Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol

Yao, Xun; Ping, Yan; Tang, Qinghui; Deng, Anping; Li, Jianguo

doi:10.1016/j.aca.2013.08.029

Cited by 46 publications

(33 citation statements)

References 34 publications

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“…Figure (Figure 4). According to the above 243 explanation and our previous study, 39 the ECL processes could be expressed below: …”

Section: Introductionmentioning

confidence: 97%

“…14 Therefore, it is urgent to establish a simple, rapid and inexpensive 37 approach for monitoring mercury residues and pollutions. 38 Compared with above methods, electrochemiluminescence (ECL) would be a 39 promising method on account of its advantages of simple sample preparation process, 40 high sensitivity and low cost. Usually, the ECL signals can be obtained from the 41 luminophores such as Ru(bpy) 3 2+ , luminol, semiconductor nanocrystals and so on.…”

Section: Introductionmentioning

confidence: 99%

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Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg²⁺ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Cai

Zhu

Zhao

et al. 2015

Environ. Sci. Technol.

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A multiple signal amplification strategy was designed for an ultrasensitive competitive immunoassay for Hg(2+). This strategy was achieved using graphene conjugated with a large number of CdSe quantum dots to enhance the basal signal and enormous horseradish peroxidase (HRP) labeled with gold nanoparticles (AuNPs) to consume the coreactant H2O2 generated in situ. The immunosensor was constructed by immobilization of coating antigen on poly(diallyldimethylammonium chloride)-graphene-CdSe composites (PDDA-GN-CdSe), and a strong electrochemiluminescence (ECL) signal was obtained. When the immunosensor was immersed in antibody-AuNPs-HRP composites, the ECL signal greatly decreased, which was ascribed to the bound enzyme on the electrode surface. The self-produced coreactant H2O2 was consumed by o-phenylenediamine in the presence of enzyme, effectively decreasing the ECL intensity from the quantum dots. The Hg(2+) in solution and the corresponding coating antigen competed for the limited antibody, and thus, the ECL intensity was linearly dependent on the logarithm of the mercury(II) concentration from 0.2 to 1000 ng mL(-1) with a detection limit of 0.06 ng mL(-1). The immunoassay exhibited good stability and accuracy and acceptable reproducibility, indicating that it provides a promising approach for the detection of trace mercury and other small molecular compounds in environmental samples.

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“…Figure (Figure 4). According to the above 243 explanation and our previous study, 39 the ECL processes could be expressed below: …”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg²⁺ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Cai

Zhu

Zhao

et al. 2015

Environ. Sci. Technol.

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“…At present, chromatographic analysis and immunoassays are the mainstream technologies for confirmation and screening of β-agonists in animal feed and animal products [7]. However, the various chromatographic methods reported, such as high-performance liquid chromatography (HPLC) [8,9], gas chromatography-mass spectrometry (GC-MS) [10,11], ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS) [12,13] and capillary electrophoresis (CE) [14,15] are not suitable for field analysis because of the requirement of sophisticated instruments and complicated sample pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Expression of Codon Optimized ��2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of ��-Agonist Residues in Pork

Liu

Wang

Liu

et al. 2019

Journal of Microbiology and Biotechnology

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2-AR) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of β-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and 59.57 μg/l, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was 3.2 μg/l and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified β 2-AR would have great application potential in detection of β-agonist residues.

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“…In particular, the electrochemiluminescence (ECL) systems based on QDs have been extensively studied and applied in recognition and detection in biometry [19][20][21][22]. To obtain excellent selectivity, a monoclonal antibody that binds specifically to mercuric ions was a creative mode.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive electroluminescence immunoassay for Hg(II) ions based on the use of CdSe quantum dots, the methylmercury-6-mercaptonicotinic acid-ovalbumin conjugate, and a specific monoclonal antibody

et al. 2014

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We have designed a rapid and ultrasensitive electrochemiluminescent (ECL) competitive immunoassay for the determination of mercury(II) ion. It is based on the use of CdSe quantum dots (QDs), methylmercury-6-mercaptonicotinic acid-ovalbumin as coating antigen and specific monoclonal antibodies (mAbs) against Hg(II). The latter is quite selective for Hg(II). The coating antigen was immobilized on the surface of a gold electrode via reaction between the functional groups of cysteamine and glutaraldehyde. The mercury(II) ions in a sample and the coating antigen compete for binding sites of QD-labeled monoclonal antibody which binds specifically to Hg(II) ions. The ECL of the system decreases with increasing concentration of Hg(II) because less QD-labeled mAbs are present on the surface of the electrode. Under optimal conditions, the decrease of ECL intensity is linearly related to the logarithm of the Hg(II) concentration in the range from 0.02 to 100 ng mL −1 , with a detection limit of 6.2 pg mL −1 . As far as we know, this is the first report on an ECL immunoassay for Hg(II) based on a specific monoclonal antibody. The favorable results obtained when this method was applied to real samples indicate that this detection scheme can widely enlarge the applicability of detecting heavy metal ions by exploiting the ECL of QDs for immunoassays.

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Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol

Cited by 46 publications

References 34 publications

Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg²⁺ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg²⁺ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Expression of Codon Optimized ��2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of ��-Agonist Residues in Pork

Highly sensitive electroluminescence immunoassay for Hg(II) ions based on the use of CdSe quantum dots, the methylmercury-6-mercaptonicotinic acid-ovalbumin conjugate, and a specific monoclonal antibody

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Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol

Cited by 46 publications

References 34 publications

Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg2+ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg2+ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Expression of Codon Optimized ��2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of ��-Agonist Residues in Pork

Highly sensitive electroluminescence immunoassay for Hg(II) ions based on the use of CdSe quantum dots, the methylmercury-6-mercaptonicotinic acid-ovalbumin conjugate, and a specific monoclonal antibody

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Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg²⁺ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase

Multiple Signal Amplified Electrochemiluminescent Immunoassay for Hg²⁺ Using Graphene-Coupled Quantum Dots and Gold Nanoparticles-Labeled Horseradish Peroxidase