Quantum dots assembly enhanced and dual-antigen sandwich structured lateral flow immunoassay of SARS-CoV-2 antibody with simultaneously high sensitivity and specificity

Jia, Jianghua; Ao, Lijiao; Luo, Yongxin; Liao, Tao; Huang, Liang; Zhuo, Dinglv; Jiang, Chenxing; Wang, Jing; Hu, Jun

doi:10.1016/j.bios.2021.113810

Cited by 31 publications

(28 citation statements)

References 46 publications

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“…Jia et al assembled 3D QD assembly labels using dendritic silica and OA-capped CdSe/ CdS/ZnS QDs and used it for the detection of SARS-CoV-2. [170] The synthesized 3D QD assembly labels achieved 100% sensitivity and 100% specificity in over 300 negative and 97 positive samples, confirmed by polymerase chain reaction (PCR). Other QD-related SARS-CoV-2 assays also achieved excellent performance.…”

Section: Detection Of Virusmentioning

confidence: 84%

Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Piao

Yang

Cui

et al. 2022

Adv Funct Materials

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With the rapid progress in nanomaterials and biochemistry, there has been an explosion of interest in biomolecule-modified quantum dots (QDs) for biomedical applications. Metal chalcogenide quantum dots (MCQDs), as the most widely studied QDs, have attracted tremendous attention in the biomedical field on account of their unique and excellent optical properties and the ease of biomolecular modifications. Herein, important advances in MCQDs over recent years are reviewed, from materials design to biomedical applications. Especially, this review focuses on the challenges encountered in the applications of MCQDs in biomedical fields and how these problems can be solved by rational design of synthesis methods and modifications, which have opened a universal route to develop the functionalized MCQDs. Moreover, recent processes in bioimaging, biosensing, and cancer therapy based on MCQDs are examined, including the rapid detection and diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This review provides broad insights into MCQDs in the biomedical field and will inspire material researchers to develop MCQDs in the future.

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Section: Detection Of Virusmentioning

confidence: 84%

Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Piao

Yang

Cui

et al. 2022

Adv Funct Materials

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“…S2C and D , under the blocking effect of casein, the magnetic T/C and the fluorescent T/C of negative sample were decreased significantly. This might be because the non-specific interaction between the IMagDQD with the antibody on NC membrane could be effectively blocked by macromolecular protein [ 38 ]. Fig.…”

Section: Resultsmentioning

confidence: 99%

Magnetic/fluorescent dual-modal lateral flow immunoassay based on multifunctional nanobeads for rapid and accurate SARS-CoV-2 nucleocapsid protein detection

Xie

Feng²,

Pei³

et al. 2022

Analytica Chimica Acta

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“…The gold-based lateral flow immunoassay (GLFIA) commonly applied in antibody/serological assays and risks exposing false-negative and false-positive interpretations is not suitable for large-scale population screening. Jia et al [ 23 ] constructed a novel fluorescent lateral flow immunoassay (LFIA), which, to overcome the problem of false negatives, using hydrophobic quantum dots to ensure single-labeled high-intensity fluorescence and to overcome the problem of false positives, a double antigen sandwich construction utilizing labeled/immobilized SARS-CoV-2 RBD. Analysis of nearly 400 clinical samples yielded 100% sensitivity and 100% specificity.…”

Section: Current Trends In Covid-19 Diagnostics: From Centralized Lab...mentioning

confidence: 99%

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

Zhang

Feng

et al. 2022

Microchim Acta

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This review focuses on critical scientific barriers that the field of point-of-care (POC) testing of SARS-CoV-2 is facing and possible solutions to overcome these barriers using functional nucleic acid (FNA)-based technology. Beyond the summary of recent advances in FNA-based sensors for COVID-19 diagnostics, our goal is to outline how FNA might serve to overcome the scientific barriers that currently available diagnostic approaches are suffering. The first introductory section on the operationalization of the COVID-19 pandemic in historical view and its clinical features contextualizes essential SARS-CoV-2-specific biomarkers. The second part highlights three major scientific barriers for POC COVID-19 diagnosis, that is, the lack of a general method for (1) designing receptors of SARS-CoV-2 variants; (2) improving sensitivity to overcome false negatives; and (3) signal readout in resource-limited settings. The subsequent part provides fundamental insights into FNA and technical tricks to successfully achieve effective COVID-19 diagnosis by using in vitro selection of FNA to overcome receptor design barriers, combining FNA with multiple DNA signal amplification strategies to improve sensitivity, and interfacing FNA with portable analyzers to overcome signal readout barriers. This review concludes with an overview of further opportunities and emerging applications for FNA-based sensors against COVID-19. Graphical abstract

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Quantum dots assembly enhanced and dual-antigen sandwich structured lateral flow immunoassay of SARS-CoV-2 antibody with simultaneously high sensitivity and specificity

Cited by 31 publications

References 46 publications

Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Magnetic/fluorescent dual-modal lateral flow immunoassay based on multifunctional nanobeads for rapid and accurate SARS-CoV-2 nucleocapsid protein detection

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

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