Quercetin ameliorates liver injury induced with Tripterygium glycosides by reducing oxidative stress and inflammation

Abstract

Quercetin (Que) is one of main compounds in Lysimachia christinae Hance (Christina loosestrife), and has both medicinal and nutritional value. Glycosides from Tripterygium wilfordii Hook.f. (léi gōng téng [the thunder duke vine]; TG) have diverse and broad bioactivities but with a high incidence of liver injury. Our previous study reported on the hepatoprotective properties of an ethanol extract from L. christinae against TG-induced liver injury in mice. This research is designed to observe, for the first time, the possible protective properties of the compound Que against TG-induced liver injury, and the underlying mechanisms that are involved in oxidative stress and anti-inflammation. The results indicated that TG caused excessive elevation in serum levels of alanine/aspartate transaminase (ALT/AST), alkaline phosphatase (ALP), gamma glutamyl transferase (γ-GT), and pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α), as well as hepatic lipid peroxidation (all P < 0.01). On the other hand, following TG exposure, we observed significantly reduced levels of biomarkers, including hepatic glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and the anti-inflammatory cytokine interleukin (IL)-10, as well as the enzyme activity and mRNA expression of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) (all P < 0.01). Nevertheless, all of these alterations were reversed by the pre-administration of Que or the drug bifendate (positive control) for 7 consecutive days. Therefore, this study suggests that Que ameliorates TG-induced acute liver injury, probably through its ability to reduce oxidative stress and its anti-inflammatory properties.

Exocrine Function of the Liver in Rats With Exposure to Cоrvitin

“…Результати досліджень, проведених in vitro, свідчать про різні його біологічні ефекти, зокрема протизапальний, антиоксидантний та судинорозширювальний [2]. Завдяки таким властивостям кверцетин успішно застосовується при хворобах печін-ки, покращуючи біомаркери тканини цього органу при ураженнях різного характеру [3,4], а його екстракти, одержані з лікарських рослин, мають виражену гепатопротекторну й жовчогінну дію та впливають на склад жовчі [5,6]. Як і більшість флавоноїдів, кверцетин має низьку біодоступність для організму, що обмежує вивчення особливостей його ефектів in vivo.…”

Isatidis Folium alleviates acetaminophen‐induced liver injury in mice by enhancing the endogenous antioxidant system

“…33,35 GPx, SOD, and CAT are important enzymes that could promote the conversion of GSSG to GSH. 36 SOD is one of the main enzymes that could convert superoxide radical to hydrogen peroxide, 37,38 and GPx and CAT are both key enzymes that believed to have the principal roles in detoxifying hydrogen peroxide to water and oxygen. [39][40][41] Hydrogen peroxide and hydrogen peroxide are known oxygen free radicals that oxidize GSH to GSSG, 38,42,43 thus increasing the enzymatic activities of GST, GPx, SOD, and CAT could benefit the elevation of hepatic GSH.…”

“…36 SOD is one of the main enzymes that could convert superoxide radical to hydrogen peroxide, 37,38 and GPx and CAT are both key enzymes that believed to have the principal roles in detoxifying hydrogen peroxide to water and oxygen. [39][40][41] Hydrogen peroxide and hydrogen peroxide are known oxygen free radicals that oxidize GSH to GSSG, 38,42,43 thus increasing the enzymatic activities of GST, GPx, SOD, and CAT could benefit the elevation of hepatic GSH. In the current study, IF significantly restored APAP-induced reduction of the GSH in mice liver, which further confirmed that hepatic GSH-related enzymes were involved in the protective actions of IF against APAP-induced hepatic oxidative injury (shown in Figure 5).…”

Effects and mechanism of Tripterygium wilfordii on chronic glomerulo nephritis

“…Tripterygium wilfordii is a plant of the family Celastraceae. It has been shown that Tripterygium wilfordii has pharmacological properties such as anti-inflammatory, immunity regulation, anti-tumor, and reproduction suppression effects, so it is widely applied for treating rheumatoid arthritis, glomerular nephritis, lupus erythematosus, and many autoimmune and skin diseases with significant effects (Wang et al, 2015;Su et al, 2015). In this study, the clinical effects of T. wilfordii on CGN were observed, aiming to further investigate its mechanism of action.…”