Quercetin Attenuates Brain Oxidative Alterations Induced by Iron Oxide Nanoparticles in Rats

Abstract

Iron oxide nanoparticle (IONP) therapy has diverse health benefits but high doses or prolonged therapy might induce oxidative cellular injuries especially in the brain. Therefore, we conducted the current study to investigate the protective role of quercetin supplementation against the oxidative alterations induced in the brains of rats due to IONPs. Forty adult male albino rats were allocated into equal five groups; the control received a normal basal diet, the IONP group was intraperitoneally injected with IONPs of 50 mg/kg body weight (B.W.) and quercetin-treated groups had IONPs + Q25, IONPs + Q50 and IONPs + Q100 that were orally supplanted with quercetin by doses of 25, 50 and 100 mg quercetin/kg B.W. daily, respectively, administrated with the same dose of IONPs for 30 days. IONPs induced significant increases in malondialdehyde (MDA) and significantly decreased reduced glutathione (GSH) and oxidized glutathione (GSSG). Consequently, IONPs significantly induced severe brain tissue injuries due to the iron deposition leading to oxidative alterations with significant increases in brain creatine phosphokinase (CPK) and acetylcholinesterase (AChE). Furthermore, IONPs induced significant reductions in brain epinephrine, serotonin and melatonin with the downregulation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial transcription factor A (mtTFA) mRNA expressions. IONPs induced apoptosis in the brain monitored by increases in caspase 3 and decreases in B-cell lymphoma 2 (Bcl2) expression levels. Quercetin supplementation notably defeated brain oxidative damages and in a dose-dependent manner. Therefore, quercetin supplementation during IONPs is highly recommended to gain the benefits of IONPs with fewer health hazards.

Assessment of the potential toxic effect of magnetite nanoparticles on the male reproductive system based on immunological and molecular studies

“…These results are also in line with those of El-Shorbagy et al ( 2018) who proved their ability to cross even through blood-placental barriers and accumulate in maternal brain tissue and foetal liver tissue using the same method. Furthermore, the study done by Dora et al (2021) showed an iron deposition in the brain tissue of adult male rats indicating their capability to cross the BBB. The current work also showed the negative effect of MNPs on the normal architecture of testicular tissue in male rats including some morphological abnormalities in the seminiferous and changes, such as vacuolization, detachment, and sloughing, as reported by Sundarraj et al was implicated for the possible harmful effects on reproduction (Reddy et al, 2017).…”

Biological Effect of Quercetin in Repairing Brain Damage and Cerebral Changes in Rats: Molecular Docking and In Vivo Studies

“…Increased levels of MDA that represent an indication of brain damage induced by toxic lipid peroxidation, and the decreased MDA levels in the quercetin group were an indicator for the protective role of quercetin against the toxicity in the brains of rats. Quercetin supplementation in the treated group decreased the MDA levels in the brain tissue [ 47 , 48 ]. The results demonstrated the positive effects of quercetin against brain damage.…”

Cancer metabolism control by natural products: Pyruvate kinase M2 targeting therapeutics

“…Quercetin : Quercetin is a flavonoid present in fruits, such as apples, berries, cherries, and red grapes, as well as vegetables, including onions and broccoli. Quercetin has multiple beneficial effects, including anti‐inflammatory, antioxidant, anticancer, anti‐ischemic, antiviral, and anti‐aging activities (Dora et al, 2021; El‐Far et al, 2020). Jia et al (2018) reported that quercetin treatment downregulated the expression of cell migration marker proteins, such as matrix metalloproteinase‐2 (MMP‐2), matrix metalloproteinase‐9 (MMP‐9), and VEGF in breast cancer (MCF‐7 and MDA‐MB‐231) cells.…”