quanTLC, an online open-source solution for videodensitometric quantification

Fichou, Dimitri; Morlock, Gertrud E.

doi:10.1016/j.chroma.2018.05.027

Cited by 26 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an example, the AChE inhibition autogram was evaluated using the open-source quanTLC software [ 28 ]. The variation of the activity of the AChE inhibiting compound zone 21 across all 12 different Coffea arabica samples ( Figure 3 , framed in yellow) was 6.3% ( %RSD , n = 12, green channel signal).…”

Section: Resultsmentioning

confidence: 99%

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

et al. 2022

Self Cite

View full text Add to dashboard Cite

Ethiopian honey is used not only as food but also for treatment in traditional medicine. For its valorization, bioactive compounds were analyzed in nine types of monofloral Ethiopian honey. Therefore, a non-target effect-directed profiling was developed via high-performance thin-layer chromatography combined with multi-imaging and planar effect-directed assays. Characteristic bioactivity profiles of the different honeys were determined in terms of antibacterial, free-radical scavenging, and various enzyme inhibitory activities. Honeys from Hypoestes spp. and Leucas abyssinica showed low activity in all assays. In contrast, others from Acacia spp., Becium grandiflorum, Croton macrostachyus, Eucalyptus globulus, Schefflera abyssinica, Vernonia amygdalina, and Coffea arabica showed more intense activity profiles, but these differed depending on the assay. In particular, the radical scavenging activity of Croton macrostachyus and Coffea arabica honeys, the acetylcholinesterase-inhibiting activity of Eucalyptus globulus and Coffea arabica honeys, and the antibacterial activity of Schefflera abyssinica honey are highlighted. Bioactive compounds of interest were further characterized by high-resolution mass spectrometry. Identifying differences in bioactivity between mono-floral honey types affects quality designation and branding. Effect-directed profiling provides new insights that are valuable for food science and nutrition as well as for the market, and contributes to honey differentiation, categorization, and authentication.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…It may provide the platform for easy mass spectra readout and processing in a facilitated workflow to automatically define target zones and to combine all possible datasets from HPTLC plates for an intelligent evaluation. For example, quanTLC for peak detection and videodensitometric quantification or superior image evaluation by artificial neural networks or powerful cluster analysis of the recorded HPTLC‐MS spectra were recently developed modern tools that are helpful for analysts in the field of TLC/HPTLC.…”

Section: Discussionmentioning

confidence: 99%

Open‐source add‐on kit for automation of zone elution in planar chromatography

Häbe

Morlock

2020

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

Rationale High‐throughput capacity, maximal efficiency and automation are missing key features for thin‐layer chromatography/high‐performance thin‐layer chromatography‐mass spectrometry (TLC/HPTLC‐MS) hyphenations. So far, commercial interfaces have been operated without an automated positioning system. Hence, a 3D‐printed, fully automated, open‐source add‐on user interface was built for elution‐head‐based TLC/HPTLC‐MS. Methods Precise plate movement, batch‐wise zone elution, and cleaning processes were automated and synchronized with MS data acquisition. The elution head cleaning was made adjustable in intensity and interval, and remaining elution solvent or particles were thoroughly blown out of the MS transfer line. An adjustable short gas beam across the elution zone was integrated to avoid contamination or leakage by released layer particles, or zone distortion by residual eluent flow when lifting the elution head. Results By clicking on zones on the chromatogram, these were selected for consecutive automated positioning below the elution head, sealing and elution. Mean spatial deviations of the positioning on 294 target zones were determined to be 160 μm for track‐wise and 190 μm for randomized zone positioning order. Reproducibility of the elution of butyl paraben zones (5.4%, 10 ng/band, n = 70) and its quantitative performance (R2 = 0.992–0.999, 5–50 ng/band) were proven. Conclusions The stand‐alone electronic system design and the compact footprint were achieved by a minimalistic positioning system and internally installed valves. The open‐source OC_manager software opened new perspectives in terms of combining MS data evaluation with previous HPTLC results. The successfully demonstrated TLC/HPTLC‐MS automation presents a highly efficient, user‐independent hyphenation for a broad range of application fields.

show abstract

“…Concerning data analysis, uncorrected images are available in the simple OC_manager software environment. Enhanced data processing as well as an easy links to other data analysis software like quanTLC and rTLC will be valuable.…”

Section: Discussionmentioning

confidence: 99%

Office Chromatography: Miniaturized All-in-One Open-Source System for Planar Chromatography

Fichou

Morlock

2018

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

Current high-performance-thin-layer-chromatography instrumentation is offline and stepwise automated. However, moderate miniaturization offers many advantages and together with the transfer of modern print and media technologies to the field of chromatography (office chromatography) it opens up new avenues. This is demonstrated in an allin-one open-source system developed for planar chromatography and especially for ultrathin-layer chromatography. Using an InkShield board to control a thermal inkjet cartridge, picoliter drops were printed at a resolution of 96 dpi on the adsorbent layer. Using Marlin, a popular firmware in 3D printing, Cartesian movement of the print head was made possible for full control of the printing process. Open-source software was developed to control the device in each operation step. Sample solutions and mobile phase were inkjet-printed, exemplarily shown for the analysis of dye-or paraben-mixture solutions. Light-emitting diodes (LEDs) were investigated for documentation. For example, deep UV LEDs gave access to 254 nm light, and RGB LEDs gave access to the visible-light range. Calibration functions with correlation coefficients superior to 0.999 were obtained by videodensitometry. The developed modular open-source hardware was compact (26 × 31 × 26 cm 3 ), light (<3 kg), and affordable (€810). For the given analyses, the footprint of current instrumentation needed was miniaturized by a factor of 9. The highly reduced material design complies with green chemistry and lean laboratory. The design and instruction to reproduce the all-in-one open-source system were made freely available at https://github.com/OfficeChromatography. It is intended to boost progress and understanding by the nature of do it yourself.

show abstract

quanTLC, an online open-source solution for videodensitometric quantification

Cited by 26 publications

References 17 publications

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

Open‐source add‐on kit for automation of zone elution in planar chromatography

Office Chromatography: Miniaturized All-in-One Open-Source System for Planar Chromatography

Contact Info

Product

Resources

About