Electro-optical detection systems face numerous challenges due to the complexity and difficulty of targeting controls for “low, slow and tiny” moving targets. In this paper, we present an optimal model of an advanced n-step adaptive Kalman filter and gyroscope short-term integration weighting fusion (nKF-Gyro) method with targeting control. A method is put forward to improve the model by adding a spherical coordinate system to design an adaptive Kalman filter to estimate target movements. The targeting error formation is analyzed in detail to reveal the relationship between tracking controller feedback and line-of-sight position correction. Based on the establishment of a targeting control coordinate system for tracking moving targets, a dual closed-loop composite optimization control model is proposed. The outer loop is used for estimating the motion parameters and predicting the future encounter point, while the inner loop is used for compensating the targeting error of various elements in the firing trajectory. Finally, the modeling method is substituted into the disturbance simulation verification, which can monitor and compensate for the targeting error of moving targets in real time. The results show that in the optimal model incorporating the nKF-Gyro method with targeting control, the error suppression was increased by up to 36.8% compared to that of traditional KF method and was 25% better than that of the traditional nKF method.