Quantum dot clusters as self-assembled antennae with phycocyanine and phycobilisomes as energy acceptors

Grzyb, Joanna; Walczewska-Szewc, Katarzyna; Sławski, Jakub; Trojnar, Martyna

doi:10.1039/d1cp03347f

Cited by 4 publications

(10 citation statements)

References 38 publications

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“…This technique was successfully used for proving protein−protein 51 as well as protein−nanoparticle interaction. 33 The covalent binding fixes the components of the nanohybrid assembly in place. However, the distribution of components is stochastic, which may result in spatial gaps preventing resonance energy transfer.…”

Section: ■ Discussionmentioning

confidence: 99%

“…QDs were already shown to be able to donate energy to PBSr located in a short enough proximity. , The energy transfer in our system was proven by FLIM measurement, showing the fingerprint of FRET, the increase in acceptor τ. This technique was successfully used for proving protein–protein as well as protein–nanoparticle interaction …”

Section: Discussionmentioning

confidence: 99%

“…For both photosynthetic antennae, QD with optimal emission properties was used (QD530 or QD570 for PBSr and LHCII, respectively), prompting efficient energy transfer if only a close distance between the donor and the acceptor was assured. QDs were already shown to be able to donate energy to PBSr located in a short enough proximity. , The energy transfer in our system was proven by FLIM measurement, showing the fingerprint of FRET, the increase in acceptor τ. This technique was successfully used for proving protein–protein as well as protein–nanoparticle interaction …”

Section: Discussionmentioning

confidence: 99%

“…For example, it is expected that outside of the space of close mutual proximity, there is a very low chance for energy transfer between QDs and PBSr/LHCII. For energy transfer to occur, QDs and PBS need to be connected covalently or assembled into electrostatically associated clusters . The assembly studied here of QDs and PBSr/LHCII on a nanotube is a form of covalent connection that offers more specific options for the complex architecture.…”

Section: Introductionmentioning

confidence: 99%

“…For energy transfer to occur, QDs and PBS need to be connected covalently 32 or assembled into electrostatically associated clusters. 33 The assembly studied here of QDs and PBSr/LHCII on a nanotube is a form of covalent connection that offers more specific options for the complex architecture. To some extent, the stoichiometry as well as organization of nanohybrid components may be controlled.…”

Section: Introductionmentioning

confidence: 99%

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Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Sławski,

Maciejewski,

Szukiewicz

et al. 2023

ACS Omega

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The construction of artificial systems for solar energy harvesting is still a challenge. There needs to be a light-harvesting antenna with a broad absorption spectrum and then the possibility to transfer harvested energy to the reaction center, converting photons into a storable form of energy. Bioinspired and bioderivative elements may help in achieving this aim. Here, we present an option for light harvesting: a nanobiohybrid of colloidal, semiconductor quantum dots (QDs) and natural photosynthetic antennae assembled on the surface of a carbon nanotube. For that, we used QDs of cadmium telluride and cyanobacterial phycobilisome rods (PBSr) or light-harvesting complex II (LHCII) of higher plants. For this nanobiohybrid, we confirmed composition and organization using infrared spectroscopy, X-ray photoelectron spectroscopy, and high-resolution confocal microscopy. Then, we proved that within such an assembly, there is a resonance energy transfer from QD to PBSr or LHCII. When such a nanobiohybrid was further combined with thylakoids, the energy was transferred to photosynthetic reaction centers and efficiently powered the photosystem I reaction center. The presented construct is proof of a general concept, combining interacting elements on a platform of a nanotube, allowing further variation within assembled elements.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Sławski,

Maciejewski,

Szukiewicz

et al. 2023

ACS Omega

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Bio-catalytic nanoparticle shaping for preparing mesoscopic assemblies of semiconductor quantum dots and organic molecules

Akter,

Kirkwood,

Zaman

et al. 2024

Nanoscale Horiz.

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Protein/Protein and Quantum Dot/Protein Organization in Sequential Monolayer Materials Studied Using Resonance Energy Transfer

Sławski,

Walczewska-Szewc,

Grzyb

2023

Applied Sciences

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Controlled junctions of proteins and nanomaterials offer multiple potential applications in the further construction of nanobiodevices. One of the possible junction types is a set of sequential monolayers of various components deposited on a given substrate. The advantage of such an organization is its high sensitivity, resulting from a huge surface covered by molecules or particles. What is more, the molecules/particles adsorbed on a substrate might be easier to handle than the assay in a cuvette. For further application, there should be crosstalk between monolayers; this is defined by the type of individuals forming a complex system. Here, we are studying, using mainly confocal microscopy and FLIM imaging, crosstalk through resonance energy transfer. The sequential monolayers of fluorescent proteins and CdTe quantum dots were deposited on a convenient substrate, a polyvinylidene difluoride membrane. First, we found that the degree of coverage is lower in the second monolayer. Hence, by manipulating the order of deposition, we obtained a system with a varied yield of resonance energy transfer with a donor excess or an acceptor excess. For a deeper understanding of the energy transfer and its limitations in this system influencing the assay pursuit, we utilized Monte Carlo computation. We found that, indeed, the distance between the monolayers, as well as the degree of coverage, is crucial. With the results of the simulation, we might estimate the relative degree of coverage in our sequential monolayers. We also found that in quantum-dots/protein-composed systems, the yield is stronger than predicted by Monte Carlo simulation. Hence, there should be protein reorientation on the nanoparticle surface, leading to such an effect. Finally, we showed that the yield of resonance energy transfer may be modulated by the external application of poly-L-lysines. These chemicals influenced QD fluorescence but not protein fluorescence and might be used, therefore, as a trigger or a switch in nanobiodevices employing those types of sequential monolayers.

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Quantum dot clusters as self-assembled antennae with phycocyanine and phycobilisomes as energy acceptors

Abstract: In this study, we investigated an experimental and Monte-Carlo computational characterization of self-assembled antennae built of CdTe colloidal quantum dots (QDs). These clusters provide efficient excitation of phycocyanine (PC) or...

Cited by 4 publications

References 38 publications

Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Bio-catalytic nanoparticle shaping for preparing mesoscopic assemblies of semiconductor quantum dots and organic molecules

Protein/Protein and Quantum Dot/Protein Organization in Sequential Monolayer Materials Studied Using Resonance Energy Transfer

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