Quercetin concentration and total flavonoid content of anti-atherosclerotic herbs using aluminum chloride colorimetric assay

Influence of Molecular Structures on Fluorescence of Flavonoids and Their Detection in Mammalian Cells

“…To prepare samples for UV absorption spectroscopy [ 44 ], 5 μL of flavonoid solution (100 mM) prepared in DMSO is added to 5 μL of 0.5% ethanoic solution of DPBA (or 0.1 M aqueous AlCl 3 ) [ 3 , 45 ]. For in vitro imaging, a drop of (1 μL) of this solution is taken on silanized glass slide.…”

“…To stain the cells by DPBA or aluminum chloride, 0.5% DPBA in ethanol or aqueous aluminum chloride solution (0.1 M) was dropped gently on each chip [ 3 , 45 ]. These chips are then incubated at room temperature for 10 min.…”

Fabrication of Dragee Containing Spirulina platensis Microalgae to Enrich Corn Snack and Evaluate Its Sensorial, Physicochemical and Nutritional Properties

“…Methanolic extract was stored in the freezer at −18 • C until the experiments took place. TFC was measured using a small modification of the Tristantini Budi & Amalia method [23]. To determine the adsorption rate of the snack extract, 0.5 mL of the extract was first mixed with 1.5 mL of distilled water and 0.1 mL of potassium acetate of 1 M, and, after 5 min, 0.1 mL of 10% aluminum chloride and 2.8 mL of distilled water were added, and the adsorption rate of the extracts after 30 min at room temperature at 430 nm was read using spectrophotometer (a JenWay 6305 UV-Vis, Bibby Scientific, Staffordshire, UK) and the quercetin concentration of the extracts was determined using a standard curve (R 2 = 0.996).…”

Acceptable Shelf Life of Indonesian Anti-Atherosclerosis Mixed Herbs Based on Bacterial Count and pH Stability

“…Each material in the anti-atherosclerosis herb mixture contained the essential nutrients (Tristantini and Amalia, 2019). For example, curcuma contains polyphenol such as curcuminoid (Rosidi et al, 2016).…”