Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway

Tseng, Hsiao-Ling; Li, Chia-Jung; Huang, Lin-Huang; Chen, Chun‐Yao; Tsai, Chun‐Hao; Lin, Chun‐Nan; Hsu, Hsue-Yin

doi:10.1016/j.taap.2012.07.022

Cited by 47 publications

(25 citation statements)

References 57 publications

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“…40) Quercetin, kaempferol, and myricetin, which act as ROS scavengers, suppressed the production of ROS in cancer cells and lowered PI3K/Akt and MAPK/Erk signals. 53) These changes are thought to result in the prevention of growth and metastasis of colon and prostate cancer cells. 53) Other studies also demonstrated that antioxidant activities reduced MMP expression and tumor invasion.…”

Section: Discussionmentioning

confidence: 99%

“…53) These changes are thought to result in the prevention of growth and metastasis of colon and prostate cancer cells. 53) Other studies also demonstrated that antioxidant activities reduced MMP expression and tumor invasion. [54][55][56] Based on these reports, it is likely that antioxidants in UE could greatly suppress PI3K/Akt signaling and prevent prostate cancer cell metastasis.…”

Section: Discussionmentioning

confidence: 99%

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Unripe Rubus coreanus Miquel suppresses migration and invasion of human prostate cancer cells by reducing matrix metalloproteinase expression

Kim

Lee

Kim

2014

Bioscience, Biotechnology, and Biochemistry

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Rubus coreanus Miquel (RCM) is used to promote prostate health and has been shown to have anti-oxidant and anti-carcinogenic activities. However, the effects and mechanisms of RCM on prostate cancer metastasis remain unclear. PC-3 and DU 145 cells were treated with ethanol or water extract of unripe or ripe RCM and examined for cell invasion, migration, and matrix metalloproteinases (MMPs) activity and expression. Phosphoinositide 3-kinase (PI3K) and Akt activities were examined. Unripe RCM extracts exerted significant inhibitory effects on cell migration, invasion, and MMPs activities. A significant reduction in MMPs activities by unripe RCM ethanol extract treatment (UE) was associated with reduction of MMPs expression and induction of tissue inhibitors of metalloproteinases (TIMPs) expression. Furthermore, PI3K/Akt activity was diminished by UE treatment. In this study, we demonstrated that UE decreased metastatic potential of prostate cancer cells by reducing MMPs expression through the suppression of PI3K/Akt phosphorylation, thereby decreasing MMP activity and enhancing TIMPs expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unripe Rubus coreanus Miquel suppresses migration and invasion of human prostate cancer cells by reducing matrix metalloproteinase expression

Kim

Lee

Kim

2014

Bioscience, Biotechnology, and Biochemistry

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“…2,7-Dichlorodihydrofluorescein diacetate (H 2 DCFDA; Molecular Probes, Eugene, OR) can be deacetylated inside the cells and then reacted quantitatively with intracellular radicals resulting in its conversion to 2,7-dichlorofluorescent (DCF) byproduct retained within the cells (Tseng et al, 2012). Production of ROS was analyzed by flow cytometry as described previously (Hour et al, 2000).…”

Section: Quantitative Analysis Of Intracellular Rosmentioning

confidence: 99%

Anti-cancer effects of ursane triterpenoid as a single agent and in combination with cisplatin in bladder cancer

Lin

Huang

Lin

et al. 2014

European Journal of Pharmacology

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“…In addition, quercetin has been shown to block JNK- and p38 MAPK-related signaling triggered by oxidation, and it may regulate the expression of apoptotic downstream genes, thereby preventing apoptosis and promoting cell survival [23]. Quercetin 3-O-methyl ether has been shown to protect FL83B liver cells from Cu (2+)-induced apoptosis and mitochondrial dysfunction, and PI3K, Akt and Erk have been shown to be critically involved in the survival of such quercetin ether-treated cells [24]. We hypothesize that the regulation of PI3K and Bax by the F1 fraction may be due to its quercetin derivatives.…”

Section: Discussionmentioning

confidence: 99%

Active subfractions of Abelmoschus esculentus substantially prevent free fatty acid-induced β cell apoptosis via inhibiting dipeptidyl peptidase-4

et al. 2017

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Lipotoxicity plays an important role in exacerbating type 2 diabetes mellitus (T2DM) and leads to apoptosis of β cells. Recently dipeptidyl peptidase-4 (DPP-4) inhibitors have emerged as a useful tool in the treatment of T2DM. DPP-4 degrades type 1 glucagon-like peptide (GLP-1), and GLP-1 receptor (GLP-1R) signaling has been shown to protect β cells by modulating AMPK/mTOR, PI3K, and Bax. The anti-hyperglycemic effect of Abelmoschus esculentus (AE) is well known, however its mucilage makes it difficult to further examine this effect. In our recent report, a sequence of extraction steps was used to obtain a series of subfractions from AE, each with its own composition and property. Among them F1 (rich in quercetin glucosides and pentacyclic triterpene ester) and F2 (containing large amounts of carbohydrates and polysaccharides) were found to be especially effective in attenuating DPP-4 signaling, and to have the potential to counter diabetic nephropathy. Hence, the aim of the present study was to investigate whether AE subfractions can prevent the palmitate-induced apoptosis of β cells, and the putative signals involved. We demonstrated that AE, and especially 1 μg/mL of F2, decreased palmitate-induced apoptosis analyzed by flow cytometry. The result of western blot revealed that palmitate-induced decrease in GLP-1R and increase in DPP-4 were restored by F1 and F2. The DPP-4 inhibitor linagliptin decreased the expression of caspase 3, suggesting that DPP-4 is critically involved in apoptotic signaling. Analysis of enzyme activity revealed that palmitate increased the activity of DPP4 nearly 2 folds, while F2 especially inhibited the activation. In addition, AMPK/mTOR, PI3K and mitochondrial pathways were regulated by AE, and this attenuated the palmitate-induced signaling cascades. In conclusion, AE is useful to prevent the exacerbation of β cell apoptosis, and it could potentially be used as adjuvant or nutraceutical therapy for diabetes.

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Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway

Cited by 47 publications

References 57 publications

Unripe Rubus coreanus Miquel suppresses migration and invasion of human prostate cancer cells by reducing matrix metalloproteinase expression

Unripe Rubus coreanus Miquel suppresses migration and invasion of human prostate cancer cells by reducing matrix metalloproteinase expression

Anti-cancer effects of ursane triterpenoid as a single agent and in combination with cisplatin in bladder cancer

Active subfractions of Abelmoschus esculentus substantially prevent free fatty acid-induced β cell apoptosis via inhibiting dipeptidyl peptidase-4

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