Quaternized chitosan-Matrigel-polyacrylamide hydrogels as wound dressing for wound repair and regeneration

Xue, Hang; Hu, Liangcong; Xiong, Yuan; Zhu, Xiangwei; Wei, Congying; Cao, Faqi; Zhou, Wu; Sun, Yun; Endo, Yori; Liu, Mengfei; Yi, Lunzhao; Liu, Jing; Abududilibaier, Abudula; Chen, Lang; Yan, Chenchen; Mi, Bobin; Liu, Guohui

doi:10.1016/j.carbpol.2019.115302

Cited by 185 publications

(109 citation statements)

References 56 publications

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“…Quaternization generally occurs via three methods, namely direct quaternary ammonium substitution, N-alkylation, and the epoxy derivative open loop method [65][66][67][68][69]. In recent years, the most commonly used quaternary ammonium salts have been GTA (2,3-epoxypropyl trimethyl ammonium chloride) and CTA (3-chloro-2-hydroxypropyl trimethyl) [15,70]. N,N,N-trimethyl chitosan (TMC) is a quaternary ammonium chitosan.…”

Section: Quaternary Ammonium Chitosanmentioning

confidence: 99%

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Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

586

326

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Chitosan is a product of the deacetylation of chitin, which is widely found in nature. Chitosan is insoluble in water and most organic solvents, which seriously limits both its application scope and applicable fields. However, chitosan contains active functional groups that are liable to chemical reactions; thus, chitosan derivatives can be obtained through the chemical modification of chitosan. The modification of chitosan has been an important aspect of chitosan research, showing a better solubility, pH-sensitive targeting, an increased number of delivery systems, etc. This review summarizes the modification of chitosan by acylation, carboxylation, alkylation, and quaternization in order to improve the water solubility, pH sensitivity, and the targeting of chitosan derivatives. The applications of chitosan derivatives in the antibacterial, sustained slowly release, targeting, and delivery system fields are also described. Chitosan derivatives will have a large impact and show potential in biomedicine for the development of drugs in future.

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Section: Quaternary Ammonium Chitosanmentioning

confidence: 99%

“…chloro-2-hydroxypropyl trimethyl) [15,70]. N,N,N-trimethyl chitosan (TMC) is a quaternary ammonium chitosan.…”

Section: Quaternary Ammonium Chitosanmentioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

586

326

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“…A desirable wound dressing should have certain antimicrobial functions to minimize pathogens to reduce inflammatory response and tissue fluid exudation at the wound site and accelerate the healing process [32]. Figure 1 shows zones of inhibition of CS, GT, and CS-GT against S. aureus and P. aeruginosa, respectively.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

et al. 2020

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Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing.

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“…Cytokines such as TNF-α, IL-1β, and IL-10 are involved in the wound healing process [65]. During the onset of the inflammatory phase, activated macrophages produce IL-1β and TNF-α, which induce inflammatory leukocyte recruitment into the wounded tissues and stimulate the activity of fibroblasts as well as the synthesis and breakdown of extracellular matrix proteins that are involved in the healing of the injured tissues [66].…”

Section: Cytokine Analysismentioning

confidence: 99%

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

et al. 2020

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Chitosan films entrapped with the Mansoa hirsuta fraction (CMHF) was developed as a new dressing for wound care. The chromatographic profile of the M. hirsuta fraction (MHF) was evaluated by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and the results showed that MHF is rich in acid triterpenes. Physicochemical characterization of the films prepared using the solvent casting method was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetry (TGA), differential scanning calorimetry (DCS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and mechanical properties. CMHF exhibited characteristic bands of both chitosan and MHF, revealing a physical mixture of both. CMHF presented an amorphous nature, thermostability, and dispersion of MHF in the chitosan matrix, resulting in a rough structure. Incorporation of M. hirsuta fraction into chitosan matrix favorably enhanced the mechanical performance and films thickness. The in vivo wound treatment with CMHF for seven days showed a characteristic area of advanced healing, re-epithelization, cell proliferation, and collagen formation. Furthermore, wound closure reached 100% contraction after 10 days of treatment with modulation of interleukins. The incorporation of M. hirsuta fraction into chitosan films was advantageous and showed great potential for stimulating wound repair and regeneration.

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Quaternized chitosan-Matrigel-polyacrylamide hydrogels as wound dressing for wound repair and regeneration

Cited by 185 publications

References 56 publications

Chitosan Derivatives and Their Application in Biomedicine

Chitosan Derivatives and Their Application in Biomedicine

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

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