Quenching of CdSe quantum dot emission, a new approach for biosensing

Dyadyusha, Larysa; Yin, Huabing; Jaiswal, Supriya L.; Brown, Tom; Baumberg, J.J.; Booy, F.; Melvin, T.

doi:10.1039/b500664c

Cited by 206 publications

(134 citation statements)

References 30 publications

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“…Melvin et al have developed a fluorescence competition assay for DNA detection using QDs and gold nanoparticles as a FRET donor-acceptor couple. [78] In presence of complementary oligonucleotides, the gold particle is released from the QD, regenerating QD fluorescence (Fig. 7b).…”

Section: Review Articlementioning

confidence: 99%

Applications of Nanoparticles in Biology

2008

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Section: Review Articlementioning

confidence: 99%

Applications of Nanoparticles in Biology

2008

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“…In current literature, quenching of QD by metal ions, such as Cu 2þ and Ag þ , and by organic molecules containing amino-groups have been reported. [11][12][13] Influence of various compounds on the fluorescence emission properties of QD raised a strong interest in their use as fluorescent sensors, like the quenching by metal ions, [12,14,15] gold nanoparticles, [16,17] and various other compounds. [18][19][20][21][22][23] Further studies focussed on the oxidation of QD, [24] or photo-induced charge transfer, [11] and also on the influence of experimental conditions, such as temperature or pH.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Quenching of Quantum Dots by DNA Nucleotides and Amino Acids

Siegberg¹,

Herten²

2011

Aust. J. Chem.

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Quantum dots found widespread application in the biosciences as bright and highly photo-stable fluorescent probes, i.e. for single-particle tracking. In this work we used ensemble spectroscopy and single-molecule techniques to study the quenching of quantum dots by various biochemical compounds that are usually present in living cells and might thus influence the experiments. We found not only nucleotides such as cytosine, guanine, and thymine can significantly influence the fluorescence emission of CdSe and CdTe quantum dots, but also amino acids, like asparagine and tryptophan. Bulk studies on fluorescence quenching indicated a static quenching mechanism. Interestingly, we could also show by single-molecule fluorescence spectroscopy that quenching of the quantum dots can be irreversible, suggesting either a redox-reaction between quantum dot and quencher or strong binding of the quencher to the surface of the bio-conjugated quantum dots.

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“…In fact, clever assays for the recognition of various analytes are starting to be designed on the basis of energy transfer processes (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Instead, mechanisms based on electron transfer still remain to be explored (35,36).…”

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confidence: 99%

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Yildiz

Tomasulo

Raymo

2006

Proc. Natl. Acad. Sci. U.S.A.

136

106

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Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.electron transfer ͉ luminescent chemosensors ͉ nanoparticles ͉ proteinligand interactions S emiconductor quantum dots are inorganic nanoparticles with remarkable photophysical properties (1-5). In particular, their one-and two-photon absorption cross-sections, luminescence lifetimes, and photobleaching resistances are significantly greater than those of conventional organic fluorophores. Furthermore, their broad absorption bands extend continuously from the UV to the visible region of the electromagnetic spectrum and, therefore, offer a vast selection of possible excitation wavelengths. Instead, their narrow emission bands can be positioned precisely within the visible and near-infrared regions with fine adjustments of their physical dimensions. In fact, pools of quantum dots with different diameters can be designed to emit in parallel at different wavelengths after excitation at a single wavelength, offering the opportunity to implement unprecedented multichannel assays.Organic dyes do not offer the unique collection of attractive photophysical properties associated with semiconductor quantum dots. Indeed, it is becoming apparent that these inorganic nanoparticles can complement, if not replace, their organic counterparts in a diversity of biomedical applications (6-12). Nonetheless, decades of intensive investigations on the structure and properties of organic chromophores have indicated valuable strategies to design sensitive fluorescent probes able to signal the presence of target analytes with changes in emission intensity (13-16). Their operating principles generally rely on the covalent connection of a fluorescent component to a receptor unit. The receptor is engineered to quench the emission of the fluorophore on the basis of either electron or energy transfer. The supramolecular association of the receptor with a complementary analyte, however, suppresses the quenching mechanism and leads to a si...

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Quenching of CdSe quantum dot emission, a new approach for biosensing

Abstract: The emission of CdSe quantum dots linked to the 5'-end of a DNA sequence is efficiently quenched by hybridisation with a complementary DNA strand with a gold nanoparticle attached at the 3'-end; contact of the quantum dot and gold nanoparticle occurs.

Cited by 206 publications

References 30 publications

Applications of Nanoparticles in Biology

Applications of Nanoparticles in Biology

Fluorescence Quenching of Quantum Dots by DNA Nucleotides and Amino Acids

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

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