Quantum Dots CdSe/ZnS-Loaded Poly(D,L-Lactide-Co-Glycolide) Nanoparticles: Physicochemical Characterization and Application

Yang, Chih Hui; Lin, Kuo Chin; Chang, Yu Huai; Lin, Yu‐Cheng

doi:10.4028/0-87849-990-3.667

Cited by 1 publication

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“…The authors show homogenous distribution of NCs covering PLGA particle surface by TEM and are able to detect fluorescent PLGA nanoparticles by fluorescence microscopy. Other efforts illustrate the possibility to encapsulate NCs into PLGA particles using the nanoprecipitation method [30,31]. In this report, we introduce a novel protocol to efficiently encapsulate NCs into PLGA-MS using the spray drying method.…”

Section: Discussionmentioning

confidence: 99%

Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy

Schliehe

Thiry

et al. 2011

Journal of Controlled Release

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Biodegradable poly-(D,L-lactide-co-glycolide) microspheres (PLGA-MS) are approved as a drug delivery system in humans and represent a promising antigen delivery device for immunotherapy against cancer. Immune responses following PLGA-MS vaccination require cross-presentation of encapsulated antigen by professional antigen presenting cells (APCs). While the potential of PLGA-MS as vaccine formulations is well established, the intracellular pathway of cross-presentation following phagocytosis of PLGA-MS is still under debate. A part of the controversy stems from the difficulty in unambiguously identifying PLGA-MS within cells. Here we show a novel strategy for the efficient encapsulation of inorganic nanocrystals (NCs) into PLGA-MS as a tool to study their intracellular localization. We microencapsulated NCs as an electron dense marker to study the intracellular localization of PLGA-MS by transmission electron microscopy (TEM) and as fluorescent labels for confocal laser scanning microscopy. Using this method, we found PLGA-MS to be rapidly taken up by dendritic cells and macrophages. Co-localization with the lysosomal marker LAMP1 showed a lysosomal storage of PLGA-MS for over two days after uptake, long after the initiation of cross-presentation had occurred. Our data argue against an escape of PLGA-MS from the endosome as has previously been suggested as a mechanism to facilitate cross-presentation of PLGA-MS encapsulated antigen.

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Section: Discussionmentioning

confidence: 99%