Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B<sub>1</sub>and amantadine

Zhang, Kezhuo; Cai, Huadong; Lu, Min; Wei, Daixian; Yin, Jiaqi; Ding, Nengshui; Lai, Weihua; Peng, Juan

doi:10.1080/09540105.2022.2080188

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…Immunoassays are widely used for screening and preliminary analysis of mycotoxins due to their advantages of high throughput, economy, and simplicity (Zhao et al., 2019). Based on the specific affinity between an antigen and an antibody (Zhang et al., 2022), major immunoassay methods include enzyme‐linked immunosorbent assay (ELISA) (Drijvers et al., 2017), lateral flow assay (LFAs) (Charlermroj et al., 2021), fluorescence polarization assay (Huang et al., 2020), and radioimmunoassay (Ayoub et al., 2016). Notably, LFAs for detecting mycotoxins have matured to commercialization due to their simplicity, portability, sensitivity, selectivity, and rapidity (Pan et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

Li,

Jallow,

Nidiaye

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxins are dangerous human and animal health‐threatening secondary fungal metabolites that can be found in various food and agricultural products. Several countries have established regulations to restrict their presence in food and agricultural products destined for human and animal consumption. Consequently, the need to develop highly sensitive and smart detection systems was recognized worldwide. Lateral flow assay possesses the advantages of easy operation, rapidity, stability, accuracy, and specificity, and it plays an important role in the detection of mycotoxins. Nevertheless, strategies to comprehensively improve the sensitivity of lateral flow assay to mycotoxins in food have rarely been highlighted and discussed. In this article, a comprehensive overview was presented on the application of lateral flow assay in mycotoxin detection in food samples by highlighting the principle of lateral flow assay, presenting a detailed discussion on various analytical performance‐improvement strategies, such as the development of high‐affinity recognition reagents, immunogen immobilization methods, and signal amplification. Additionally, a detailed discussion on the various signal analyzers and interpretation approaches was provided. Finally, current hurdles and future perspectives on the application of lateral flow assay in the detection of mycotoxins were discussed.

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Section: Introductionmentioning

confidence: 99%

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

Li,

Jallow,

Nidiaye

et al. 2023

Comp Rev Food Sci Food Safe

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“…The preparation of anti-mercury monoclonal antibody (mAb) for mercury detection has been reported [ 11 , 12 ]. However, studies reported low sensitivity and cross-reactivity with copper [ 13 ]. Additionally, there are no reports on using immunoassay test strips in grains [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

Song

et al. 2022

Biosensors

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Mercury is a major pollutant in food crops. In this study, we synthesized an anti-mercury monoclonal antibody (mAb; IC50 was 0.606 ng mL−1) with high sensitivity and specificity and different immunogens and coating antigens and developed an immuno-chromatographic assay (ICA) for the detection of mercury in rice. The ICA strip had a visible detection limit of 20 ng g−1 and a cut-off value of 500 ng g−1 in rice. The performance of the ICA strip was consistent with that of ICP-MS and ic-ELISA. The recoveries of mercury in rice ranged from 94.5% to 113.7% with ic-ELISA and from 93.6% to 116.45% with ICP-MS. Qualitative analysis by ICA can be obtained with the naked eye. The ICA strip is an effective and practical method for the rapid and high-throughput determination of mercury in rice.

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Recent advancements in bioreceptors and materials for biosensors

Kumar,

Mahato

2024

Biosensors in Precision Medicine

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Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B₁and amantadine

Cited by 7 publications

References 30 publications

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

Recent advancements in bioreceptors and materials for biosensors

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Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1and amantadine

Cited by 7 publications

References 30 publications

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

Recent advancements in bioreceptors and materials for biosensors

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Product

Resources

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Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B₁and amantadine