Quantum-enhanced stimulated Raman scattering microscopy in a high-power regime

Xu, Zicong; Oguchi, Kenichi; Taguchi, Yoshiharu; Takahashi, Shogo; Sano, Yuki; Mizuguchi, Takaha; Katoh, Kazuhiro; Ozeki, Yasuyuki

doi:10.1364/ol.473130

Cited by 19 publications

(12 citation statements)

References 20 publications

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“…Figure 6. Single point SRS spectra of shot-noise limit balanced detection SRS and QE-SRS acquired with RFSA 15 . 0 dB level is determined by shot-noise level without SRS signal.…”

Section: Resultsmentioning

confidence: 99%

“…Compared with Figs. 7 (a 15 . The upper side region colored with blue shows d8-PS, and the lower side colored with yellow shows d6-DMSO.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2. A detailed schematic of QE-SRS system including feedback system 15 . PBS, polarization-sensitive beamsplitter; SHG, second-harmonic generation; OPA, optical parametric amplification; AOM, acousto-optic modulator; DM, dichroic mirror; OBJ, objective lens; PD, photodiode; SLM, spatial light modulator; HWP, half-wave plate; SPF, optical short pass filter; G, grating; GM, galvanometric mirror; YDFA, Yb-doped fiber amplifier; RFSA, radio-frequency spectrum analyzer; LIA, lock-in amplifier; DAQ, data acquisition system; PC, personal computer.…”

Section: Feedback Control System For Qe-srsmentioning

confidence: 99%

“…To overcome shot-noise-limited sensitivity of SRS microscopy, quantum-enhanced (QE-) SRS microscopy is expected as a promising technique [12][13][14][15] . R. B. de Andrade et al demonstrated QE-SRS by using continuous light source, where continuous light was mixed with squeezed vacuum at asymmetric beamsplitter, and used as SRS Stokes light.…”

Section: Introductionmentioning

confidence: 99%

“…In this paper, we demonstrate QE-SRS microscopy with high-power squeezed pulses as SRS pump pulses by using quantum-enhanced balanced detection (QE-BD) scheme [14][15][16] , where picosecond squeezed vacuum is injected to a balanced detection SRS microscope to cancel out the shot noise. QE-BD scheme is advantageous to realize QE-SRS with highpower SRS pump pulse (several tens of milliwatt), which is often utilized in SRS microscopy for cell and tissue imaging.…”

Section: Introductionmentioning

confidence: 99%

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Quantum-enhanced stimulated Raman scattering microscopy toward breaking the shot noise limit in high-power regime

Oguchi

Taguchi

et al. 2023

Advanced Chemical Microscopy for Life Science and Translational Medicine 2023

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Stimulated Raman scattering (SRS) microscopy is a powerful technique that allows for the visualization of molecular vibrational images for label-free imaging, metabolic imaging, and supermultiplex imaging. However, its sensitivity is mainly limited by the shot noise of laser pulses, and long acquisition times are sometimes required to detect weak SRS signals hidden by the shot noise. To overcome this limitation, quantum-enhanced (QE-) SRS microscopy has been demonstrated, while the low optical power of squeezed pulses limits the sensitivity. In this work, we present QE-SRS microscopy using quantum-enhanced balanced detection (QE-BD) scheme, where the sensitivity of balanced detection SRS microscope is enhanced by injecting squeezed vacuum, allowing for QE-SRS imaging with high power SRS pump pulses (typically several tens of milliwatt), while balanced detection causes 3 dB drawback in the signal-to-noise ratio. We experimentally demonstrate QE-SRS imaging with 2.6 dB noise reduction compared with shot-noise-limited balanced detection SRS. We also demonstrate hyperspectral QE-SRS imaging by fast wavelength tuning of Stokes pulses. These results show the potential feasibility of high-power QE-SRS whose sensitivity is beyond that of classical shot-noise-limited SRS microscopes.

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“…Figure 6. Single point SRS spectra of shot-noise limit balanced detection SRS and QE-SRS acquired with RFSA 15 . 0 dB level is determined by shot-noise level without SRS signal.…”

Section: Resultsmentioning

confidence: 99%

“…Compared with Figs. 7 (a 15 . The upper side region colored with blue shows d8-PS, and the lower side colored with yellow shows d6-DMSO.…”

Section: Resultsmentioning

confidence: 99%

Section: Feedback Control System For Qe-srsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantum-enhanced stimulated Raman scattering microscopy toward breaking the shot noise limit in high-power regime

Oguchi

Taguchi

et al. 2023

Advanced Chemical Microscopy for Life Science and Translational Medicine 2023

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Visualization of Modified Bisarylbutadiyne-Tagged Small Molecules in Live-Cell Nuclei by Stimulated Raman Scattering Microscopy

Kawaguchi,

Yonetani,

Mizuguchi

et al. 2024

Anal. Chem.

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Visualizing the distribution of small-molecule drugs in living cells is an important strategy for developing specific, effective, and minimally toxic drugs. As an alternative to fluorescence imaging using bulky fluorophores or cell fixation, stimulated Raman scattering (SRS) imaging combined with bisarylbutadiyne (BADY) tagging enables the observation of small molecules closer to their native intracellular state. However, there is evidence that the physicochemical properties of BADY-tagged analogues of small-molecule drugs differ significantly from those of their parent drugs, potentially affecting their intracellular distribution. Herein, we developed a modified BADY to reduce deviations in physicochemical properties (in particular, lipophilicity and membrane permeability) between tagged and parent drugs, while maintaining high Raman activity in live-cell SRS imaging. We highlight the practical application of this approach by revealing the nuclear distribution of a modified BADY-tagged analogue of JQ1, a bromodomain and extra-terminal motif inhibitor with applications in targeted cancer therapy, in living HeLa cells. The modified BADY, methoxypyridazyl pyrimidyl butadiyne (MPDY), revealed intranuclear JQ1, while BADY-tagged JQ1 did not show a clear nuclear signal. We anticipate that the present approach combining MPDY tagging with live-cell SRS imaging provides important insight into the behavior of intracellular drugs and represents a promising avenue for improving drug development.

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