Quasi-HKUST-1 Nanostructures with Enhanced Catalytic Activity and Water Stability for Bacteria-Infected Diabetic Wound Therapy

Abstract

Metal−organic frameworks (MOFs), a type of nanomaterial, are generating increasing interest in mimicking the structure and function of natural enzymes for sensors and therapeutics. Their catalytic and therapeutic efficiencies, however, are subpar due to their limited catalytic activity and structural stability. Quasi-MOFs (Q-MOFs) exhibit improved metal−carrier interactions and provide synergistic catalysis by exposing inorganic nodes to the object species while retaining a portion of the porous structure of MOFs. In this work, a quasi-Cu-based metal−organic framework was designed to improve bacteria-infected diabetic wound therapy. A series of quasi-HKUST-1 (QHKUST-1) that were created from the low-temperature calcination of HKUST-1 were used for the initial catalytic reaction. Then, the optimal QHKUST-1 has enhanced peroxidase activity to catalyze H 2 O 2 to generate hydroxyl groups for antibacterial therapy. Comparing the previously watersensitive HKUST-1 to the enhanced moisture stability and catalytic capabilities of QHKUST-1. Furthermore, cell migration experiments demonstrate that QHKUST-1 can promote cell migration more effectively than HKUST-1. Finally, animal experiments indicated that QHKUST-1 can effectively facilitate bacteria-infected diabetic wound healing with good antibacterial activity and biocompatibility. This work highlights the potential of Q-MOF for antibacterial therapy and shows significant promise for the future management of bacterially infected wounds.

Copper(ii)-MOFs for bio-applications

“…103,104 Currently, it has been proposed that the antibacterial mechanisms of MOFs are the same as the mechanisms of metal ions, mainly in effect as the production of reactive species (ROS), the change in ionic balance, the destruction or damage of the cell wall, the interaction with thiol groups of the protein, inactivation of important proteins, etc. 98,103,105 This effect was corroborated in 2022 by Chen et al, 106 which reports the observation of morphological alterations in methicillinresistant S. aureus and E. coli in the presence of HKUST-1, see Fig. 4.…”

“…113 In the aspect of evaluation at the site of action, it can be mentioned that there are reports of the in vivo application of these MOF-Cu systems. Guo et al show the improved efficiency for wound healing by applying a MOF-Cu, as an antibacterial alternative with a long-acting effect and in vivo healing of wounds infected by bacteria 106,111 and in vivo wound evaluations have also been reported in mice. 119,120 In this same concept of application at the site of action, but now on inanimate surfaces, we can mention the use of copper-based MOFs in biofilms, for which Wang proposes a study with a dye called SYTO-9, which stains viable bacteria green and dead bacteria red with a dye called PI tested in biofilms.…”

“…Quasi-MOFs called QHKUST-1 obtained from low-temperature calcination of MOFs exhibit enhanced metal-host interactions and provide synergistic catalysis by exposing inorganic nodes, also maintaining a part of the porous structure of MOFs and can effectively facilitate healing. 128 On the other hand, Xiao et al in 2018 designed a hydrogel of MOF and MOF with folic acid, to accelerate wound healing in diabetic mice. These HKUST-1 hydrogels with folic acid showed significantly accelerated wound healing with increased safety due to their ability to release controlled copper ions, which promote angiogenesis, collagen deposition, and wound re-epithelialization.…”

Supported AgCuCl Nanoclusters as Bimetal Catalysts for Propylene Epoxidation with Molecular Oxygen

“…Stability is the key factor of catalytic reaction and needs further improvement . To achieve this, the stability of AgCuCl/BaCO 3 and AgCuCl/BaCO 3 -N 2 was evaluated for 800 min at 275 °C (Figures S7 and c).…”

“…Stability is the key factor of catalytic reaction and needs further improvement. 35 To achieve this, the stability of AgCuCl/BaCO 3 and AgCuCl/BaCO 3 -N 2 was evaluated for 800 min at 275 °C (Figures S7 and 4c). For AgCuCl/BaCO 3 -N 2 , when the reaction time was extended to 75 min, the PO selectivity was increased from 82.23% to 85.29%.…”