Quel cirque, ces virus

Chasseur, Alexis Serge; Muylkens, Benoît; Coupeau, Damien

doi:10.1051/medsci/2023051

Med Sci (Paris)

2023

DOI: 10.1051/medsci/2023051

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Quel cirque, ces virus

Alexis Serge Chasseur

Benoît Muylkens

Damien Coupeau

Abstract: Les ARN circulaires (ARNcirc) font partie intégrante de la relation hôte-pathogène. Après la description de la dérégulation d’ARNcirc cellulaires lors d’infections virales, de nombreux ARNcirc d’origine virale ont été identifiés et caractérisés. Des rôles régulateurs, aussi bien du cycle cellulaire que du cycle viral, leur ont été attribués. Ils sont associés à l’oncogenèse viro-induite, à la régulation du système immunitaire et à la différenciation cellulaire. Ces boucles d’ARN, aussi archaïques que les premi… Show more

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2024

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Unbiased and comprehensive identification of viral encoded circular RNAs in a large range of viral species and families

Chasseur,

Bellefroid,

Galais

et al. 2024

Preprint

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Non-coding RNAs play a significant role in viral infection cycles, with recent attention focused on circular RNAs (circRNAs) originating from various viral families. Notably, these circRNAs have been associated with oncogenesis and alterations in viral fitness. However, identifying their expression has proven more challenging than initially anticipated due to unique viral characteristics. This challenge has the potential to impede progress in our understanding of viral circRNAs. Key hurdles in working with viral genomes include: (1) the presence of repetitive regions that can lead to misalignment of sequencing reads, and (2) unconventional splicing mechanisms that deviate from conserved eukaryotic patterns.To address these challenges, we developed vCircTrappist, a bioinformatic pipeline tailored to identify backsplicing events and pinpoint loci expressing circRNAs in RNA sequencing data. Applying this pipeline, we obtained novel insights from both new and existing datasets encompassing a range of animal and human pathogens belonging to Herpesviridae, Retroviridae, Adenoviridae and Orthomyxoviridae families. Subsequent RT-PCR and Sanger sequencings validated the accuracy of the developed bioinformatic tool for a selection of new candidate viral encoded circRNAs. These findings demonstrate that vCircTrappist is an open and unbiased approach for comprehensive identification of virus-derived circRNAs.Significance StatementCircular RNAs (circRNAs) were revealed to have prominent roles in cellular life in the past decade. They were more recently shown to be expressed by viruses, influencing their infectious cycles and host-pathogen relationship. In this context, viruses that were not previously associated with cellular splicing processes are shown to express circRNAs through unknown mechanisms. These non-canonical circRNAs were already shown to be important in the viral cycle and pathogenesis of the viruses they are encoded from. Here, we propose a bioinformatics pipeline that bypasses the limitations of the existing tools in the identification of viral circRNA. Using this pipeline, we discovered numerous candidates and invite the reader to start its own exploration in the realm of viral encoded circRNAs.Graphical Abstract

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Unbiased and comprehensive identification of viral encoded circular RNAs in a large range of viral species and families

Chasseur,

Bellefroid,

Galais

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

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Quel cirque, ces virus

Cited by 1 publication

References 31 publications

Unbiased and comprehensive identification of viral encoded circular RNAs in a large range of viral species and families

Unbiased and comprehensive identification of viral encoded circular RNAs in a large range of viral species and families

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