][61][62], there is no data concerning subchronic Cd exposure. In the present subchronic study, decreased CAT activity and increased MDA concentration caused by the highest Cd dose seem to confirm disturbed redox balance in the uterus.…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Subchronic Exposure to Cadmium Causes Persistent Changes in the Reproductive System in Female Wistar Rats

Nasiadek

Danilewicz

Klimczak

et al. 2019

Oxidative Medicine and Cellular Longevity

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Cadmium (Cd) is an environmental toxicant and endocrine disruptor in humans and animals, and recent studies have illustrated that the uterus is exceedingly sensitive to Cd toxicity. The aim of the study was to investigate the influence of subchronic (90 days) oral Cd exposure in daily doses of 0.09-4.5 mg/kg b.w. on the balance of sex hormones by estimating estradiol (E2) and progesterone (P) concentrations in the uterus and plasma in comparison with the effects of 17β-E2. Additionally, the uterine weight, histopathological changes in the uterus and ovaries, the regularity of the estrous cycle, Cd bioaccumulation in uterine tissue, and selected biochemical parameters of oxidative stress were determined. A long period of observation (three and six months following the administration period) was used to assess whether the existing effects are reversible. The lowest dose of Cd caused effects similar to 17β-E2: an increase of E2 concentration in the uterus, endometrial epithelium thickness, and disturbed estrous cycle with estrus phase prolongation. The obtained results suggest that Cd causes nonlinear response. Higher doses of Cd caused a significant decrease in E2 concentration in the uterus and plasma, estrous cycle disturbances, endometrium atrophy, and structural damage in the ovaries. This dose additionally induces lipid peroxidation in the uterine tissues. It is noteworthy that a prolonged time of observation after terminating the exposure showed persistent changes in the concentration of E2 in uterine tissue, as well as alterations in estrous cycle phases, and an increase in lipid peroxidation in the uterus. Moreover, significant positive correlations between the plasma E2 concentration and endometrial epithelium thickness in all studied groups were found. In summary, subchronic oral Cd exposure of female rats may result in impaired fertility processes.

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Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Subchronic Exposure to Cadmium Causes Persistent Changes in the Reproductive System in Female Wistar Rats

Nasiadek

Danilewicz

Klimczak

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…The intraperitoneal injection of QE into rats protects them from the testicular injury caused by prior injection of Cd [26]. The administration of QE into experimental animals restores the serum concentrations of reproductive hormones and results in the prevention of apoptosis in follicular cells due to the exposure to Cd [27].…”

Section: Introductionmentioning

confidence: 99%

The In Vitro Anti-Proliferative Interaction of Flavonoid Quercetin and Toxic Metal Cadmium in the 1321N1 Human Astrocytoma Cell Line

2018

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Cadmium (Cd) is a toxic heavy metal occurring in the environment as an industrial pollutant. The systematic accumulation of Cd in the human body may lead to major health problems. Quercetin (QE) is a natural flavonoid widely distributed in plants and is a part of human diet. Many studies have demonstrated the multiple benefits of QE to humans in protecting cells of our bodies. The aim of this study was to investigate the effect of QE and Cd on the proliferation of astrocytoma 1321N1 cells. Results indicated that the simultaneous exposure of the cells to 200 µM QE and 16 μM Cd significantly reduced cell viability to 6.9 ± 1.6% with respect to vehicle-treated cells. Other experiments of QE pre-treatment followed by the exposure to Cd alone or with QE indicated significant but decreased ability of QE or Cd to reduce proliferation of the cells compared to their co-incubation. Our study suggested a synergetic anti-proliferative interaction of Cd and QE in malignantly transformed cells. This adds new information regarding the biological effects of QE.

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“…The ovarian fragments were cultured in Dulbecco's modified eagle medium: nutrient mixture F-12 (DMEM/F12) 1:1 (BioWhittaker TM , Verviers, Belgium) medium supplemented with 10% foetal calf serum (BioWhittaker TM ) and 1% antibiotic-antimycotic solution (Sigma, St. Louis, MO, USA) in 24-well culture plates (Nunc, Inc., International, Roskilde, Denmark, 1 mL/well) both with and without quercetin (AppliChem GmbH, Darmstadt, Germany) at concentrations 0, 1, 10, and 100 ng/mL. These doses are comparable to the quercetin doses used in previous similar in vitro experiments with ovarian cells (Rice et al, 2006;Jia et al, 2011;Nna et al, 2017). Medium alone was taken as control.…”

Section: Isolation and Culture Of Granulosa Cells And Ovarian Folliclesmentioning

confidence: 60%

Quercetin directly promotes rabbit ovarian steroidogenesis

et al. 2019

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<p>The bioflavonoid quercetin is a component of food with numerous biological effects, but its function in reproductive processes remains to be investigated. This study aimed to examine the direct action of quercetin on steroid hormone release in rabbit ovaries. We analysed the effect of quercetin (0, 1, 10, and 100 ng/mL) on cultured rabbit ovarian fragments. The release of progesterone (P4), testosterone (T) and estradiol (E2) were analysed by enzyme immunoassay. Quercetin promoted P4, T, and E2 release by rabbit ovarian fragments. These observations indicate that quercetin can directly stimulate rabbit ovarian steroidogenesis – an essential regulator of reproduction and fecundity. The application of dietary quercetin for control of rabbit reproduction is discussed.</p>

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Quercetin exerts preventive, ameliorative and prophylactic effects on cadmium chloride - induced oxidative stress in the uterus and ovaries of female Wistar rats

Cited by 89 publications

References 47 publications

Subchronic Exposure to Cadmium Causes Persistent Changes in the Reproductive System in Female Wistar Rats

Subchronic Exposure to Cadmium Causes Persistent Changes in the Reproductive System in Female Wistar Rats

The In Vitro Anti-Proliferative Interaction of Flavonoid Quercetin and Toxic Metal Cadmium in the 1321N1 Human Astrocytoma Cell Line

Quercetin directly promotes rabbit ovarian steroidogenesis

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