Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Ryu, Seung Mok; Kwon, Jae‐Young; Seo, Young Hye; Song, Eun Gyeong; Hong, Seong Su; Kim, Beom Seok; Hong, Jin Sung; Ryu, Ki Hyun; Lee, Dong‐Ho

doi:10.1016/j.virusres.2016.09.013

Cited by 14 publications

(15 citation statements)

References 20 publications

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“…Using a leaf-based assay, Ryu et al further investigated the antiviral properties of various quassinoids to inhibit pepper mottle virus (PepMOV), which affects pepper plants (Capsicum spp.) and can impair pepper production quality and yield [58]. It was discovered that a BJO extract and various quassinoids therein, including brucein A, inhibited the PepMOV infection of plant leaves based on a fluorescence reporter assay, in which case PepMOV-infected leaves exhibited fluorescence under ultraviolet (UV) light.…”

Section: Antiviral Activitymentioning

confidence: 99%

Medicinal Activities and Nanomedicine Delivery Strategies for Brucea javanica Oil and Its Molecular Components

et al. 2020

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Brucea javanica oil (BJO) is widely used in traditional Chinese medicine to treat various types of cancer and inflammatory diseases. There is significant interest in understanding the medicinal activities of BJO and its molecular components, especially quassinoids, and in exploring how they can be incorporated into nanomedicine delivery strategies for improved application prospects. Herein, we cover the latest progress in developing different classes of drug delivery vehicles, including nanoemulsions, liposomes, nanostructured lipid carriers, and spongosomes, to encapsulate BJO and purified quassinoids. An introduction to the composition and medicinal activities of BJO and its molecular components, including quassinoids and fatty acids, is first provided. Application examples involving each type of drug delivery vehicle are then critically presented. Future opportunities for nanomedicine delivery strategies in the field are also discussed and considered within the context of translational medicine needs and drug development processes.

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Section: Antiviral Activitymentioning

confidence: 99%

Medicinal Activities and Nanomedicine Delivery Strategies for Brucea javanica Oil and Its Molecular Components

et al. 2020

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“…Quassinoids are phytoconstituents of several Simaroubaceae species and consist of degraded triterpenes. They have demonstrated activity against viral proteins [31, 32] and cancerous cells [33,34]. They have also shown pharmacological action against inflammation, insects and ulcer [31].…”

Section: Resultsmentioning

confidence: 99%

Communication: Theoretical Insights from Binding of Neigleria fowleri Cell Division Proteins with Amoebicidal Quassinoids

Basharat

Zaib

Yasmin

2018

Preprint

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The ameboflagellate Neigleria fowleri, also known as brain eating amoeba is responsible for fatal primary amoebic meningoencephalitis (PAM) infection in humans. Cell division proteins (CDPs) in N. fowleri have been uncharacterized until now, despite their importance in the initiation of cell division and proliferation of the pathogen. Here, we report characterization and structural assembly of eight such proteins associated with division and docking them with anti-amoebic quassinoid compounds. Quassinoids have been implicated as inhibitors of cell proliferation of amoeboid species as well as tumor cells. Here, they were screened computationally to find interaction mechanism as well as binding energies with CDPs of N. fowleri. The identified inhibitors could play a role in prevention of cell division and hence, stop N. fowleri growth and proliferation during infection. This study supports CDPs as a target for anti-amoebic intervention and identifies quassinoid phytochemical compounds as suitable for optimization into a new therapy against N. fowleri.

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“…All treatments were kept at 25 °C for 5 days in the dark. Fluorescence intensity was detected with a FluorCam 7.0 (Photon Systems Instruments, Drasov, Czechia) to quantify the prevalence of TMV 30B …”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence intensity was detected with a FluorCam 7.0 (Photon Systems Instruments, Drasov, Czechia) to quantify the prevalence of TMV 30B. 23 Interference Effect. TMV 30B (100 μL) was agroinfiltrated into N. benthamiana leaves to form a 1 cm diameter circle.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Purification and Characterization of a Secretory Alkaline Metalloprotease with Highly Potent Antiviral Activity from Serratia marcescens Strain S3

Qin

Wang

et al. 2019

J. Agric. Food Chem.

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In this study we report a secretory protein that was purified from Serratia marcescens strain S3 isolated from soil from the tobacco rhizosphere. Subsequent mass spectrometry and annotation characterized the protein as secretory alkaline metalloprotease (SAMP). SAMP plays a crucial role in inhibiting Tobacco mosaic virus (TMV). Transmission electron microscopy (TEM), dynamic light scattering (DLS), confocal microscopy, and microscale thermophoresis (MST) were employed to investigate the anti-TMV mechanism of SAMP. Our results demonstrated that SAMP, as a hydrolytic metal protease, combined and hydrolyzed TMV coat proteins to destroy the virus particles. This study is the first to investigate the antiviral effects of a S. marcescens metalloprotease, and our finding suggests that S. marcescens-S3 may be agronomically useful as a disease-controlling factor active against Tobacco mosaic virus.

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Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Cited by 14 publications

References 20 publications

Medicinal Activities and Nanomedicine Delivery Strategies for Brucea javanica Oil and Its Molecular Components

Medicinal Activities and Nanomedicine Delivery Strategies for Brucea javanica Oil and Its Molecular Components

Communication: Theoretical Insights from Binding of Neigleria fowleri Cell Division Proteins with Amoebicidal Quassinoids

Purification and Characterization of a Secretory Alkaline Metalloprotease with Highly Potent Antiviral Activity from Serratia marcescens Strain S3

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