Quercetin, but Not Its Glycosidated Conjugate Rutin, Inhibits Azoxymethane-Induced Colorectal Carcinogenesis in F344 Rats,

Abstract

The effect of the flavonoid quercetin and its conjugate rutin was investigated on (biomarkers of) colorectal cancer (CRC). Male F344 rats (n = 42/group) were fed 0, 0.1, 1, or 10 g quercetin/kg diet or 40 g rutin/kg diet. Two wk after initial administration of experimental diets, rats were given 2 weekly subcutaneous injections with 15 mg/kg body wt azoxymethane (AOM). At wk 38 post-AOM, quercetin dose dependently (P < 0.05) decreased the tumor incidence, multiplicity, and size, whereas tumor incidences were comparable in control (50%) and rutin (45%) groups. The number of aberrant crypt foci (ACF) in unsectioned colons at wk 8 did not correlate with the tumor incidence at wk 38. Moreover, at wk 8 post-AOM, the number and multiplicity of ACF with or without accumulation of beta-catenin were not affected by the 10 g quercetin/kg diet. In contrast, another class of CRC-biomarkers, beta-catenin accumulated crypts, contained less beta-catenin than in controls (P < 0.05). After enzymatic deconjugation, the plasma concentration of 3'-O-methyl-quercetin and quercetin at wk 8 was inversely correlated with the tumor incidence at wk 38 (r = -0.95, P </= 0.05). Rats supplemented with 40 g rutin/kg diet had only 30% of the (3'-O-methyl-) quercetin concentration of 10 g quercetin/kg diet-fed rats (P < 0.001). In conclusion, quercetin, but not rutin, at a high dose reduced colorectal carcinogenesis in AOM-treated rats, which was not reflected by changes in ACF-parameters. The lack of protection by rutin is probably due to its low bioavailability.

Matrix metalloproteinases and gastrointestinal cancers: Impacts of dietary antioxidants

“…Volate et al [188] found that food supplementation of approximately 3% quercetin exerts significantly beneficial effects by decreasing precancerous lesions through induction of cellular. Dihal et al [189] reported that quercetin inhibits azoxymethane-induced colorectal carcinogenesis in F344 rats. Quercetin was found to be protective Verma S et al .…”

Extracto etanólico de Baccharis genistelloides (carqueja) sobre el cáncer de colon inducido con 1,2-dimetilhidrazina en ratas*

“…Hasta el momento se sabe que las principales acciones farmacológicas del género Baccharis se deben generalmente a la presencia de flavonoides y terpenos; esto conduce al estudio de flavonoides que van a tener actividad antimutagénica (3) . Tanto el cirsiliol, eupatrin, cirsimaritin, genkwanina, rutina y quercetina son algunos de los flavonoides presentes en el género Baccharis (12) , donde principalmente la quercetina sería la que tendría actividad reductora de iones superóxido (ONOO-) y lipoperoxidación (4,12) .…”

“…Es así que flavonoides, como la quercetina presente en el género Baccharis, han mostrado tener efecto antioxidante y anticancerígeno (4) .…”

The influenc of hydrolyzed and non-hydrolyzed linden inflorescence (<i>Tilia cordata</i>) extract on metabolic and transcriptomic profile in rat liver

“…The results of studies shown that rutin is absorbed more slowly than quercetin because it must be hydrolyzed by the cecal microflora, whereas quercetin is absorbed from the small intestine (Crespy et al, 2002;Manach et al, 1997). Dihal et al (2006) revealed that quercetin, but not rutin inhibits azoxymethane-induced colorectal carcinogenesis in F344 rats. The aim of the present study was to investigate if rats which diets were enriched with hydrolyzed and non-hydrolyzed linden extracts have different blood parameters, liver transcriptomic profiles and some parameters of liver tissue antioxidant status.…”