We consider the effects of interactions on spinon excitations in Heisenberg spin-1/2 chains. We compute the exact two-spinon part of the longitudinal structure factor of the infinite chain in zero field for all values of anisotropy in the gapless antiferromagnetic regime, via an exact algebraic approach. Our results allow us to quantitatively describe the behaviour of these fundamental excitations throughout the observable continuum, for cases ranging from free to fully coupled chains, thereby explicitly mapping the effects of 'turning on the interactions' in a strongly-correlated system.Interactions in one-dimensional (1d) systems are known to overwhelm constituent particles, leading to a collective quantum liquid state with low-energy excitations described by the theory of Tomonaga-Luttinger liquids 1 . While the 'universal' physics of 1d systems is phenomenologically well understood 2 , it almost always remains impossible to precisely track the effects of 'turning on the interactions' on the constituent particles, as one does for Fermi liquids 3 (where bare fermions are adiabatically connected to Landau quasiparticles). In this respect, our general understanding of 1d systems can benefit from nonperturbative solutions of microscopic models, a fundamental example being the Heisenberg spin-1/2 anisotropic chain 4,5 , whose Hamiltonian isThis system is a Tomogana-Luttinger liquid for anisotropy values ∆ in the range −1 < ∆ ≤ 1 (in zero field, with J > 0). Its fundamental excitations are spinons 6 : spin-1/2 fractionalized objects which can be viewed as domain walls dressed by quantum fluctuations.A way to probe the nature of excitations is to determine how they carry observable correlations, an interesting example here being the longitudinal structure factorAt ∆ = 0, this can be written as a density correlator of Jordan-Wigner fermions. Only single particle-hole excitations contribute, the exact structure factor being proportional to their density of states. For ∆ > 0, this picture breaks down 7,8 due to nonperturbative effects. It is the purpose of this paper to track in detail the effects of 'turning on' interactions on the spinon quasiparticles and their ability to carry correlations, throughout the gapless antiferromagnetic regime 0 ≤ ∆ ≤ 1. Systems in this regime can be realized and studied experimentally (for fixed anisotropy) in spin ladder compounds 9-11 or (in principle for generic anisotropy) using optical lattices 12 . Focusing on zero temperature, we will compute the exact two-spinon contribution to (2) directly in the thermodynamic limit N → ∞, using an adaptation of the 'vertex operator approach' 13 . Our results provide a strict lower bound and (for practical purposes) an extremely accurate representation for the complete correlator of the infinite system (more that 99% for anisotropies below 0.5) throughout the observable excitation continuum. They provide a robust benchmark for assessing the lineshapes obtained for finite systems directly from integrability 14,15 or using variants of the density...