Quantum Dot Based Biotracking and Biodetection

Zhang, Lijuan; Li, Xia; Xie, Hai‐Yan; Zhang, Zhiling; Pang, Dai‐Wen

doi:10.1021/acs.analchem.8b04721

Cited by 64 publications

(42 citation statements)

References 213 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The excellent optical properties make QDs unparalleled in single-molecule/virus tracking. Single molecules/viruses illuminated with QDs can be rapidly and continuously tracked for a long time (7), and their interactions with multiple other molecules can be monitored simultaneously (8,9), providing more detailed information to dissect cellular events than with those labeled by other fluorophores. Thanks to these advantages, QDs have been widely used to label proteins for single-molecule-tracking studies (10)(11)(12)(13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking

Zhang

Wang

et al. 2020

mBio

Self Cite

View full text Add to dashboard Cite

Quantum dots (QDs) possess optical properties of superbright fluorescence, excellent photostability, narrow emission spectra, and optional colors. Labeled with QDs, single molecules/viruses can be rapidly and continuously imaged for a long time, providing more detailed information than when labeled with other fluorophores. While they are widely used to label proteins in single-molecule-tracking studies, QDs have rarely been used to study virus infection, mainly due to a lack of accepted labeling strategies. Here, we report a general method to mildly and readily label enveloped viruses with QDs. Lipid-biotin conjugates were used to recognize and mark viral lipid membranes, and streptavidin-QD conjugates were used to light them up. Such a method allowed enveloped viruses to be labeled in 2 h with specificity and efficiency up to 99% and 98%, respectively. The intact morphology and the native infectivity of viruses were preserved. With the aid of this QD labeling method, we lit wild-type and mutant Japanese encephalitis viruses up, tracked their infection in living Vero cells, and found that H144A and Q258A substitutions in the envelope protein did not affect the virus intracellular trafficking. The lipid-specific QD labeling method described in this study provides a handy and practical tool to readily “see” the viruses and follow their infection, facilitating the widespread use of single-virus tracking and the uncovering of complex infection mechanisms. IMPORTANCE Virus infection in host cells is a complex process comprising a large number of dynamic molecular events. Single-virus tracking is a versatile technique to study these events. To perform this technique, viruses must be fluorescently labeled to be visible to fluorescence microscopes. The quantum dot is a kind of fluorescent tag that has many unique optical properties. It has been widely used to label proteins in single-molecule-tracking studies but rarely used to study virus infection, mainly due to the lack of an accepted labeling method. In this study, we developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates and recognizing these lipid-biotin conjugates with streptavidin-quantum dot conjugates. It is not only applicable to normal viruses, but also competent to label the key protein-mutated viruses and the inactivated highly virulent viruses, providing a powerful tool for single-virus tracking.

show abstract

mentioning

confidence: 99%

Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking

Zhang

Wang

et al. 2020

mBio

Self Cite

View full text Add to dashboard Cite

show abstract

“…The QDs with NIR-I excitation and NIR-II emission can effectively solve this problems. [193] Recently, NIR-II QDs with a QY high enough for sensitive biodetection and high-resolution bioimaging have been developed. For example, Dai et al synthesized PbS@CdS QDs with a bright emission at about 1600 nm.…”

Section: High Signal Valuementioning

confidence: 99%

Functional Micro‐/Nanomaterials for Multiplexed Biodetection

Liu

Cui

et al. 2021

Advanced Materials

View full text Add to dashboard Cite

When analyzing biological phenomena and processes, multiplexed biodetection has many advantages over single-factor biodetection and is highly relevant to both human health issues and advancements in the life sciences. However, many key problems with current multiplexed biodetection strategies remain unresolved. Herein, the main issues are analyzed and summarized: 1) generating sufficient signal to label targets, 2) improving the signal-to-noise ratio to ensure total detection sensitivity, and 3) simplifying the detection process to reduce the time and labor costs of multiple target detection. Then, available solutions made possible by designing and controlling the properties of micro-and nanomaterials are introduced. The aim is to emphasize the role that micro-/nanomaterials can play in the improvement of multiplexed biodetection strategies. Through analyzing existing problems, introducing state-of-the-art developments regarding relevant materials, and discussing future directions of the field, it is hopeful to help promote necessary developments in multiplexed biodetection and associated scientific research.

show abstract

“…The excellent optical properties make QDs unparalleled in single-molecule/virus tracking. Single molecules/viruses illuminated with QDs can be rapidly and continuously tracked for a long time [5, 6], and their interactions with multiple other molecules can be monitored simultaneously [7-9], providing more detailed information to dissect cellular events than those labeled by other fluorophores. Thanks to these advantages, QDs have been widely used to label proteins for single-molecule tracking studies [10-17].…”

Section: Introductionmentioning

confidence: 99%

Lipid-specific labeling of enveloped viruses with quantum dots for single-virus tracking

Zhang

Wang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

24Quantum dots (QDs) possess optical properties of superbright fluorescence, excellent photostability, narrow 25 emission spectra, and optional colors. Labeled with QDs, single molecules/viruses can be rapidly and 26 continuously imaged for a long time, providing more detailed information than labeled with other 27 fluorophores. While they are widely used to label proteins in single-molecule tracking studies, QDs have 28 rarely been used to study virus infection, mainly due to lack of accepted labeling strategies. Here, we report 29 a general method to mildly and readily label enveloped viruses with QDs. Lipid-biotin conjugates were used 30 to recognize and mark viral lipid membranes, and streptavidin (SA)-QD conjugates were used to light them 31 up. Such a method allowed enveloped viruses to be labeled in 2 hours with specificity and efficiency up to 32 99% and 98%. The intact morphology and the native infectivity of viruses could be furthest preserved. With 33 the aid of this QD labeling method, we lit wild-type (WT) and mutant Japanese encephalitis virus (JEV) up, 34 tracked their infection in living Vero cells, and found that H144A and Q258A substitutions in the envelope 35 (E) protein didn't affect the virus intracellular trafficking. The lipid-specific QD labeling method described 36 in this study provides a handy and practical tool to readily "see" the viruses and follow their infection, 37 facilitating the widespread use of single-virus tracking and the uncovering of complex infection 38 mechanisms. 39Author summary 40 Virus infection in host cells is a complex process comprising a large number of dynamic molecular events. 41Single-virus tracking is a versatile technique to study these events. To perform this technique, viruses must 42 be fluorescently labeled to be visible to fluorescence microscopes. Quantum dot is a kind of fluorescent tags 43 that has many unique optical properties. It has been widely used to label proteins in single-molecule tracking 44 studies, but rarely used to study virus infection, mainly due to lack of accepted labeling method. In this 45 study, we developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped 46 viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates and recognizing 47 these lipid-biotin conjugates with streptavidin-quantum dot conjugates. Such a method is superior to the 48 commonly used DiD/DiO labeling and the other QD labeling methods. It is not only applicable to normal 3 49 viruses, but also competent to label the key protein-mutated viruses and the inactivated high virulent viruses, 50 providing a powerful tool for single-virus tracking. 51 Introduction 52 Single-particle tracking is a powerful tool to study the dynamic molecular events in living cells. An essential 53 prerequisite to perform this technique is fluorescently labeling the targets. In the past decade, various 54 fluorescent tags such as organic dyes [1], fluorescent proteins [2], metal complex of dppz [3], and QDs ...

show abstract

Quantum Dot Based Biotracking and Biodetection

Cited by 64 publications

References 213 publications

Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking

Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking

Functional Micro‐/Nanomaterials for Multiplexed Biodetection

Lipid-specific labeling of enveloped viruses with quantum dots for single-virus tracking

Contact Info

Product

Resources

About