Quercetin and indole-3-carbinol inhibit extracellular matrix expression in human primary uterine leiomyoma cells

Greco, Stefania; Islam, Soriful; Zannotti, Alessandro; Carpini, Giovanni Delli; Giannubilo, Stefano Raffaele; Ciavattini, Andrea; Ciarmela, Pasquapina

doi:10.1016/j.rbmo.2020.01.006

Cited by 15 publications

(11 citation statements)

References 38 publications

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“…In 2020, Greco et al showed that I3C and quercetin can regulate ECM expression, migration, and proliferation of primary UF cell. Authors found that I3C significantly decreased collagen 1 and fibronectin mRNA expression [143].…”

Section: Indole-3-carbinolmentioning

confidence: 97%

The Evolving Role of Natural Compounds in the Medical Treatment of Uterine Fibroids

Ciebiera

Ali

Prince

et al. 2020

JCM

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Uterine fibroids (UFs) remain a significant health issue for many women, with a disproportionate impact on women of color, likely due to both genetic and environmental factors. The prevalence of UFs is estimated to be approximately 70% depending on population. UF-derived clinical symptoms include pelvic pain, excessive uterine bleeding, gastrointestinal and voiding problems, as well as impaired fertility. Nowadays numerous methods of UF treatment are available—from conservative treatment to invasive surgeries. Selecting an appropriate treatment option should be individualized and adjusted to the patient's expectations as much as possible. So far, the mainstay of treatment is surgery, but their negative impact of future fertility is clear. On the other hand, emerging new pharmaceutical options have significant adverse effects like liver function impairment, hot flashes, bone density loss, endometrial changes, and inability to attempt conception during treatment. Several natural compounds are found to help treat UFs and relieve their symptoms. In this review we summarize all the current available data about natural compounds that may be beneficial for patients with UFs, especially those who want to preserve their future fertility or have treatment while actively pursuing conception. Vitamin D, epigallocatechin gallate, berberine, curcumin, and others are being used as alternative UF treatments. Moreover, we propose the concept of using combined therapies of natural compounds on their own or combined with hormonal agents to manage UFs. There is a strong need for more human clinical trials involving these compounds before promoting widespread usage.

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Section: Indole-3-carbinolmentioning

confidence: 97%

The Evolving Role of Natural Compounds in the Medical Treatment of Uterine Fibroids

Ciebiera

Ali

Prince

et al. 2020

JCM

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“…We assume that the antifibrotic effect of hop on uterine fibroid cells might have been due to the presence of several flavonoids (such as quercetin and kaempferol). In our previous study, we demonstrated that quercetin reduced the expression of fibrotic factors, including collagen and fibronectin, in uterine fibroid cells (20), suggesting the potential antifibrotic role of quercetin against fibroids. In other fibrotic-like conditions, such as keloids, quercetin also induced inhibitory effects on collagen and fibronectin production and the TGF-b/Smad signaling pathway (21,22).…”

Section: Discussionmentioning

confidence: 85%

Hop and artichoke extracts inhibit expression of extracellular matrix components in uterine leiomyoma cells

et al. 2021

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Objective: To screen 14 different plant extracts for their antifibrotic effect on human primary leiomyoma and healthy myometrial cells. Design: Preclinical study. Setting: University research laboratory. Patient(s): Human uterine leiomyoma and matched myometrial tissues were obtained from Caucasian premenopausal women with symptomatic uterine fibroids at the time of hysterectomy. Intervention(s): Primary human leiomyoma and myometrial cells were cultured in the absence or presence of the plant extracts. Main Outcome Measure(s): Quantification of the expression of extracellular matrix components, such as fibronectin 1 (FN1), collagen type I alpha 1 (COL1A1), and versican (VCAN), and the profibrotic growth factor activin A or inhibin beta A subunit (INHBA). Result(s): The cells were treated with the 14 extracts for 48 hours, and we measured FN1 messenger RNA (mRNA) expression. Of the 14 extracts, about (ABO) ABO-2 (hop) and ABO-9 (artichoke) significantly reduced FN1 expression in both the cell types. Next, we evaluated the effect of fractions of these 2 extracts on the mRNA expression of FN1 and other extracellular matrix components, such as COL1A1, VCAN, and INHBA, in leiomyoma and myometrial cells. We found that ABO-2 (hop) and ABO-9 (artichoke) as well as their fractions, ABO-AR-2016-015 (fraction of ABO-2) and ABO-AR-2014-168 (fraction of ABO-9), reduced the mRNA expression of FN1, COL1A1, VCAN, and INHBA in primary leiomyoma cells. In primary myometrial cells, the mRNA expression of FN1, COL1A1, VCAN, and INHBA was not greatly affected. Conclusion(s): These results suggest that the hop and artichoke extracts possess antifibrotic properties and support additional evaluation using in vivo models.

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“…These growth factors, then regulate cell differentiation, remodeling, synthesis, and proliferation of the extracellular matrix. From this review, Anthocyanins, 50 Quercetin, indole-3-carbinol, 100 and joint Curcumae rhizoma-Sparganii rhizoma (CRSR) herb extracts inhibited ECM components (fibronectin, versican, and collagen1A1). 42 Resveratrol also regulated fibronectin, © 2021 the authors.…”

Section: Plants and Phytochemicals Targeting Extracellular Matrix (Ecm) Reduction And Modulating Growth Factorsmentioning

confidence: 99%

“…Pauzi et al of progesterone and estrogen and in CRSR-treated rats and markedly reduced uterine size by altering ECM-associated genes and/or their related pathways.26. Greco et al100 (in vitro) reduced cell migration.…”

mentioning

confidence: 96%

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2021

TJNPR

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Uterine fibroids (leiomyomas) are typical benign smooth muscle pelvic tumors in women of reproductive years. [1][2] Its occurrence depends on age/race, occurs after menarche, 1, 3-4 and has been found to arise in about 4% of women 20-30 years of age, 11%-18% in those that were 30-40years, and 33% in women between 40-60 years. 5 In South-Western Nigeria, a prevalence of 6.83%, 6 and 35%-40% 7 was reported in Akure and Ile-Ife, respectively. Similarly, in the eastern part of Nigeria, a prevalence of 25% in Enugu State, 8 and 41.95% in Calabar were also recorded. 9 Fibroids present as symptomatic or asymptomatic forms, with a larger percentage of fibroid patients being asymptomatic and having multiple/solitary neoplastic growths. Symptomatic fibroids, on the other hand, accounts for about 25-50% of presented cases, 1-2, 10 characterized by reproductive complications, pelvic pressure (bladder and bowel), abnormal uterine bleeding/acute pelvic pain, hemorrhage or infection, abdominal protrusion, frequent urination and bloating as symptoms. 2,[11][12] Leiomyomas stem from the excessive growth of connective and smooth muscle uterine tissues due to an alteration of normal myocytes, as well as high levels of genomic instability in

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Quercetin and indole-3-carbinol inhibit extracellular matrix expression in human primary uterine leiomyoma cells

Cited by 15 publications

References 38 publications

The Evolving Role of Natural Compounds in the Medical Treatment of Uterine Fibroids

The Evolving Role of Natural Compounds in the Medical Treatment of Uterine Fibroids

Hop and artichoke extracts inhibit expression of extracellular matrix components in uterine leiomyoma cells

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