Quantum dot-enhanced detection of dual short RNA sequences via one-step template-dependent surface hybridization

Song, Wenqing; Lau, Choiwan; Lu, Jianzhong

doi:10.1016/j.aca.2012.05.031

Cited by 11 publications

(14 citation statements)

References 34 publications

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“…35 For the calculation of T m values we did not take into account changes due to attachment of the capture DNA to oligos, which may lead to an increased T m . 26 However, even if the T m values were slightly larger this was not problematic for 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 our assays because our empiric optimization led to enhanced FRET sensitization in the presence of miRNA targets. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 QD3) 36 and the detection filter transmission bandwidth and Tb PL spectral crosstalk to the QD detection chan...…”

Section: -Mirna Double Strands Of 20a and 20b Have Only 2 Base Pair Mmentioning

confidence: 97%

Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer

2015

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The detection of next generation microRNA (miRNA) biomarkers has become a highly important aspect for clinical diagnostics. We use multiplexed Förster resonance energy transfer (FRET) between a luminescent Tb complex and three different semiconductor quantum dots (QDs) to sensitively detect three different miRNAs from a single 150 μL sample with ca. 1 nM (subpicomol) detection limits. The rapid and amplification-free mix-and-measure assay format is based on careful design of miRNA base pairing and stacking to selectively detect different miRNAs with very strong sequence homologies. Clinical applicability is demonstrated by sensitive multiplexed quantification of three miRNAs at low (2 to 10 nM) and varying concentrations in samples that contained up to 10% serum.

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Section: -Mirna Double Strands Of 20a and 20b Have Only 2 Base Pair Mmentioning

confidence: 97%

Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer

2015

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“…Song and his co-workers established a ligase-free sensor with one-step hybridization based on quantum dots functionalized reporter DNA for the specific detection of miRNA mimics (the dual short RNA), and demonstrated that quantum dots-modified reporter probes could increase the melting temperature [42]. In the present paper, two sizes of QDs with emission wavelengths at 605 nm and 655 nm functionalized respectively by SA and anti-FAM (denoted as SA-QDs and anti-FAM QDs) were bound to biotinylated oligonucleotides reporter A and FAMreporter B, in which one end was complementary to the capture probe immobilized on 96-well plate, and the other end was complementary to the short RNA target (Fig.…”

Section: Qdsmentioning

confidence: 99%

“…Renilla luciferase) to inorganic nanostructures( eg. QDs and AgNCs ) and other nonfluorescent nanomaterials conjugated or encapsuled with the fluorescent dyes [41][42][43][44].…”

Section: Fluorescence-based Microrna Detectionmentioning

confidence: 99%

Nanoparticle-based Optical Detection of MicroRNA

Zhang¹,

Cui²

2013

Nano BioMed ENG

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MiRNAs are valuable biomarkers for diagnosis and prognosis of cancers, so it is imperative to develop rapid, sensitive, high throughput miRNA detection methods. In this review article, the current nanoparticle-based miRNA detection by optical technology was summarized from the following aspects about fluorescence, scattering and colorimetry. And the emphasis is laid on employing quantum dots (QDs) and silver nanoclusters (AgNCs)based fluorescence and silver nanorod-based surface enhanced Raman scattering (SERS) to detect miRNAs. Nanoparticle-based optical detection is prospective for multiplexed miRNA detection or in vivo imaging.

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“…In this regard, our group in 2010 reported a new strategy of template-dependent hybridization for short-length DNA detection based on target-enabled hybridization of a capture probe with a long reporter probe [22] . In 2012, we further employed a template-dependent surface hybridization using quantum dot-enhanced methodology to detect dual short RNA sequences [23] . A multiplexed “mix-and-measure” method would be highly desirable for measuring the expression levels of miRNAs.…”

Section: Introductionmentioning

confidence: 99%

Duplex microRNAs assay based on target-triggered universal reporter hybridization

Wang

Sun

Lau

et al. 2018

Journal of Pharmaceutical Analysis

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In this paper, we designed and evaluated a duplex detection strategy for microRNAs (miRNAs) using universal probe-based target-triggered double hybridization and fluorescent microsphere-based assay system (xMAP array). In the absence of target miRNA, reporter DNA cannot hybridize stably with the immobilized capture DNA due to its low melting temperature. Only after adding target miRNA, can reporter probe hybridize with capture probe to form a stable three-component complex. This target-triggered stable hybridization makes this method possible for highly selective and sensitive detection of multiple miRNAs. We exemplified a quantitative detection of duplex miRNAs with a limit of detection of 40 pM. The xMAP array platform holds the potential of extending this approach to simultaneous detection of up to 100 miRNA targets. Considering the simplicity, rapidity and multiplexing, this work promised a potential detection of multiple miRNA biomarkers for early disease diagnosis and prognosis.

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Quantum dot-enhanced detection of dual short RNA sequences via one-step template-dependent surface hybridization

Cited by 11 publications

References 34 publications

Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer

Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer

Nanoparticle-based Optical Detection of MicroRNA

Duplex microRNAs assay based on target-triggered universal reporter hybridization

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