Quercetin ameliorates peripheral nerve ischemia–reperfusion injury through the NF-kappa B pathway

Abstract

This study aimed to investigate the protective effect of quercetin against ischemia-reperfusion (IR) injury induced in the sciatic nerve of the rat. Quercetin (20 mg/kg) was given during ischemia just before reperfusion. Four groups of rats (Q+IR3, Q+IR7, Q+IR14, and Q+IR28) received 3, 7, 14, and 28 days of reperfusion, respectively, after the intraperitoneal injection of quercetin. After reperfusion, a behavioral test was performed and the sciatic functional index was calculated. Each sciatic nerve was stained to check for edema and ischemic fiber degeneration. Immunohistochemical staining was performed to detect TNF-alpha and NF-kappa B, and TUNEL staining was carried out to detect apoptosis. The Q+IR3, Q+IR7, and Q+IR14 groups showed significantly increased behavioral scores and ameliorated sciatic functional index values compared to IR-injured rats that received vehicle alone during ischemia and then the same period of reperfusion. The Q+IR3, Q+IR7, Q+IR14, and Q+IR28 groups presented significant ischemic fiber degeneration (IFD), TNF-alpha expression, and apoptosis as compared with the IR-injured and perfused rats that did not receive quercetin. The Q+IR3, Q+IR7, and Q+IR28 groups also exhibited significantly decreased NF-kappa B expression (p < 0.001, p = 0.001, p = 0.026) as compared with the IR-injured rats that were perfused but did not receive quercetin. These results imply that quercetin may be beneficial in the treatment of sciatic IR injury because of its antiapoptotic and antiinflammatory effects and its ability to decrease the expression of NF-kappa B.

A morphological and biochemical evaluation of the effects of quercetin on experimental sciatic nerve damage in rats

“…Bcl-2-associated × protein expression, caspase-3 and caspase-9 activation and levels of p53 mRNA, a protein that regulates the cell cycle, increased significantly in a dose-dependent manner in neuroblastoma cells taken from mice administered with quercetin ( 58 ). Gholami et al ( 59 ) reported that quercetin may be beneficial in the treatment of sciatic ischemic/reperfusion injury due to its anti-apoptotic and anti-inflammatory effects and its ability to reduce the expression of nuclear factor-κB. In the present study, the AI in Schwann cells following sciatic nerve damage was significantly increased the T-7 and T-28 groups compared with the S-7 and S-28 groups, respectively.…”

Quercetin restores corticosteroid sensitivity in cells from patients with chronic obstructive pulmonary disease

“…Several limitations are acknowledged in our study. First, quercetin has a wide variety of sites of action including PI3K/Akt, [ 40 ] NF-κB [ 41 ] and estrogen receptor, [ 42 ] and it is difficult to exclude the involvement of such other mechanisms. For example, because activated PI3K/Akt/mTOR pathways are known to induce corticosteroid insensitivity, [ 9,10 ] quercetin could restore corticosteroid insensitivity also via that pathway.…”

Analgesic Effect of Dizocine Combined with Ropivacaine on Recurrent Neuropathic Pain in Peripheral Nerve Compression Rats

“…Peripheral nerve damage leads to microvascular damage, which can lead to the formation of free oxygen free radicals and lipid peroxidation [23]. The formation of free radicals can accelerate tissue damage and induce oxidative stress [24]. In the present study, Schwann cells underwent pronounced phagocytic transformation, which is an indicator of the natural healing process.…”