Quantized Folding of Plasmid DNA Condensed with Block Catiomer into Characteristic Rod Structures Promoting Transgene Efficacy

Osada, Kensuke; Oshima, Hiroki; Kobayashi, Daigo; Doi, Motoyoshi; Enoki, Manabu; Yamasaki, Yuichi; Kataoka, Kazunori

doi:10.1021/ja102739b

Cited by 84 publications

(101 citation statements)

References 40 publications

(53 reference statements)

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“…It was shown that tuning steric forces was critical in obtaining 1-D nanostructures of precisely controlled length, by preventing buckling or condensation of the DNA template. The observations were consistent with the folding model for DNA condensation suggested by Kataoka, 220 derived from a mechanism based on Euler's buckling theory. 221 The templated self-assembly and supramolecular polymerization into 1-D filaments using oligonucleotides is a powerful strategy to produce uniform materials of well-defined size and shape, with a high degree of fidelity.…”

Section: Biomimetic Templated Polymerizationsupporting

confidence: 88%

Controlling supramolecular polymerization through multicomponent self‐assembly

Besenius

2016

J. Polym. Sci. Part A: Polym. Chem.

127

102

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The self-assembly into supramolecular polymers is a process driven by reversible non-covalent interactions between monomers, and gives access to materials applications incorporating mechanical, biological, optical or electronic functionalities. Compared to the achievements in precision polymer synthesis via living and controlled covalent polymerization processes, supramolecular chemists have only just learned how to developed strategies that allow similar control over polymer length, (co)monomer sequence and morphology (random, alternating or blocked ordering). This highlight article discusses the unique opportunities that arise when coassembling multicomponent supramolecular polymers, and focusses on four strategies in order to control the polymer architecture, size, stability and its stimuli-responsive properties: (1) endcapping of supramolecular polymers, (2) biomimetic templated polymerization, (3) controlled selectivity and reactivity in supramolecular copolymerization, and (4) living supramolecular polymerization. In contrast to the traditional focus on equilibrium systems, our emphasis is also on the manipulation of selfassembly kinetics of synthetic supramolecular systems. V C 2016

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Section: Biomimetic Templated Polymerizationsupporting

confidence: 88%

Controlling supramolecular polymerization through multicomponent self‐assembly

Besenius

2016

J. Polym. Sci. Part A: Polym. Chem.

127

102

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“…Increasing the N/P ratio led to ad ecreased electrophoretic mobility of the product bands up to N/P = 1, from whereon the DOPMs migrated towards the cathode (Figure 2a). As imilar discrete folding in PMs has also been observed in double-stranded DNA [49] and suggests that for applications where high rigidity and strict shape control are needed, reinforced multilayer structures should be used. [48] Thet SEM images revealed intact structures where the shape of the DOPMs was strictly dictated by the underlying DNAorigami core.The only exception was the flat RO,which was protected as well, but which was also folded in half along the helical axis in more than 50 %o ft he cases ( Figure S4).…”

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confidence: 77%

Block Copolymer Micellization as a Protection Strategy for DNA Origami

et al. 2017

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DNA nanotechnology enables the synthesis of nanometer-sized objects that can be site-specifically functionalized with a large variety of materials. For these reasons, DNA-based devices such as DNA origami are being considered for applications in molecular biology and nanomedicine. However, many DNA structures need a higher ionic strength than that of common cell culture buffers or bodily fluids to maintain their integrity and can be degraded quickly by nucleases. To overcome these deficiencies, we coated several different DNA origami structures with a cationic poly(ethylene glycol)-polylysine block copolymer, which electrostatically covered the DNA nanostructures to form DNA origami polyplex micelles (DOPMs). This straightforward, cost-effective, and robust route to protect DNA-based structures could therefore enable applications in biology and nanomedicine where unprotected DNA origami would be degraded.

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“…The N/P ratio was â« in our experiments because transfection rates were low at N/P below 10. Interestingly, at N/P ratios of 1.0, an impact on circular plasmid tertiary structure has been reported using a different polycationic polymer (19). Particle size and shape are important variables in the cell biology of endocytosis and phagocytosis (20).…”

Section: Discussionmentioning

confidence: 99%

Plasmid linearization changes shape and efficiency of transfection complexes

Lehner¹,

Wang²,

Hunziker³

2013

European Journal of Nanomedicine

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Abstract:The ability to efficiently transfect plasmid DNA (pDNA) into eukaryotic cells has exerted major impact on scientific research in recent years, and translation to clinical application is ongoing, but challenging. In addi tion to the choice of the delivery vector, the topology of the DNA seems to be a key factor for efficient transfection. The nanostructured DNA/Vector complexes may differ in size, charge, and shape, for example. This study therefore investigated the transfection efficiency of circular versus linearized plasmid DNA using a GFP expressing vector with Lipofectamine2000 and linear 25 kDA polyethylen imine (PEI). Transfection efficiency and cytotoxicity were measured by flow cytometry and fluorescence micro scopy. Shape was determined by transmission electron microscopy. Transfection agent concentrations were cho sen below the toxicity level. We determined the optimal N/P ratio over 48 h by using two different concentrations of plasmid DNA. With the increase of DNA concentra tion and increasing N/P ratio, transfection efficiency also increased. Our results showed a better transfection effi ciency with the circular compared to the linearized DNA, under the same experimental conditions for both Lipo fectamine and PEI. In electron microscopy, there was a notable difference in the shape of the complexes: circu lar DNA had random coil appearance in well compacted, roughly spherical shape, while linearized DNA appeared as wormlike strands, both, when complexed with Lipo fectamine or with polyethyleneimine. This generates the hypothesis that the shape of the transfection particle may be an important factor for successful gene transfer.

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Quantized Folding of Plasmid DNA Condensed with Block Catiomer into Characteristic Rod Structures Promoting Transgene Efficacy

Cited by 84 publications

References 40 publications

Controlling supramolecular polymerization through multicomponent self‐assembly

Controlling supramolecular polymerization through multicomponent self‐assembly

Block Copolymer Micellization as a Protection Strategy for DNA Origami

Plasmid linearization changes shape and efficiency of transfection complexes

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