Quantum Dots Are Phagocytized by Macrophages and Colocalize With Experimental Gliomas

Jackson, Heather; Muhammad, Osman; Daneshvar, Hamid; Nelms, Jennifer; Popescu, Alexandra; Vogelbaum, Michael A.; Bruchez, Marcel P.; Toms, Steven

doi:10.1227/01.neu.0000255334.95532.dd

Cited by 93 publications

(81 citation statements)

References 29 publications

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“…We have shown that QDs or conjugated QDs accumulated inside the sinusoids in the liver and were subsequently phagocytosed by Kupffer cells. Our data were consistent with others' findings that QDs in the liver tend to be engulfed by macrophages (Jackson et al, 2007). However, when we deliberately depleted macrophages by clodronate liposomes, we still observed that QDs accumulated in the REScontaining organs with similar kinetics.…”

Section: Discussionsupporting

confidence: 93%

“…It has been a consistent observation that systemic administration of pure, non-targeted QDs, and cell-targeted QDs, accumulates in substantial quantities in the liver, spleen, lymph nodes, and bone marrow in animal model systems (Ballou et al, 2004(Ballou et al, , 2007Gao et al, 2004;Cai et al, 2006;Jackson et al, 2007;Diagaradjane et al, 2008;Li and Huang, 2008). These organs all contain the RES system, where macrophage cells reside.…”

Section: Discussionmentioning

confidence: 94%

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Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore

Zhang

Zeng

et al. 2009

Br J Cancer

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BACKGROUND: The type I insulin-like growth factor receptor (IGF1R) is a transmembrane tyrosine kinase involved in cancer proliferation, survival, and metastasis. METHODS: In this study, we used two different fluorescent technologies (small-molecule fluorophores and quantum dot (QD) nanoparticles) to detect receptor expression and its downregulation by antibodies in vivo. RESULTS: After conjugation with AVE-1642, a humanised anti-IGF1R monoclonal antibody, both QDs (705 nm) or Alexa 680 (smallmolecule fluorophore) detected expression and downregulation of IGF1R in vitro. To examine their utility in vivo, either AVE-1642 conjugates were intravenously delivered to mice bearing xenograft tumours of mouse embryo fibroblasts expressing human IGF1R or MCF-7 human breast cancer cells. Quantum dot fluorescence was mainly localised to the reticuloendothelial system in several organs and engulfed by macrophages, with only very small amount of QDs detected in the xenograft tumours. Depletion of macrophages by clodronate liposomes did not alter the nonspecific uptake of QDs. In contrast, AVE-1642-conjugated Alexa 680 solely targeted to xenograft tumour and was able to detect IGF1R downregulation, with little nonspecific targeting to other tissues or organs in mice. CONCLUSION: Taken together, our data suggest that small-molecule fluorophores, not QDs, are suitable to detect the expression and downregulation of IGF1R in vivo.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 94%

Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore

Zhang

Zeng

et al. 2009

Br J Cancer

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“…The electron micrographs also demonstrated that glioblastoma cell lineages internalize the QDs, expressing them The same process of tumor QD marking was described by Jackson et al, 30 wherein the nanocrystals were phagocytosed by macrophages and microglia, thus infiltrating experimental gliomas. Therefore, their study suggests the use of nonconjoined nanocrystals to assist the imaging system for surgical termination or glioblastoma tumor biopsies.…”

Section: Discussionsupporting

confidence: 70%

The Ultrastructural Study of Tumorigenic Cells Using Nanobiomarkers

Pavon

Marti

Sibov

et al. 2010

Cancer Biotherapy and Radiopharmaceuticals

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Despite recent advances, patients with malignant brain tumors still have a poor prognosis. Glioblastoma (WHO grade 4 astrocytoma), the most malignant brain tumor, represents 50% of all astrocytomas, with a median survival rate of <1 year. It is, therefore, extremely important to search for new diagnostic and therapeutic approaches for patients with glioblastoma. This study describes the application of superparamagnetic nanoparticles of iron oxide, as well as monoclonal antibodies, of immunophenotypic significance, conjoined to quantum dots for the ultrastructural assessment of glioblastoma cells. For this proposal, an immunophenotypic study by flow cytometry was carried out, followed by transmission electron microscopy analysis. The process of tumor cell labeling using nanoparticles can successfully contribute to the identification of tumorigenic cells and consequently for better understanding of glioblastoma genesis and recurrence. In addition, this method may help further studies in tumor imaging, diagnosis, and prognostic markers detection.

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“…[19] Contrastingly, Jackson et al show intracellular sequestering. [20] Some decrease of intracellular QD occurred in vitro with KC (Fig. 2B).…”

mentioning

confidence: 86%

Exploring Primary Liver Macrophages for Studying Quantum Dot Interactions with Biological Systems

et al. 2010

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Quantum Dots Are Phagocytized by Macrophages and Colocalize With Experimental Gliomas

Cited by 93 publications

References 29 publications

Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore

Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore

The Ultrastructural Study of Tumorigenic Cells Using Nanobiomarkers

Exploring Primary Liver Macrophages for Studying Quantum Dot Interactions with Biological Systems

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