Quartz crystal microbalance: Sensing cell-substrate adhesion and beyond

Chen, Jennifer Y.; Penn, Lynn S.; Xi, Jun

doi:10.1016/j.bios.2017.08.032

Cited by 116 publications

(74 citation statements)

References 137 publications

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“…So far, the most successful tool to tackle the challenge of exploring the real-time kinetics of cell adhesion has been the application of surface sensitive label-free methods (surface plasmon resonance (SPR) 29,30 , infrared SPR [31][32][33] , photonic crystals [34][35][36] , quartz crystal microbalance [37][38][39] , ellipsometry 40,41 , digital holographic microscopy 42,43 ), most of them are using surface bound evanescent waves. For example, the resonant waveguide grating (RWG) based optical biosensors measure the local refractive index change in the 150 nm vicinity of the sensor surface, thus providing real-time kinetic information on cell adhesion and spreading, typically on a population of cells (Fig.…”

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confidence: 99%

Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Sztilkovics

Gerecsei

Péter

et al. 2020

Sci Rep

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Single-cell adhesion force plays a crucial role in biological sciences, however its in-depth investigation is hindered by the extremely low throughput and the lack of temporal resolution of present techniques. While atomic force microcopy (AFM) based methods are capable of directly measuring the detachment force values between individual cells and a substrate, their throughput is limited to few cells per day, and cannot provide the kinetic evaluation of the adhesion force over the timescale of several hours. In this study a high spatial and temporal resolution resonant waveguide grating based label-free optical biosensor was combined with robotic fluidic force microscopy to monitor the adhesion of living cancer cells. In contrast to traditional fluidic force microscopy methods with a manipulation range in the order of 300-400 micrometers, the robotic device employed here can address single cells over mm-cm scale areas. This feature significantly increased measurement throughput, and opened the way to combine the technology with the employed microplate-based, large area biosensor. After calibrating the biosensor signals with the direct force measuring technology on 30 individual cells, the kinetic evaluation of the adhesion force and energy of large cell populations was performed for the first time. We concluded that the distribution of the single-cell adhesion force and energy can be fitted by lognormal functions as cells are spreading on the surface and revealed the dynamic changes in these distributions. The present methodology opens the way for the quantitative assessment of the kinetics of single-cell adhesion force and energy with an unprecedented throughput and time resolution, in a completely non-invasive manner.

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mentioning

confidence: 99%

Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Sztilkovics

Gerecsei

Péter

et al. 2020

Sci Rep

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“…Moreover, we tested the stability of the electrostatic binding by real-time QCM-analysis, based on measurements of small mass changes on the sensor surface in the nanogram range with a sensitivity for the applied system of 0.87 ng Hz −1 [21]. The QCM approach has meanwhile evolved from a simple acoustic based mass sensor to a powerful label-free bioanalytical tool [37]. QCM with dissipation detection (QCM-D) uses two independent quantifiable signals, the oscillation frequency and the energy dissipation.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

et al. 2020

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Extensive efforts were undertaken to develop suitable biomaterials for tissue engineering (TE) applications. To facilitate clinical approval processes and ensure the success of TE applications, bioinspired concepts are currently focused on. Working on bone tissue engineering, we describe in the present study a method for biofunctionalization of collagen/hydroxyapatite composites with BMP-2 mimetic peptides. This approach is expected to be fundamentally transferable to other tissue engineering fields. A modified BMP-2 mimetic peptide containing a negatively charged poly-glutamic acid residue (E7 BMP-2 peptide) was used to bind positively charged hydroxyapatite (HA) particles by electrostatic attraction. Binding efficiency was biochemically detected to be on average 85% compared to 30% of BMP-2 peptide without E7 residue. By quartz crystal microbalance (QCM) analysis, we could demonstrate the time-dependent dissociation of the BMP-2 mimetic peptides and the stable binding of the E7 BMP-2 peptides on HA-coated quartz crystals. As shown by immunofluorescence staining, alkaline phosphatase expression is similar to that detected in jaw periosteal cells (JPCs) stimulated with the whole BMP-2 protein. Mineralization potential of JPCs in the presence of BMP-2 mimetic peptides was also shown to be at least similar or significantly higher when low peptide concentrations were used, as compared to JPCs cultured in the presence of recombinant BMP-2 controls. In the following, collagen/hydroxyapatite composite materials were prepared. By proliferation analysis, we detected a decrease in cell viability with increasing HA ratios. Therefore, we chose a collagen/hydroxyapatite ratio of 1:2, similar to the natural composition of bone. The following inclusion of E7 BMP-2 peptides within the composite material resulted in significantly elevated long-term JPC proliferation under osteogenic conditions. We conclude that our advanced approach for fast and cost-effective scaffold preparation and biofunctionalization is suitable for improved and prolonged JPC proliferation. Further studies should prove the functionality of composite scaffolds in vivo.

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“…Interfacial properties of solid-liquid interface have to be considered [79]. Nevertheless, QCMs are used for biosensing [80,81] and even cell behavior is examined [82]. Picomolar specific biomarker target detection was achieved for miss-matches of non-coding RNA [83] or using micro-cantilever arrays for early liver cancer diagnosis [84].…”

Section: Biomolecular Interaction Analysismentioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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Direct optical detection has proven to be a highly interesting tool in biomolecular interaction analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis, clinical diagnostics, screening of large data volumes in immunology, cancer therapy, or personalized medicine. In this review, the fundamental optical principles and applications are reviewed. Devices are based on concepts such as refractometry, evanescent field, waveguides modes, reflectometry, resonance and/or interference. They are realized in ring resonators; prism couplers; surface plasmon resonance; resonant mirror; Bragg grating; grating couplers; photonic crystals, Mach-Zehnder, Young, Hartman interferometers; backscattering; ellipsometry; or reflectance interferometry. The physical theories of various optical principles have already been reviewed in detail elsewhere and are therefore only cited. This review provides an overall survey on the application of these methods in direct optical biosensing. The "historical" development of the main principles is given to understand the various, and sometimes only slightly modified variations published as "new" methods or the use of a new acronym and commercialization by different companies. Improvement of optics is only one way to increase the quality of biosensors. Additional essential aspects are the surface modification of transducers, immobilization strategies, selection of recognition elements, the influence of non-specific interaction, selectivity, and sensitivity. Furthermore, papers use for reporting minimal amounts of detectable analyte terms such as value of mass, moles, grams, or mol/L which are difficult to compare. Both these essential aspects (i.e., biochemistry and the presentation of LOD values) can be discussed only in brief (but references are provided) in order to prevent the paper from becoming too long. The review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

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Quartz crystal microbalance: Sensing cell-substrate adhesion and beyond

Cited by 116 publications

References 137 publications

Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

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