Quartz crystal microbalance (QCM) as biosensor for the detecting of Escherichia coli O157:H7

Abstract

Although Escherichia coli (E. coli) is a commensalism organism in the intestine of humans and warm-blooded animals, it can be toxic at higher density and causes diseases, especially the highly toxic E. coli O157:H7. In this paper a quartz crystal microbalance (QCM) biosensor was developed for the detection of E. coli O157:H7 bacteria. The anti-E. coli O157:H7 antibodies were immobilized on a self-assembly monolayer (SAM) modified 5 MHz AT-cut quartz crystal resonator. The SAMs were activated with 16-mercaptopropanoic acid, in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and ester N-hydroxysuccinimide (NHS). The result of changing frequency due to the adsorption of E. coli O157:H7 was measured by the QCM biosensor system designed and fabricated by ICDREC-VNUHCM. This system gave good results in the range of 102–107 CFU mL−1 E. coli O157:H7. The time of bacteria E. coli O157:H7 detection in the sample was about 50 m. Besides, QCM biosensor from SAM method was comparable to protein A method-based piezoelectric immunosensor in terms of the amount of immobilized antibodies and detection sensitivity.

Electrospun Nanofibers for Quartz Crystal Microbalance Gas Sensors: A Review

“…When being used as a sensor, the device frequency will be altered in response to the additional mass of adsorbed target analytes or molecules. Because of their high sensitivity and stability in a liquid environment, QCM platforms have been widely used not only for detecting chemical substances in the air (e.g., volatile organic compounds (VOCs), toxic gases, and water vapors), − but also for sensing biological objects in liquid (e.g., breast cancer cells, Escherichia coli , Salmonella typhimurium − ). Moreover, by integrating an electronic data acquisition system and artificial intelligence with a QCM sensor array, an intelligent mobile electronic nose was developed to perform reliable qualitative and quantitative analyses of complex gaseous mixtures consisting of different VOCs …”

Current Trends on Surface Acoustic Wave Biosensors

“…Their biosensor had shown a detection sensitivity to be at 0.6439 × 10 −9 m /0.1 kHz as well as an excellent detection limit of 1.8 × 10 −15 m which was superior compared to previous works on SAW biosensors for E. coli O157:H7 detection. [ 181,182 ]…”

Advances in Research on Biomedical Nanomaterials in Vietnam

“…In reference Ngo V K T et al [17] demonstrated a quartz crystal microbalance (QCM) as biosensor for detecting E. coli O157:H7. The E. coli O157:H7 antibodies were immobilized on a self-assembly monolayer (SAM) modified 5 MHz AT-cut quartz crystal resonator.…”