Quenching of Pyrene Derivatives' Fluorescence by Nitroxide Radicals in Sodium Dodecyl Sulfate Micellar Solutions

Angelescu, Daniel G.; Vasilescu, Marilena

doi:10.1006/jcis.2001.7900

Cited by 14 publications

(9 citation statements)

References 47 publications

(47 reference statements)

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“…Here we obtained indirect confirmation of a second quenching in living cells. The hypothesis stems from the noteworthy increase of Even if the nature of the second quencher is not yet completely clarified, ROS are good candidates, as it is already known to quench PBA lifetime [11,12]. We investigated this hypothesis with chemotherapy antracycline drug.…”

Section: Pba Fluorescence Quenchingmentioning

confidence: 97%

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Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Rharass

Ribou²,

Vigo³

et al. 2005

Free Radical Research

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We investigated the fluorescence lifetime of pyrene butyric acid (PBA) using various O2 concentrations in cells. Both in living and freshly fixed cells, PBA lifetime decreased with oxygen concentration. We recorded decay curves in single cells and measured PBA lifetime and NAD(P)H intensity values. Under nitrogen atmosphere, the probe lifetime differences (199 and 209 ns in living and freshly fixed cells, respectively) suggest a supplemental pathway for the deactivation of the probe when the cell functions are not stopped. We propose reactive oxygen species (ROS) to be the additional quenchers that cause this decrease. We further studied the effect of drugs generating ROS the anthracycline doxorubicin (adriamycin). For living cells, PBA lifetime decreased after adriamycin (ADR) treatment (200 and 1000 ng/ml). This supports our hypothesis that under nitrogen atmosphere and for freshly fixed cells, PBA lifetimes increase to an unchanging value due to absence of quenchers.

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Section: Pba Fluorescence Quenchingmentioning

confidence: 97%

“…We suggest that free radicals could affect the lifetime of PBA in living cells. Indeed, it was demonstrated earlier that free radicals can act as fluorescence quenchers of probes such as pyrene derivatives [11,12] in solution. In living cells, the mitochondrial electron transport chain accounts for most of the oxygen consumed in a cell.…”

Section: Introductionmentioning

confidence: 99%

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Rharass

Ribou²,

Vigo³

et al. 2005

Free Radical Research

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“…Therefore, selecting a suitable probe is very necessary. Pyrene has been proven to be a valuable probe molecule and has been successfully used for investigation of dring silica gel [17], to determine aggregation numbers, critical micelle concentration (cmc), microviscosity, and micropolarity of the micellar interior [18,19]. The absorbance spectra of pyrene probe is used to monitor the phase transition or microstructural changes of the microemulsion at different compositions.…”

Section: Uv-absorbance Of Pyrene Probementioning

confidence: 99%

Effect of the spacer group of cationic gemini surfactant on microemulsion phase behavior

Chen

Shang

Liu

et al. 2006

Journal of Colloid and Interface Science

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“…It was then put forward that a supplemental pathway for the deactivation of PBA in living cells is needed and that the influence of free radicals could affect the lifetime of PBA. Indeed, it was demonstrated earlier that free radicals can act as fluorescence quenchers of probes like pyrene derivatives (18,19).…”

Section: Introductionmentioning

confidence: 99%

Lifetime of Fluorescent Pyrene Butyric Acid Probe in Single Living Cells for Measurement of Oxygen Fluctuation^¶

Ribou

Vigo

Salmon

2004

Photochem & Photobiology

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We study the fluorescence lifetime of the well‐known 1‐pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern‐Volmer plot of these solutions is linear, and the bimolecular reaction rate constant agrees with previous observations. In single living cells, the PBA lifetime decreases with oxygen concentration (185 to 55 ns). The probe lifetime differences between living cells and liposome suspension, especially under nitrogen atmosphere, suggest a supplemental pathway for the deactivation of the probe. We simplify further the complex living cells system by stopping the cell functions and studying freshly fixed cells. In this case, we obtained an increase of PBA lifetime under nitrogen atmosphere (215 ns).

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Quenching of Pyrene Derivatives' Fluorescence by Nitroxide Radicals in Sodium Dodecyl Sulfate Micellar Solutions

Cited by 14 publications

References 47 publications

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Effect of the spacer group of cationic gemini surfactant on microemulsion phase behavior

Lifetime of Fluorescent Pyrene Butyric Acid Probe in Single Living Cells for Measurement of Oxygen Fluctuation^¶

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Quenching of Pyrene Derivatives' Fluorescence by Nitroxide Radicals in Sodium Dodecyl Sulfate Micellar Solutions

Cited by 14 publications

References 47 publications

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Effect of the spacer group of cationic gemini surfactant on microemulsion phase behavior

Lifetime of Fluorescent Pyrene Butyric Acid Probe in Single Living Cells for Measurement of Oxygen Fluctuation¶

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Lifetime of Fluorescent Pyrene Butyric Acid Probe in Single Living Cells for Measurement of Oxygen Fluctuation^¶