Quantum Dots for Human Mesenchymal Stem Cells Labeling. A Size-Dependent Autophagy Activation

Seleverstov, Oleksandr; Zabirnyk, Olga; Zscharnack, Matthias; Bulavina, Larysa; Nowicki, Marcin; Heinrich, Jan-Michael; Yezhelyev, Maksym V.; Emmrich, Frank; O'Regan, Ruth; Bader, Augustinus

doi:10.1021/nl0619711

Cited by 238 publications

(189 citation statements)

References 36 publications

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“…As one cell receives most of the particles, this cell is likely 'sacrificed' so that the remaining daughter cells with nearly no particles have a better chance to survive. Cellular responses to stress induced by nanosized materials can also lead to the induction of autophagy, a self-defense mechanism which can occur in all eukaryotic cells which contain lysosomes [91]. Typically, two membranes will be formed surrounding the region where the nanoparticles are located, to separate this region from the rest of the cytoplasm and then fuse with lysosomes in order to reduce cellular stress and degrade the particles [91].…”

Section: Mitotic Partitioning Of Inorganic Quantum Dots Gold and Iromentioning

confidence: 99%

“…Cellular responses to stress induced by nanosized materials can also lead to the induction of autophagy, a self-defense mechanism which can occur in all eukaryotic cells which contain lysosomes [91]. Typically, two membranes will be formed surrounding the region where the nanoparticles are located, to separate this region from the rest of the cytoplasm and then fuse with lysosomes in order to reduce cellular stress and degrade the particles [91]. Also gold nanoparticles, which are generally seen as quite biocompatible, induce oxidative stress upon cellular internalization which in turn activates autophagy [92].…”

Section: Mitotic Partitioning Of Inorganic Quantum Dots Gold and Iromentioning

confidence: 99%

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Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Symens

Soenen

Rejman

et al. 2012

Advanced Drug Delivery Reviews

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Section: Mitotic Partitioning Of Inorganic Quantum Dots Gold and Iromentioning

confidence: 99%

Section: Mitotic Partitioning Of Inorganic Quantum Dots Gold and Iromentioning

confidence: 99%

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Symens

Soenen

Rejman

et al. 2012

Advanced Drug Delivery Reviews

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“…3 There are many examples in the literature of nanoscale particulates, such as quantum dots, inducing elevated levels of autophagic vacuoles in both animal and human cell culture models. [4][5][6][7][8] Such nanomaterials include: nanoscale carbon black, fullerene and fullerene derivatives, nanoscale neodymium oxide, and protein-coated quantum dots. The induction of autophagic vacuoles in these instances was characterized at the ultrastructural cellular level via electron microscopy detection of vacuoles in treated cells, through immunoblot detection of LC3-I to LC3-II conversion (an established marker of autophagy activity) in cell lysates, and/or cellular immunolabeling of punctate LC3-II autophagosome incorporation.…”

Section: Introductionmentioning

confidence: 99%

Role for nanomaterial-autophagy interaction in neurodegenerative disease

Stern

Johnson²

2008

Autophagy

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Nanotechnology is the control and manipulation of materials in the size range of 1-100 nm. Due to increasing research into the potential beneficial applications of nanotechnology, there is an urgent need for the study of possible health risks. Several researchers, including those in our laboratory, have demonstrated elevated levels of autophagic vacuoles upon exposure of cells to certain nanomaterials, including carbon-and metal-based nanoparticles. While this apparent increase in autophagic activity may be an appropriate cellular response toward nanomaterial clearance, often the interaction between nanomaterials and the autophagy pathway is disruptive, resulting in severe morphological changes and coincident cell death. Interestingly, epidemiological studies have identified an association between exposure to combustion-derived ambient particles (which are predominantly nanoscale) and neurological conditions with Alzheimer's and Parkinson's disease-like pathologies. Becuse impaired autophagy may play an important role in the pathogenesis of these and other diseases, it is intriguing to speculate about the plausible involvement of nanoscale particulates in this process. The interaction of nanomaterials with the autophagy pathway, and the potential negative consequences of resulting autophagy dysfunction, should be explored further.

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“…Seleverstov et al [79] studied the process of internalization and compared the cytotoxicity of two different-sized QDs (605 and 525 nm) in the human mesenchymal stem cells (hMSC). In the cells treated with QDs of smaller size (QD525), they observed autophagic cell death and the massive formation of autophagosomes containing damaged organelles and aggregated QDs after 72 h. In contrast, little autophagic vacuoles or damaged mitochondria were found in hMSC cells labeled with QDs of bigger size (QD605).…”

Section: Semiconductor Quantum Dotsmentioning

confidence: 99%

Autophagy as new emerging cellular effect of nanomaterials

et al. 2013

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The biosafety issue of nanoscale materials is getting more and more attention with their increasing manufacture and application. In the research of cellular effects and underlying mechanisms related to toxicity of nanomaterials, most emphasis were placed on processes such as apoptosis, metabolic inhibition and oxidative stress. Recent evidence suggests that autophagy is part of the biological effects by nanomaterials and various kinds of nanomaterials are capable of disturbing the autophagic process. This review will highlight the importance of autophagy as an emerging mechanism of nanomaterial toxicity and the implication in the therapy of autophagy-related diseases. We summarize current research status of interaction between nanomaterials and autophagic pathways. It is of note that nanomaterials can either induce or block autophagy, which result in similar phenotype but completely different biological consequence. It is therefore important to perform comprehensive analysis of the whole autophagic flux in the future research. Nanomaterials refer to materials with the size range between 1 and 100 nanometers in one or more dimensions [1]. Due to their superior physicochemical properties, such as ultra small size, increased ratio of surface area to volume, multiple options for modification and relatively good biocompatibilities, application of nanomaterials in research as well as in industry becomes more and more common. It is predicted that the total amount of nanomaterials' production will increase up to 58000 tons by 2020. Particularly, the utilization of nanomaterials in biomedical research as biosensors, drug-carriers, or imaging agents was extensively studied [2][3][4]. Large scaled preparation and application of nanomaterials increase their possibilities to be exposed to human beings or enter the environment [5][6][7]. Therefore the impact of nanomaterials on environment and human health has attracted high attention. Related studies were carried out on the biological effect of various kinds of nanomaterials both in vitro and in vivo [8]. To date, reported physiological changes of a cell or an organism that exposed to nanomaterials include: reactive oxygen species (ROS)-related pathological responses (such as damaged membrane, mitochondrial damage and necrosis), apoptosis, inflammation and altered differentiation patterns, etc [9][10][11][12]. Recently, autophagy as an essential cellular process and a novel biological effect of nanomaterials on eukaryotes, has received much attention. Many nanomaterials were reported to be able to change the basal level of autophagy [13,14]. Autophagy can crosstalk with other cellular process, such as apoptosis and necrosis, and therefore is an important part of the cellular effect of nanomaterials [15,16]. Moreover, nanomaterials-induced autophagy showed potential for the treatment of certain diseases including neurodegenerative diseases and cancer [13,17]. Hence, in this review we summarize the current research on the effect of various kinds of nanomaterials on autophagy. ...

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Quantum Dots for Human Mesenchymal Stem Cells Labeling. A Size-Dependent Autophagy Activation

Cited by 238 publications

References 36 publications

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Role for nanomaterial-autophagy interaction in neurodegenerative disease

Autophagy as new emerging cellular effect of nanomaterials

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